Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein
In plant cells, the interconversion of formyl- and methylene-tetrahydrofolates is catalyzed by a bifunctional protein possessing methenyltetrallydrofolate cydohydrolase (EC 3.5.4.9) and methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities. The present work reports the isolation and sequen...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
1999-11-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.1999.10.4.171 |
| _version_ | 1818731983043297280 |
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| author | Chen Liangfu Nargang Frank E. Cossin Edwin A. |
| author_facet | Chen Liangfu Nargang Frank E. Cossin Edwin A. |
| author_sort | Chen Liangfu |
| collection | DOAJ |
| description | In plant cells, the interconversion of formyl- and methylene-tetrahydrofolates is catalyzed by a bifunctional protein possessing methenyltetrallydrofolate cydohydrolase (EC 3.5.4.9) and methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities. The present work reports the isolation and sequencing of a cDNA that encodes this protein. Polydonal antibodies, raised against purified pea cytosolic dehydrogenase:cyclohydrolase, were used to screen a λgt 11 cDNA expression library, constructed from leaf extracts of this species. The screen identified a phage containing a cDNA insert with an open reading frame encoding a 294 amino acid protein (Mr 31,344). The deduced primary structure of this protein contained most of the conserved regions found in other dehydrogenase:cyclohydrolase proteins including the corresponding domains of the trifunctional C1-THF synthases of mammalian and yeast origins. |
| first_indexed | 2024-12-17T23:26:20Z |
| format | Article |
| id | doaj.art-8e35176358c8440cad03058d3494b2fc |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-17T23:26:20Z |
| publishDate | 1999-11-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-8e35176358c8440cad03058d3494b2fc2022-12-21T21:28:45ZengDe GruyterPteridines0933-48072195-47201999-11-0110417117710.1515/pteridines.1999.10.4.171Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase ProteinChen Liangfu0Nargang Frank E.1Cossin Edwin A.2Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, CanadaDepartment of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, CanadaIn plant cells, the interconversion of formyl- and methylene-tetrahydrofolates is catalyzed by a bifunctional protein possessing methenyltetrallydrofolate cydohydrolase (EC 3.5.4.9) and methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5) activities. The present work reports the isolation and sequencing of a cDNA that encodes this protein. Polydonal antibodies, raised against purified pea cytosolic dehydrogenase:cyclohydrolase, were used to screen a λgt 11 cDNA expression library, constructed from leaf extracts of this species. The screen identified a phage containing a cDNA insert with an open reading frame encoding a 294 amino acid protein (Mr 31,344). The deduced primary structure of this protein contained most of the conserved regions found in other dehydrogenase:cyclohydrolase proteins including the corresponding domains of the trifunctional C1-THF synthases of mammalian and yeast origins.https://doi.org/10.1515/pteridines.1999.10.4.171cdnamethylenetetrahydrofolate dehydrogenasemethenyltetrahydrofolate cyclohydrolasebifunctional proteinpisum satwum l |
| spellingShingle | Chen Liangfu Nargang Frank E. Cossin Edwin A. Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein Pteridines cdna methylenetetrahydrofolate dehydrogenase methenyltetrahydrofolate cyclohydrolase bifunctional protein pisum satwum l |
| title | Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein |
| title_full | Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein |
| title_fullStr | Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein |
| title_full_unstemmed | Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein |
| title_short | Isolation and Sequencing of a Plant cDNA Encoding a Bifunctional Methylenetetrahydrofolate Dehydrogenase : Methenyltetrahydrofolate Cyclohydrolase Protein |
| title_sort | isolation and sequencing of a plant cdna encoding a bifunctional methylenetetrahydrofolate dehydrogenase methenyltetrahydrofolate cyclohydrolase protein |
| topic | cdna methylenetetrahydrofolate dehydrogenase methenyltetrahydrofolate cyclohydrolase bifunctional protein pisum satwum l |
| url | https://doi.org/10.1515/pteridines.1999.10.4.171 |
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