Enhanced sensitivity of neutralizing antibody detection for different AAV serotypes using HeLa cells with overexpressed AAVR

Enhanced sensitivity of neutralizing antibody detection for different AAV serotypes using HeLa cells with overexpressed AAVR

A cell-based transduction inhibition assay (TI) is widely used in clinical trials to detect neutralizing antibody (NAb) titers against recombinant adeno-associated virus (rAAV), one of the most important criteria to exclude patients in gene therapy. Different cell lines are used in cell-based TI bec...

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Bibliographic Details
Main Authors: Zhaoyue Zheng, Jingya Ye, Mi Leng, Chunmei Gan, Na Tang, Wei Li, C. Alexander Valencia, Biao Dong, Hoi Yee Chow
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-04-01
Series:Frontiers in Pharmacology
Subjects:
gene therapy
adeno-associated virus
neutralizing antibody
AAVR
stable cell line
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2023.1188290/full

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