Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure
Abstract Background Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-09-01
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| Series: | Clinical and Translational Allergy |
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| Online Access: | https://doi.org/10.1002/clt2.12051 |
| _version_ | 1818399864386486272 |
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| author | Juan Carlos López‐Rodríguez Juan Rodríguez‐Coira Sara Benedé Coral Barbas Domingo Barber María Teresa Villalba María Marta Escribese Alma Villaseñor Eva Batanero |
| author_facet | Juan Carlos López‐Rodríguez Juan Rodríguez‐Coira Sara Benedé Coral Barbas Domingo Barber María Teresa Villalba María Marta Escribese Alma Villaseñor Eva Batanero |
| author_sort | Juan Carlos López‐Rodríguez |
| collection | DOAJ |
| description | Abstract Background Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine‐protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development. Methods Calu‐3 cells were cultivated in air‐liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry. Results We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical‐basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L‐arginine, L‐kynurenine and L‐methionine. Conclusion This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine‐protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence. |
| first_indexed | 2024-12-14T07:27:27Z |
| format | Article |
| id | doaj.art-8e2bcd1070b0447bb4f06735abf306e1 |
| institution | Directory Open Access Journal |
| issn | 2045-7022 |
| language | English |
| last_indexed | 2024-12-14T07:27:27Z |
| publishDate | 2021-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Allergy |
| spelling | doaj.art-8e2bcd1070b0447bb4f06735abf306e12022-12-21T23:11:29ZengWileyClinical and Translational Allergy2045-70222021-09-01117n/an/a10.1002/clt2.12051Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposureJuan Carlos López‐Rodríguez0Juan Rodríguez‐Coira1Sara Benedé2Coral Barbas3Domingo Barber4María Teresa Villalba5María Marta Escribese6Alma Villaseñor7Eva Batanero8Departamento de Bioquímica y Biología Molecular Facultad de Ciencias Químicas Universidad Complutense de Madrid Madrid SpainCentro de Metabolómica y Bioanálisis (CEMBIO) Facultad de Farmacia Universidad San Pablo CEU CEU Universities Madrid SpainDepartamento de Bioquímica y Biología Molecular Facultad de Ciencias Químicas Universidad Complutense de Madrid Madrid SpainCentro de Metabolómica y Bioanálisis (CEMBIO) Facultad de Farmacia Universidad San Pablo CEU CEU Universities Madrid SpainInstituto de Medicina Molecular Aplicada (IMMA) Departamento de Ciencias Médicas Básicas Facultad de Medicina Universidad San Pablo‐CEU CEU Universities Madrid SpainDepartamento de Bioquímica y Biología Molecular Facultad de Ciencias Químicas Universidad Complutense de Madrid Madrid SpainInstituto de Medicina Molecular Aplicada (IMMA) Departamento de Ciencias Médicas Básicas Facultad de Medicina Universidad San Pablo‐CEU CEU Universities Madrid SpainCentro de Metabolómica y Bioanálisis (CEMBIO) Facultad de Farmacia Universidad San Pablo CEU CEU Universities Madrid SpainDepartamento de Bioquímica y Biología Molecular Facultad de Ciencias Químicas Universidad Complutense de Madrid Madrid SpainAbstract Background Several studies have shown a correlation between an altered metabolome and respiratory allergies. The epithelial barrier hypothesis proposes that an epithelial barrier dysfunction can result in allergic diseases development. Der p 1 allergen from house dust mite is a renowned epithelial barrier disruptor and allergy initiator due to its cysteine‐protease activity. Here, we compared the metabolic profile of the bronchial epithelium exposed or not to Der p 1 during barrier establishment to understand its active role in allergy development. Methods Calu‐3 cells were cultivated in air‐liquid interface cultures and exposed to either Der p 1 or Ole e 1 allergens during barrier establishment. The comparative metabolomics analysis of apical and basolateral media were performed using liquid chromatography and capillary electrophoresis both coupled to mass spectrometry. Results We showed that epithelial barrier disruption by Der p 1 was associated with a specific metabolic profile, which was highly dependent on the state of the epithelium at the time of contact. Moreover, an apical‐basolateral distribution of the metabolites was also observed, indicating a compartmentalization of the response with differential metabolic patterns. A number of metabolites were changed by Der p 1, mainly related to amino acids metabolism, such as L‐arginine, L‐kynurenine and L‐methionine. Conclusion This work is the first report on the metabolic response in human bronchial epithelial cells associated with cysteine‐protease Der p 1 activity, which could contribute to allergy development. Moreover, it supports a reformulated epithelial barrier hypothesis that might help to explain allergies and their increasing prevalence.https://doi.org/10.1002/clt2.12051air‐liquid interface culturebronchial epitheliumDer p 1metabolomerespiratory allergy |
| spellingShingle | Juan Carlos López‐Rodríguez Juan Rodríguez‐Coira Sara Benedé Coral Barbas Domingo Barber María Teresa Villalba María Marta Escribese Alma Villaseñor Eva Batanero Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure Clinical and Translational Allergy air‐liquid interface culture bronchial epithelium Der p 1 metabolome respiratory allergy |
| title | Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| title_full | Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| title_fullStr | Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| title_full_unstemmed | Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| title_short | Comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| title_sort | comparative metabolomics analysis of bronchial epithelium during barrier establishment after allergen exposure |
| topic | air‐liquid interface culture bronchial epithelium Der p 1 metabolome respiratory allergy |
| url | https://doi.org/10.1002/clt2.12051 |
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