Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques
Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of p...
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MDPI AG
2021-06-01
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| Series: | Infectious Disease Reports |
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| Online Access: | https://www.mdpi.com/2036-7449/13/2/49 |
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| author | Peuli Nath Md Alamgir Kabir Somaiyeh Khoubafarin Doust Aniruddha Ray |
| author_facet | Peuli Nath Md Alamgir Kabir Somaiyeh Khoubafarin Doust Aniruddha Ray |
| author_sort | Peuli Nath |
| collection | DOAJ |
| description | Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages. |
| first_indexed | 2024-03-10T10:46:57Z |
| format | Article |
| id | doaj.art-8e34a6726e1c4f2ca616f1ab748e3c6c |
| institution | Directory Open Access Journal |
| issn | 2036-7449 |
| language | English |
| last_indexed | 2024-03-10T10:46:57Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Infectious Disease Reports |
| spelling | doaj.art-8e34a6726e1c4f2ca616f1ab748e3c6c2023-11-21T22:33:46ZengMDPI AGInfectious Disease Reports2036-74492021-06-0113251853910.3390/idr13020049Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care TechniquesPeuli Nath0Md Alamgir Kabir1Somaiyeh Khoubafarin Doust2Aniruddha Ray3Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USADepartment of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USADepartment of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USADepartment of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USAHerpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.https://www.mdpi.com/2036-7449/13/2/49herpes simplex virusdetectiondiagnosticspoint-of-care devicesmicrofluidicsimaging and microscopy |
| spellingShingle | Peuli Nath Md Alamgir Kabir Somaiyeh Khoubafarin Doust Aniruddha Ray Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques Infectious Disease Reports herpes simplex virus detection diagnostics point-of-care devices microfluidics imaging and microscopy |
| title | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
| title_full | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
| title_fullStr | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
| title_full_unstemmed | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
| title_short | Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques |
| title_sort | diagnosis of herpes simplex virus laboratory and point of care techniques |
| topic | herpes simplex virus detection diagnostics point-of-care devices microfluidics imaging and microscopy |
| url | https://www.mdpi.com/2036-7449/13/2/49 |
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