The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria
Abstract Background The possession of immense activities of the silver nanoparticles, due to which they have played an anomalous role in the various fields of bioscience, is of big concern of study in the recent era. So, the main aim of our study was to synthesize silver nanoparticles (AgNPs) using...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-02-01
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| Series: | Bulletin of the National Research Centre |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42269-019-0057-2 |
| _version_ | 1818602006638493696 |
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| author | Shah Khalid Shah Abdul Majid Mir Ajaz Akram |
| author_facet | Shah Khalid Shah Abdul Majid Mir Ajaz Akram |
| author_sort | Shah Khalid |
| collection | DOAJ |
| description | Abstract Background The possession of immense activities of the silver nanoparticles, due to which they have played an anomalous role in the various fields of bioscience, is of big concern of study in the recent era. So, the main aim of our study was to synthesize silver nanoparticles (AgNPs) using aqueous leaf extract of Ranunculus laetus using 0.1 M silver nitrate solution, and the synthesized silver nanoparticles were tested for their antibacterial activity. Results The dark brown color of reaction mixture preliminary confirmed the synthesis of AgNPs. Further, characterization of synthesized silver nanoparticles showed UV-vis absorption peak at 442 nm, infrared spectroscopy revealed nitro compounds and aromatic amines as reducing and capping agents while X-ray diffraction revealed face-centered cubic crystallites of AgNPs with an average size of 24.125 nm. Scanning electron microscopy images confirmed spherical shape of silver nanoparticles with diameter 7.21–17.62 nm. The synthesized silver nanoparticles revealed significant antibacterial activity P < 0.05, against Escherichia coli (MTCC No. 739), Pseudomonas aeruginosa (MTCC No. 1688), Staphylococcus aureus (MTCC No. 96), and Bacillus subtilis (MTCC No. 441). The order of antibacterial potential was P. aeruginosa > S. aureus > E. coli > B. subtilis. Conclusion The ionic silver was reduced to silver nanoparticles by the action of reducing agents present in the aqueous leaf extract of Ranunculus laetus, and these nanoparticles bare credible antibacterial activity against diverse bacterial strains. |
| first_indexed | 2024-12-16T13:00:25Z |
| format | Article |
| id | doaj.art-8e33bd1074154534b86170f94ab65dae |
| institution | Directory Open Access Journal |
| issn | 2522-8307 |
| language | English |
| last_indexed | 2024-12-16T13:00:25Z |
| publishDate | 2019-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bulletin of the National Research Centre |
| spelling | doaj.art-8e33bd1074154534b86170f94ab65dae2022-12-21T22:30:51ZengSpringerOpenBulletin of the National Research Centre2522-83072019-02-014311810.1186/s42269-019-0057-2The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteriaShah Khalid0Shah Abdul Majid1Mir Ajaz Akram2Pest control and Ayurvedic Drug Research LabUniversity of KashmirBarkatullah UniversityAbstract Background The possession of immense activities of the silver nanoparticles, due to which they have played an anomalous role in the various fields of bioscience, is of big concern of study in the recent era. So, the main aim of our study was to synthesize silver nanoparticles (AgNPs) using aqueous leaf extract of Ranunculus laetus using 0.1 M silver nitrate solution, and the synthesized silver nanoparticles were tested for their antibacterial activity. Results The dark brown color of reaction mixture preliminary confirmed the synthesis of AgNPs. Further, characterization of synthesized silver nanoparticles showed UV-vis absorption peak at 442 nm, infrared spectroscopy revealed nitro compounds and aromatic amines as reducing and capping agents while X-ray diffraction revealed face-centered cubic crystallites of AgNPs with an average size of 24.125 nm. Scanning electron microscopy images confirmed spherical shape of silver nanoparticles with diameter 7.21–17.62 nm. The synthesized silver nanoparticles revealed significant antibacterial activity P < 0.05, against Escherichia coli (MTCC No. 739), Pseudomonas aeruginosa (MTCC No. 1688), Staphylococcus aureus (MTCC No. 96), and Bacillus subtilis (MTCC No. 441). The order of antibacterial potential was P. aeruginosa > S. aureus > E. coli > B. subtilis. Conclusion The ionic silver was reduced to silver nanoparticles by the action of reducing agents present in the aqueous leaf extract of Ranunculus laetus, and these nanoparticles bare credible antibacterial activity against diverse bacterial strains.http://link.springer.com/article/10.1186/s42269-019-0057-2Silver nanoparticlesRanunculus laetusUV-visX-ray diffractionScanning electron microscopyAntibacterial activity |
| spellingShingle | Shah Khalid Shah Abdul Majid Mir Ajaz Akram The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria Bulletin of the National Research Centre Silver nanoparticles Ranunculus laetus UV-vis X-ray diffraction Scanning electron microscopy Antibacterial activity |
| title | The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria |
| title_full | The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria |
| title_fullStr | The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria |
| title_full_unstemmed | The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria |
| title_short | The prophylactic effect of Ranunculus laetus (Wall)-mediated silver nanoparticles against some Gram-positive and Gram-negative bacteria |
| title_sort | prophylactic effect of ranunculus laetus wall mediated silver nanoparticles against some gram positive and gram negative bacteria |
| topic | Silver nanoparticles Ranunculus laetus UV-vis X-ray diffraction Scanning electron microscopy Antibacterial activity |
| url | http://link.springer.com/article/10.1186/s42269-019-0057-2 |
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