Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study
The role of the innate immune system in ovarian cancer is gaining importance. The relevance of tumor-associated macrophages (TAM) is insufficiently understood. In this pilot project, comprising the immunofluorescent staining of 30 biopsies taken from 24 patients with ovarian cancer, we evaluated the...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/2/305 |
| _version_ | 1797763091174260736 |
|---|---|
| author | Ann Vankerckhoven Roxanne Wouters Thomas Mathivet Jolien Ceusters Thaïs Baert Anaïs Van Hoylandt Holger Gerhardt Ignace Vergote An Coosemans |
| author_facet | Ann Vankerckhoven Roxanne Wouters Thomas Mathivet Jolien Ceusters Thaïs Baert Anaïs Van Hoylandt Holger Gerhardt Ignace Vergote An Coosemans |
| author_sort | Ann Vankerckhoven |
| collection | DOAJ |
| description | The role of the innate immune system in ovarian cancer is gaining importance. The relevance of tumor-associated macrophages (TAM) is insufficiently understood. In this pilot project, comprising the immunofluorescent staining of 30 biopsies taken from 24 patients with ovarian cancer, we evaluated the presence of total TAM (cluster of differentiation (CD) 68 expression), M1 (major histocompatibility complex (MHC) II expression), and M2 (anti-mannose receptor C type 1 (MRC1) expression), and the blood vessel diameter. We observed a high M1/M2 ratio in low-grade ovarian cancer compared to high-grade tumors, more total TAM and M2 in metastatic biopsies, and a further increase in total TAM and M2 at interval debulking, without beneficial effects of bevacizumab. The blood vessel diameter was indicative for M2 tumor infiltration (Spearman correlation coefficient of 0.65). These data mainly reveal an immune beneficial environment in low-grade ovarian cancer in contrast to high-grade serous ovarian cancer, where immune suppression is not altered by neoadjuvant therapy. |
| first_indexed | 2024-03-12T19:36:37Z |
| format | Article |
| id | doaj.art-8e28886361f141a2a9f7334a1fbc62f1 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T19:36:37Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8e28886361f141a2a9f7334a1fbc62f12023-08-02T04:08:22ZengMDPI AGCells2073-44092020-01-019230510.3390/cells9020305cells9020305Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot StudyAnn Vankerckhoven0Roxanne Wouters1Thomas Mathivet2Jolien Ceusters3Thaïs Baert4Anaïs Van Hoylandt5Holger Gerhardt6Ignace Vergote7An Coosemans8ImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumPARCC, HEGP Institute (team 9), INSERM U970, Université Paris Descartes, 75006 Paris, FranceImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumVascular Patterning Lab, Center for Cancer Biology, VIB, KU Leuven, 3000 Leuven, BelgiumImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumImmunOvar Research Group, Laboratory of Tumor Immunology and Immunotherapy, Department of Oncology, KU Leuven, 3000 Leuven, BelgiumThe role of the innate immune system in ovarian cancer is gaining importance. The relevance of tumor-associated macrophages (TAM) is insufficiently understood. In this pilot project, comprising the immunofluorescent staining of 30 biopsies taken from 24 patients with ovarian cancer, we evaluated the presence of total TAM (cluster of differentiation (CD) 68 expression), M1 (major histocompatibility complex (MHC) II expression), and M2 (anti-mannose receptor C type 1 (MRC1) expression), and the blood vessel diameter. We observed a high M1/M2 ratio in low-grade ovarian cancer compared to high-grade tumors, more total TAM and M2 in metastatic biopsies, and a further increase in total TAM and M2 at interval debulking, without beneficial effects of bevacizumab. The blood vessel diameter was indicative for M2 tumor infiltration (Spearman correlation coefficient of 0.65). These data mainly reveal an immune beneficial environment in low-grade ovarian cancer in contrast to high-grade serous ovarian cancer, where immune suppression is not altered by neoadjuvant therapy.https://www.mdpi.com/2073-4409/9/2/305ovarian cancermacrophagesm2 |
| spellingShingle | Ann Vankerckhoven Roxanne Wouters Thomas Mathivet Jolien Ceusters Thaïs Baert Anaïs Van Hoylandt Holger Gerhardt Ignace Vergote An Coosemans Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study Cells ovarian cancer macrophages m2 |
| title | Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study |
| title_full | Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study |
| title_fullStr | Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study |
| title_full_unstemmed | Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study |
| title_short | Opposite Macrophage Polarization in Different Subsets of Ovarian Cancer: Observation from a Pilot Study |
| title_sort | opposite macrophage polarization in different subsets of ovarian cancer observation from a pilot study |
| topic | ovarian cancer macrophages m2 |
| url | https://www.mdpi.com/2073-4409/9/2/305 |
| work_keys_str_mv | AT annvankerckhoven oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT roxannewouters oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT thomasmathivet oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT jolienceusters oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT thaisbaert oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT anaisvanhoylandt oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT holgergerhardt oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT ignacevergote oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy AT ancoosemans oppositemacrophagepolarizationindifferentsubsetsofovariancancerobservationfromapilotstudy |