blaKPC gene Detection in Clinical Isolates of Carbapenem Resistant Enterobacteriaceae in a Tertiary Care Hospital
Introduction: Carbapenem resistance among Enterobacteriaceae, especially in Klebsiella pneumoniae and Escherichia coli, is an emerging problem worldwide. A common mechanism of carbapenem resistance is the production of class-A, Klebsiella pneumoniae carbapenemase (KPC). Aims and Objectives: The...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3747/24-%207759_E(C)_F(T)_PF1(PP)_PFA(NC)_P_U.pdf |
| Summary: | Introduction: Carbapenem resistance among Enterobacteriaceae,
especially in Klebsiella pneumoniae and Escherichia coli, is
an emerging problem worldwide. A common mechanism of
carbapenem resistance is the production of class-A, Klebsiella
pneumoniae carbapenemase (KPC).
Aims and Objectives: The present study focused on determining
the antibiotic resistance pattern and prevalence of bla KPC gene
coding for KPC in carbapenem resistant Enterobacteriaceae.
Methodology: Forty six carbapenem resistant isolates belonging
to the family Enterobacteriaceae were tested for antibiotic
sensitivity pattern. Modified Hodge Test (MHT) and PCR for bla
KPC gene detection were performed on these isolates. Of these,
22 were Klebsiella pneumoniae, 21 were Escherichia coli, 2 were
Citrobacter species and 1 was Proteus mirabilis
Results: Forty three (93.4%) out of the 46 isolates were resistant
to Meropenem, 34 (73.9%) were resistant to Imipenem and
30 (65.2%) were resistant to both Imipenem and Meropenem.
Modified Hodge Test was positive in 38 (82.6%) out of 46 isolates
and blaKPC gene was detected in 31 (67.4%) isolates. bla KPC gene
was detected in 28 out of the 38 MHT positive isolates. |
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| ISSN: | 2249-782X 0973-709X |