Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study
Oral Submucous Fibrosis (OSMF) is a chronic debilitating disease more frequently found in the South East Asian population. This disease poses a public health priority, as it is grouped under oral potentially malignant disorders, with malignant transformation rates of around 7 to 13%. Hence, early id...
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MDPI AG
2022-02-01
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| Series: | Journal of Personalized Medicine |
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| Online Access: | https://www.mdpi.com/2075-4426/12/2/208 |
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| author | Divyambika Catakapatri Venugopal Soundharya Ravindran Vidyarani Shyamsundar Sathasivasubramanian Sankarapandian Arvind Krishnamurthy Ananthi Sivagnanam Yasasve Madhavan Vijayalakshmi Ramshankar |
| author_facet | Divyambika Catakapatri Venugopal Soundharya Ravindran Vidyarani Shyamsundar Sathasivasubramanian Sankarapandian Arvind Krishnamurthy Ananthi Sivagnanam Yasasve Madhavan Vijayalakshmi Ramshankar |
| author_sort | Divyambika Catakapatri Venugopal |
| collection | DOAJ |
| description | Oral Submucous Fibrosis (OSMF) is a chronic debilitating disease more frequently found in the South East Asian population. This disease poses a public health priority, as it is grouped under oral potentially malignant disorders, with malignant transformation rates of around 7 to 13%. Hence, early identification of high-risk OSMF patients is of the utmost importance to prevent malignant transformation. Proteomic expression profiling is a promising method for identifying differentially expressed proteins for disease prognosis and risk stratification in OSMF. In this study, overexpressed proteins in OSMF, OSMF transformed into oral squamous cell carcinoma (OSCC) and normal tissues were evaluated by proteomic analysis using two-dimensional electrophoresis (2DE) and mass spectrometry, which revealed 23 upregulated proteins. Validation was done using immunohistochemistry for three secretory proteins, namely 14-3-3ε (<i>n</i> = 130), carbonic anhydrase 1 (CA 1) (<i>n</i> = 125) and heat shock protein 70 (HSP 70) (<i>n</i> = 117), which showed significant overexpression in OSMF, OSCC compared to normal. The present study is the first of its kind in India to the best of our knowledge, assessing the altered expression of proteins in OSMF and OSMF which has undergone malignant transformation, obtaining a better knowledge of the molecular pathways involved in the disease progression. The current study shows that the biomarkers studied can be potentially useful for risk stratification of OSMF to OSCC serving as novel targets for therapeutic intervention. Clinical validation of the targets can further pave way for precision medicine to improve the quality of life in OSMF patients. |
| first_indexed | 2024-03-09T21:37:27Z |
| format | Article |
| id | doaj.art-8e1f57a7728d44358094f32a2f0ec206 |
| institution | Directory Open Access Journal |
| issn | 2075-4426 |
| language | English |
| last_indexed | 2024-03-09T21:37:27Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Personalized Medicine |
| spelling | doaj.art-8e1f57a7728d44358094f32a2f0ec2062023-11-23T20:39:54ZengMDPI AGJournal of Personalized Medicine2075-44262022-02-0112220810.3390/jpm12020208Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian StudyDivyambika Catakapatri Venugopal0Soundharya Ravindran1Vidyarani Shyamsundar2Sathasivasubramanian Sankarapandian3Arvind Krishnamurthy4Ananthi Sivagnanam5Yasasve Madhavan6Vijayalakshmi Ramshankar7Department of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, IndiaDepartment of Preventive Oncology (Research), Cancer Institute (WIA), Adyar, Chennai 600020, IndiaCentre for Oral Cancer Prevention and Research, Sree Balaji Dental College and Hospital, Pallikaranai, Chennai 600100, IndiaDepartment of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, IndiaDepartment of Surgical Oncology, Cancer Institute (WIA), Adyar, Chennai 600020, IndiaDepartment of Preventive Oncology (Research), Cancer Institute (WIA), Adyar, Chennai 600020, IndiaDepartment of Oral Medicine and Radiology, Sri Ramachandra Institute of Higher Education and Research (DU), Porur, Chennai 600116, IndiaDepartment of Preventive Oncology (Research), Cancer Institute (WIA), Adyar, Chennai 600020, IndiaOral Submucous Fibrosis (OSMF) is a chronic debilitating disease more frequently found in the South East Asian population. This disease poses a public health priority, as it is grouped under oral potentially malignant disorders, with malignant transformation rates of around 7 to 13%. Hence, early identification of high-risk OSMF patients is of the utmost importance to prevent malignant transformation. Proteomic expression profiling is a promising method for identifying differentially expressed proteins for disease prognosis and risk stratification in OSMF. In this study, overexpressed proteins in OSMF, OSMF transformed into oral squamous cell carcinoma (OSCC) and normal tissues were evaluated by proteomic analysis using two-dimensional electrophoresis (2DE) and mass spectrometry, which revealed 23 upregulated proteins. Validation was done using immunohistochemistry for three secretory proteins, namely 14-3-3ε (<i>n</i> = 130), carbonic anhydrase 1 (CA 1) (<i>n</i> = 125) and heat shock protein 70 (HSP 70) (<i>n</i> = 117), which showed significant overexpression in OSMF, OSCC compared to normal. The present study is the first of its kind in India to the best of our knowledge, assessing the altered expression of proteins in OSMF and OSMF which has undergone malignant transformation, obtaining a better knowledge of the molecular pathways involved in the disease progression. The current study shows that the biomarkers studied can be potentially useful for risk stratification of OSMF to OSCC serving as novel targets for therapeutic intervention. Clinical validation of the targets can further pave way for precision medicine to improve the quality of life in OSMF patients.https://www.mdpi.com/2075-4426/12/2/208OSMFproteomicsMALDIimmunohistochemistrygene ontology |
| spellingShingle | Divyambika Catakapatri Venugopal Soundharya Ravindran Vidyarani Shyamsundar Sathasivasubramanian Sankarapandian Arvind Krishnamurthy Ananthi Sivagnanam Yasasve Madhavan Vijayalakshmi Ramshankar Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study Journal of Personalized Medicine OSMF proteomics MALDI immunohistochemistry gene ontology |
| title | Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study |
| title_full | Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study |
| title_fullStr | Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study |
| title_full_unstemmed | Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study |
| title_short | Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis—An Indian Study |
| title_sort | integrated proteomics based on 2d gel electrophoresis and mass spectrometry with validations identification of a biomarker compendium for oral submucous fibrosis an indian study |
| topic | OSMF proteomics MALDI immunohistochemistry gene ontology |
| url | https://www.mdpi.com/2075-4426/12/2/208 |
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