Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays
The immune systems of bacteria and archaea served as the inspiration for CRISPR/Cas technology, which won the 2020 Nobel Prize in Chemistry for its achievements in genetic manipulation. Because of its enormous value globally, molecular diagnostics emerged as a fresh wave of diagnostic technologies....
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Talanta Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831923000462 |
| _version_ | 1797811071035113472 |
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| author | Sophia Nazir |
| author_facet | Sophia Nazir |
| author_sort | Sophia Nazir |
| collection | DOAJ |
| description | The immune systems of bacteria and archaea served as the inspiration for CRISPR/Cas technology, which won the 2020 Nobel Prize in Chemistry for its achievements in genetic manipulation. Because of its enormous value globally, molecular diagnostics emerged as a fresh wave of diagnostic technologies. A growing number of studies have demonstrated the compatibility of CRISPR/Cas technology with biosensors and bioassays for molecular diagnostics. As highly precise, sensitive, easily programmable, and device-independent sensors, CRISPR-based detection has received a lot of interest. One of the most precise and sensitive diagnostic techniques is nucleic acid-based detection. It has the potential for detecting other biomarkers, such as proteins and tiny compounds, with more study. This study provides insight into CRISPR-based biosensors and recent advancements from nucleic acids to other non-nucleic small molecules or analytes such as proteins and presents the challenges and perspectives of CRISPR biosensors and bioassays. |
| first_indexed | 2024-03-13T07:17:12Z |
| format | Article |
| id | doaj.art-8e22e3c7e46d420db6f3874025cb1a42 |
| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-03-13T07:17:12Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj.art-8e22e3c7e46d420db6f3874025cb1a422023-06-05T04:13:21ZengElsevierTalanta Open2666-83192023-08-017100225Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassaysSophia Nazir0Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea; Department of Biotechnology, University of Science & Technology (UST), Daejeon 34141, Republic of Korea; Wolfson Nanomaterials and Devices Laboratory, School of Engineering, Computing and Mathematics, University of Plymouth, Devon, PL4 8AA, UKThe immune systems of bacteria and archaea served as the inspiration for CRISPR/Cas technology, which won the 2020 Nobel Prize in Chemistry for its achievements in genetic manipulation. Because of its enormous value globally, molecular diagnostics emerged as a fresh wave of diagnostic technologies. A growing number of studies have demonstrated the compatibility of CRISPR/Cas technology with biosensors and bioassays for molecular diagnostics. As highly precise, sensitive, easily programmable, and device-independent sensors, CRISPR-based detection has received a lot of interest. One of the most precise and sensitive diagnostic techniques is nucleic acid-based detection. It has the potential for detecting other biomarkers, such as proteins and tiny compounds, with more study. This study provides insight into CRISPR-based biosensors and recent advancements from nucleic acids to other non-nucleic small molecules or analytes such as proteins and presents the challenges and perspectives of CRISPR biosensors and bioassays.http://www.sciencedirect.com/science/article/pii/S2666831923000462CRISPRRT-PCRSARS-CoV-2ARDSSingle guide RNA (sgRNA)Biosensor |
| spellingShingle | Sophia Nazir Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays Talanta Open CRISPR RT-PCR SARS-CoV-2 ARDS Single guide RNA (sgRNA) Biosensor |
| title | Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays |
| title_full | Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays |
| title_fullStr | Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays |
| title_full_unstemmed | Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays |
| title_short | Recent progress of molecular diagnosis via CRISPR Cas-based biosensors and bioassays |
| title_sort | recent progress of molecular diagnosis via crispr cas based biosensors and bioassays |
| topic | CRISPR RT-PCR SARS-CoV-2 ARDS Single guide RNA (sgRNA) Biosensor |
| url | http://www.sciencedirect.com/science/article/pii/S2666831923000462 |
| work_keys_str_mv | AT sophianazir recentprogressofmoleculardiagnosisviacrisprcasbasedbiosensorsandbioassays |