Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room
<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> ...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2016-08-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | http://jmb.tums.ac.ir/index.php/jmb/article/view/179 |
| _version_ | 1818172518109806592 |
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| author | Ramezan Ali Ataee Hassan Araqizade Rezvan Yoosefi Ali Mehrabi Tavana Mohammad Hosein Ataee |
| author_facet | Ramezan Ali Ataee Hassan Araqizade Rezvan Yoosefi Ali Mehrabi Tavana Mohammad Hosein Ataee |
| author_sort | Ramezan Ali Ataee |
| collection | DOAJ |
| description | <div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> This study aimed to evaluate antibacterial effect of <em>Allium sativum,</em> garlic extract on erythromycin and methicillin resistant bacteria isolated from an operating room in a teaching hospital in Tehran, I.R. Iran.</p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> The antibacterial effect of garlic extract was investigated on 70 bacterial strains. The selected isolates were resistant to erythromycin and or methicillin, which were isolated from an operating room. Antibiotic sensitivity was done using an agar well diffusion procedure and either a micro dilution method. Each of the bacterial strains were exposed to concentrations of 4, 8, 12, 16, 20 and 24 µg/ml of garlic extract, separately. The growth rate of the strains was determined by measurement of the inhibition zone diameter, colony count and either by measurement of the optical density.</p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> The results showed that 70 (100%) of the strains in agar well diffusion method were sensitive to 4- 12 µg/ml of garlic extract with MIC 8 µg/ml. While, the results of micro dilution method showed that 40 out of 70 strains were MIC ≥ 12 µg/ml for GE.</p><p><strong><em>Conclusion</em></strong><strong>:<em> </em></strong> The results of this study indicated that the MIC and minimum bactericidal concentration of garlic extract were 8 µg/ml and 16 µg/ml, respectively. These finding indicated that garlic extract inhibit the growth of erythromycin and methicillin resistant bacterial strains.</p><p> </p></td></tr></tbody></table></div> |
| first_indexed | 2024-12-11T19:13:53Z |
| format | Article |
| id | doaj.art-8e1bd71d6de54d9498d3d3bc63b28559 |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-11T19:13:53Z |
| publishDate | 2016-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-8e1bd71d6de54d9498d3d3bc63b285592022-12-22T00:53:43ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812016-08-0151-2714182Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating RoomRamezan Ali Ataee0Hassan Araqizade1Rezvan Yoosefi2Ali Mehrabi Tavana3Mohammad Hosein Ataee4Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.Departments of Anesthesiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.Faculty of Nursing, Baqiyatallah University of Medical Sciences, Tehran, Iran.Health Management Research Center and Department of Medical Microbiology, Baqiyatallah University of Medical Sciences, Tehran, Iran.Applied Microbial Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><strong><em>Background</em></strong><strong>:<em> </em></strong> This study aimed to evaluate antibacterial effect of <em>Allium sativum,</em> garlic extract on erythromycin and methicillin resistant bacteria isolated from an operating room in a teaching hospital in Tehran, I.R. Iran.</p><p><strong><em>Methods</em></strong><strong>:<em> </em></strong> The antibacterial effect of garlic extract was investigated on 70 bacterial strains. The selected isolates were resistant to erythromycin and or methicillin, which were isolated from an operating room. Antibiotic sensitivity was done using an agar well diffusion procedure and either a micro dilution method. Each of the bacterial strains were exposed to concentrations of 4, 8, 12, 16, 20 and 24 µg/ml of garlic extract, separately. The growth rate of the strains was determined by measurement of the inhibition zone diameter, colony count and either by measurement of the optical density.</p><p><strong><em>Results</em></strong><strong>:<em> </em></strong><em> </em> The results showed that 70 (100%) of the strains in agar well diffusion method were sensitive to 4- 12 µg/ml of garlic extract with MIC 8 µg/ml. While, the results of micro dilution method showed that 40 out of 70 strains were MIC ≥ 12 µg/ml for GE.</p><p><strong><em>Conclusion</em></strong><strong>:<em> </em></strong> The results of this study indicated that the MIC and minimum bactericidal concentration of garlic extract were 8 µg/ml and 16 µg/ml, respectively. These finding indicated that garlic extract inhibit the growth of erythromycin and methicillin resistant bacterial strains.</p><p> </p></td></tr></tbody></table></div>http://jmb.tums.ac.ir/index.php/jmb/article/view/179Agar well diffusionMicro dilution methodsAllium sativum (Garlic)MethicillinErythromycinResistant |
| spellingShingle | Ramezan Ali Ataee Hassan Araqizade Rezvan Yoosefi Ali Mehrabi Tavana Mohammad Hosein Ataee Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room Journal of Medical Bacteriology Agar well diffusion Micro dilution methods Allium sativum (Garlic) Methicillin Erythromycin Resistant |
| title | Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room |
| title_full | Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room |
| title_fullStr | Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room |
| title_full_unstemmed | Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room |
| title_short | Effect of Allium sativum Extract on Erythromycin and Methicillin Resistant Bacteria Isolated from Hospital Operating Room |
| title_sort | effect of allium sativum extract on erythromycin and methicillin resistant bacteria isolated from hospital operating room |
| topic | Agar well diffusion Micro dilution methods Allium sativum (Garlic) Methicillin Erythromycin Resistant |
| url | http://jmb.tums.ac.ir/index.php/jmb/article/view/179 |
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