Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis
Abstract Objective This study evaluates the probiotic activity of three vaginal Lactobacillus gasseri (H59.2, IMAUFB014, and JCM1131) and one non-vaginal L. plantarum ATCC14917 against three Candida albicans (ATCC10231, candidiasis, and healthy vaginal microbiota). Displacement of lactobacilli and a...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-07-01
|
| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13104-022-06114-z |
| _version_ | 1828427047293157376 |
|---|---|
| author | Robert Josue Rodríguez-Arias Bryan Omar Guachi-Álvarez Dominique Esther Montalvo-Vivero António Machado |
| author_facet | Robert Josue Rodríguez-Arias Bryan Omar Guachi-Álvarez Dominique Esther Montalvo-Vivero António Machado |
| author_sort | Robert Josue Rodríguez-Arias |
| collection | DOAJ |
| description | Abstract Objective This study evaluates the probiotic activity of three vaginal Lactobacillus gasseri (H59.2, IMAUFB014, and JCM1131) and one non-vaginal L. plantarum ATCC14917 against three Candida albicans (ATCC10231, candidiasis, and healthy vaginal microbiota). Displacement of lactobacilli and adhesion inhibition of C. albicans were evaluated on an abiotic surface through adhesion assays with different experimental settings (ES) through low (1.0E + 03 CFU/ml) and high (1.00E + 09 CFU/ml) levels of colonization. ES simulated dysbiosis (ES1 and ES4), candidiasis (ES2), and healthy vaginal microbiota (ES3). Results At ES2 and ES3, L. gasseri H59.2 showed discrepant inhibition values among C. albicans isolates (ES2: P = 0.008, ES3: P = 0.030; two‐way ANOVA). L. plantarum was only displaced by 23%, 31%, 54%, and 94% against low and high levels of C. albicans ATCC10231. L. plantarum was less displaced, when compared to L. gasseri strains (ES1: 61–84%, ES2: 82–96%, ES3: 83–95%, and ES4: 73–97%), showing multiple statistical differences (ES1: P = < 0.001, ES2: P = 0.003, and ES3: P = < 0.001; two‐way ANOVA). L. plantarum also showed a superior inhibition of C. albicans ATCC10231 in ES1 (81%) and ES2 (58%) when compared to L. gasseri strains (ES1: 27–73%, P < 0.001; and ES2:1–49%, P < 0.001; two‐way ANOVA). |
| first_indexed | 2024-12-10T16:55:42Z |
| format | Article |
| id | doaj.art-8e237621b1a14b9db53efc933a2f53e2 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-10T16:55:42Z |
| publishDate | 2022-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-8e237621b1a14b9db53efc933a2f53e22022-12-22T01:40:46ZengBMCBMC Research Notes1756-05002022-07-011511710.1186/s13104-022-06114-zLactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysisRobert Josue Rodríguez-Arias0Bryan Omar Guachi-Álvarez1Dominique Esther Montalvo-Vivero2António Machado3Colegio de Ciencias Biológicas y Ambientales COCIBA, Instituto de Microbiología, Laboratorio de Bacteriología, Calle Diego de Robles y Pampite, Universidad San Francisco de Quito USFQColegio de Ciencias Biológicas y Ambientales COCIBA, Instituto de Microbiología, Laboratorio de Bacteriología, Calle Diego de Robles y Pampite, Universidad San Francisco de Quito USFQColegio de Ciencias Biológicas y Ambientales COCIBA, Instituto de Microbiología, Laboratorio de Bacteriología, Calle Diego de Robles y Pampite, Universidad San Francisco de Quito USFQColegio de Ciencias Biológicas y Ambientales COCIBA, Instituto de Microbiología, Laboratorio de Bacteriología, Calle Diego de Robles y Pampite, Universidad San Francisco de Quito USFQAbstract Objective This study evaluates the probiotic activity of three vaginal Lactobacillus gasseri (H59.2, IMAUFB014, and JCM1131) and one non-vaginal L. plantarum ATCC14917 against three Candida albicans (ATCC10231, candidiasis, and healthy vaginal microbiota). Displacement of lactobacilli and adhesion inhibition of C. albicans were evaluated on an abiotic surface through adhesion assays with different experimental settings (ES) through low (1.0E + 03 CFU/ml) and high (1.00E + 09 CFU/ml) levels of colonization. ES simulated dysbiosis (ES1 and ES4), candidiasis (ES2), and healthy vaginal microbiota (ES3). Results At ES2 and ES3, L. gasseri H59.2 showed discrepant inhibition values among C. albicans isolates (ES2: P = 0.008, ES3: P = 0.030; two‐way ANOVA). L. plantarum was only displaced by 23%, 31%, 54%, and 94% against low and high levels of C. albicans ATCC10231. L. plantarum was less displaced, when compared to L. gasseri strains (ES1: 61–84%, ES2: 82–96%, ES3: 83–95%, and ES4: 73–97%), showing multiple statistical differences (ES1: P = < 0.001, ES2: P = 0.003, and ES3: P = < 0.001; two‐way ANOVA). L. plantarum also showed a superior inhibition of C. albicans ATCC10231 in ES1 (81%) and ES2 (58%) when compared to L. gasseri strains (ES1: 27–73%, P < 0.001; and ES2:1–49%, P < 0.001; two‐way ANOVA).https://doi.org/10.1186/s13104-022-06114-zCandida albicansInitial adhesionLactobacillus gasseriLactobacillus plantarumProbiotics |
| spellingShingle | Robert Josue Rodríguez-Arias Bryan Omar Guachi-Álvarez Dominique Esther Montalvo-Vivero António Machado Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis BMC Research Notes Candida albicans Initial adhesion Lactobacillus gasseri Lactobacillus plantarum Probiotics |
| title | Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis |
| title_full | Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis |
| title_fullStr | Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis |
| title_full_unstemmed | Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis |
| title_short | Lactobacilli displacement and Candida albicans inhibition on initial adhesion assays: a probiotic analysis |
| title_sort | lactobacilli displacement and candida albicans inhibition on initial adhesion assays a probiotic analysis |
| topic | Candida albicans Initial adhesion Lactobacillus gasseri Lactobacillus plantarum Probiotics |
| url | https://doi.org/10.1186/s13104-022-06114-z |
| work_keys_str_mv | AT robertjosuerodriguezarias lactobacillidisplacementandcandidaalbicansinhibitiononinitialadhesionassaysaprobioticanalysis AT bryanomarguachialvarez lactobacillidisplacementandcandidaalbicansinhibitiononinitialadhesionassaysaprobioticanalysis AT dominiqueesthermontalvovivero lactobacillidisplacementandcandidaalbicansinhibitiononinitialadhesionassaysaprobioticanalysis AT antoniomachado lactobacillidisplacementandcandidaalbicansinhibitiononinitialadhesionassaysaprobioticanalysis |