Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)
<p>Abstract</p> <p>Background</p> <p>The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-05-01
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| Series: | BMC Cell Biology |
| Online Access: | http://www.biomedcentral.com/1471-2121/7/20 |
| _version_ | 1811316226180251648 |
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| author | Schneider Yves-Jacques Deloyer Patricia Zorzi Willy El Moualij Benaïssa Devillé Christelle Nollevaux Géraldine Peulen Olivier Dandrifosse Guy |
| author_facet | Schneider Yves-Jacques Deloyer Patricia Zorzi Willy El Moualij Benaïssa Devillé Christelle Nollevaux Géraldine Peulen Olivier Dandrifosse Guy |
| author_sort | Schneider Yves-Jacques |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick <it>in vivo </it>situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the characterization of the monolayer under a given experimental procedure.</p> <p>Results</p> <p>In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of <it>IAP </it>and <it>MUC5AC </it>genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. We observed that the culture conditions used (serum-free medium) did not change the phenotype of each cell type, and produced a reproducible model. The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells.</p> <p>Conclusion</p> <p>The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules.</p> |
| first_indexed | 2024-04-13T11:45:19Z |
| format | Article |
| id | doaj.art-8e2ce69702c5415da0a93e3b26df0e5e |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-04-13T11:45:19Z |
| publishDate | 2006-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-8e2ce69702c5415da0a93e3b26df0e5e2022-12-22T02:48:12ZengBMCBMC Cell Biology1471-21212006-05-01712010.1186/1471-2121-7-20Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21)Schneider Yves-JacquesDeloyer PatriciaZorzi WillyEl Moualij BenaïssaDevillé ChristelleNollevaux GéraldinePeulen OlivierDandrifosse Guy<p>Abstract</p> <p>Background</p> <p>The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick <it>in vivo </it>situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the characterization of the monolayer under a given experimental procedure.</p> <p>Results</p> <p>In this study, we investigated the activity and localization of the alkaline phosphatase and the expression of <it>IAP </it>and <it>MUC5AC </it>genes to determine a correlation between these markers and the cellular composition of a differentiated monolayer obtained from a mixture of Caco-2 and HT29-5M21 cells. We observed that the culture conditions used (serum-free medium) did not change the phenotype of each cell type, and produced a reproducible model. The alkaline phosphatase expression characterizing Caco-2 cells was influenced by the presence of HT29-5M21 cells.</p> <p>Conclusion</p> <p>The culture formed by 75% Caco-2 and 25% HT29-5M21 produce a monolayer containing the two main cell types of human intestinal epithelium and characterized by a reduced permeability to macromolecules.</p>http://www.biomedcentral.com/1471-2121/7/20 |
| spellingShingle | Schneider Yves-Jacques Deloyer Patricia Zorzi Willy El Moualij Benaïssa Devillé Christelle Nollevaux Géraldine Peulen Olivier Dandrifosse Guy Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) BMC Cell Biology |
| title | Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) |
| title_full | Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) |
| title_fullStr | Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) |
| title_full_unstemmed | Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) |
| title_short | Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21) |
| title_sort | development of a serum free co culture of human intestinal epithelium cell lines caco 2 ht29 5m21 |
| url | http://www.biomedcentral.com/1471-2121/7/20 |
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