The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming
Macrophages are the immune cells of high-immunological plasticity, which can exert both pro- and anti-inflammatory activity, as well as repolarize their phenotype to the opposite or neutral one. In this regard, M2 macrophages of the tumor-associated stroma (TAS) are a promising therapeutic target in...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/7484490 |
| _version_ | 1827079572012662784 |
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| author | Vera S. Ruzanova Svetlana S. Kirikovich Evgeniy V. Levites Anastasia S. Proskurina Evgeniya V. Dolgova Genrikh S. Ritter Yaroslav R. Efremov Tatyana D. Dubatolova Alexander V. Sysoev Danil I. Koleno Alexandr A. Ostanin Elena R. Chernykh Sergey S. Bogachev |
| author_facet | Vera S. Ruzanova Svetlana S. Kirikovich Evgeniy V. Levites Anastasia S. Proskurina Evgeniya V. Dolgova Genrikh S. Ritter Yaroslav R. Efremov Tatyana D. Dubatolova Alexander V. Sysoev Danil I. Koleno Alexandr A. Ostanin Elena R. Chernykh Sergey S. Bogachev |
| author_sort | Vera S. Ruzanova |
| collection | DOAJ |
| description | Macrophages are the immune cells of high-immunological plasticity, which can exert both pro- and anti-inflammatory activity, as well as repolarize their phenotype to the opposite or neutral one. In this regard, M2 macrophages of the tumor-associated stroma (TAS) are a promising therapeutic target in treating malignant neoplasms. Using FACS assay, we have estimated the CD11b+/Ly-6G+/Ly-6C+ fraction of macrophages from the peritoneum and TAS in intact healthy mice and those with developed Lewis carcinoma, both untreated and treated according to Karanahan technology in combination with group-specific macrophage activator (GcMAF-RF). As well, the pattern of pro- and anti-inflammatory cytokines mRNA expression in different groups of experimental and tumor-bearing animals was assessed. It was found that: (i) exposure of intact mice to GcMAF-RF results in the increased number of CD11b+/Ly-6C+ peritoneal macrophages and, at the same time, the expression pattern of cytokines in peritoneal macrophages switches from that characteristic of the mixed M1/M2 phenotype to that characteristic of the neutral M0 one; (ii) combination of Karanahan technology and GcMAF-RF treatment results in M0/M1 repolarization of TAS macrophages; (iii) in tumor-bearing mice, the response of peritoneal macrophages to such a treatment is associated with the induction of anti-inflammatory reaction, which is opposite to that in TAS macrophages. |
| first_indexed | 2024-04-25T01:44:28Z |
| format | Article |
| id | doaj.art-8e310a911b97460e8cb02b1ef53a71df |
| institution | Directory Open Access Journal |
| issn | 2314-7156 |
| language | English |
| last_indexed | 2025-03-20T02:47:41Z |
| publishDate | 2024-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj.art-8e310a911b97460e8cb02b1ef53a71df2024-10-03T07:29:40ZengHindawi LimitedJournal of Immunology Research2314-71562024-01-01202410.1155/2024/7484490The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage ReprogrammingVera S. Ruzanova0Svetlana S. Kirikovich1Evgeniy V. Levites2Anastasia S. Proskurina3Evgeniya V. Dolgova4Genrikh S. Ritter5Yaroslav R. Efremov6Tatyana D. Dubatolova7Alexander V. Sysoev8Danil I. Koleno9Alexandr A. Ostanin10Elena R. Chernykh11Sergey S. Bogachev12Institute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Cytology and GeneticsInstitute of Molecular and Cellular BiologyN.N. Vorozhtsov Novosibirsk Institute of Organic ChemistryN.N. Vorozhtsov Novosibirsk Institute of Organic ChemistryResearch Institute of Fundamental and Clinical ImmunologyResearch Institute of Fundamental and Clinical ImmunologyInstitute of Cytology and GeneticsMacrophages are the immune cells of high-immunological plasticity, which can exert both pro- and anti-inflammatory activity, as well as repolarize their phenotype to the opposite or neutral one. In this regard, M2 macrophages of the tumor-associated stroma (TAS) are a promising therapeutic target in treating malignant neoplasms. Using FACS assay, we have estimated the CD11b+/Ly-6G+/Ly-6C+ fraction of macrophages from the peritoneum and TAS in intact healthy mice and those with developed Lewis carcinoma, both untreated and treated according to Karanahan technology in combination with group-specific macrophage activator (GcMAF-RF). As well, the pattern of pro- and anti-inflammatory cytokines mRNA expression in different groups of experimental and tumor-bearing animals was assessed. It was found that: (i) exposure of intact mice to GcMAF-RF results in the increased number of CD11b+/Ly-6C+ peritoneal macrophages and, at the same time, the expression pattern of cytokines in peritoneal macrophages switches from that characteristic of the mixed M1/M2 phenotype to that characteristic of the neutral M0 one; (ii) combination of Karanahan technology and GcMAF-RF treatment results in M0/M1 repolarization of TAS macrophages; (iii) in tumor-bearing mice, the response of peritoneal macrophages to such a treatment is associated with the induction of anti-inflammatory reaction, which is opposite to that in TAS macrophages.http://dx.doi.org/10.1155/2024/7484490 |
| spellingShingle | Vera S. Ruzanova Svetlana S. Kirikovich Evgeniy V. Levites Anastasia S. Proskurina Evgeniya V. Dolgova Genrikh S. Ritter Yaroslav R. Efremov Tatyana D. Dubatolova Alexander V. Sysoev Danil I. Koleno Alexandr A. Ostanin Elena R. Chernykh Sergey S. Bogachev The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming Journal of Immunology Research |
| title | The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming |
| title_full | The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming |
| title_fullStr | The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming |
| title_full_unstemmed | The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming |
| title_short | The Macrophage Activator GcMAF-RF Enhances the Antitumor Effect of Karanahan Technology through Induction of M2–M1 Macrophage Reprogramming |
| title_sort | macrophage activator gcmaf rf enhances the antitumor effect of karanahan technology through induction of m2 m1 macrophage reprogramming |
| url | http://dx.doi.org/10.1155/2024/7484490 |
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