Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats
Shang Gao,1 Chenmoji Wang,1 Lijie Qi,2 Songlin Liang,1 Xintian Qu,1 Wei Liu,3,* Nianhu Li4,* 1The First Clinical Medical College of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China; 2Qilu Hospital of Shandong University, Jinan,...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2024-01-01
|
| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/bushen-huoxue-formula-inhibits-il-1-induced-apoptosis-and-extracellula-peer-reviewed-fulltext-article-JIR |
| _version_ | 1827388007077904384 |
|---|---|
| author | Gao S Wang C Qi L Liang S Qu X Liu W Li N |
| author_facet | Gao S Wang C Qi L Liang S Qu X Liu W Li N |
| author_sort | Gao S |
| collection | DOAJ |
| description | Shang Gao,1 Chenmoji Wang,1 Lijie Qi,2 Songlin Liang,1 Xintian Qu,1 Wei Liu,3,* Nianhu Li4,* 1The First Clinical Medical College of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China; 2Qilu Hospital of Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China; 4Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Nianhu Li; Wei Liu, Email tigerlee073@126.com; weiliu800109@163.comBackground: The method of action of Bushen Formula (BSHXF) in the treatment of intervertebral disc degeneration (IVDD) was uncovered in this work using in vivo and in vitro tests. To clarify the mechanism of action of BSHXF, we validated the rat intervertebral disc degeneration model and the nucleus pulposus cell degeneration model.Methods: In an in vivo model of IVDD the study explores the impact of BSHXF on mitochondrial function, pro-inflammatory cytokines, pro-apoptotic factors, and matrix metalloproteinases. Additionally, it evaluates the induced degeneration of nucleus pulposus (NP) cells in an in vitro model stimulated by interleukin-1 β (IL-1β). The study measures the effects of BSHXF on both the inflammatory response and mitochondrial function.Results: The MRI results showed that BSHXF reduced intervertebral disc volume reduction and degradation of NP tissue. HE, SO-FG and immunofluorescence further confirmed the protective effect of BSHXF on degenerative intervertebral discs. BSHXF reduced the inflammatory levels of IL-6 IL-1β and TNF-α in degenerative intervertebral disc tissue. Meanwhile, JC-1, mPTP and ROS detection revealed that BSHXF can restore mitochondrial function by regulating the expression of antioxidant proteins, playing a protective role in NP cells. Finally, the WB results showed that BSHXF can alleviate IL-1β mediate the degeneration of NP cells. BSHXF can alleviate NP cell apoptosis by inhibiting the expression of bax, cleaved caspase-3, caspase-3, and cyt-c, and increasing the expression of Bcl-2.Conclusion: This study reveals that BSHXF inhibits the development of inflammatory factors, which may play a significant role in intervertebral disc degeneration. This implies that BSHXF is a suitable herbal medication for future research into inflammatory cytokine treatment.Keywords: intervertebral disc degeneration, nucleus pulposus cell, BSHXF, apoptosis, inflammatory |
| first_indexed | 2024-03-08T16:10:35Z |
| format | Article |
| id | doaj.art-8e3aea5d109748a694c1213cb011f6ba |
| institution | Directory Open Access Journal |
| issn | 1178-7031 |
| language | English |
| last_indexed | 2024-03-08T16:10:35Z |
| publishDate | 2024-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj.art-8e3aea5d109748a694c1213cb011f6ba2024-01-07T18:06:43ZengDove Medical PressJournal of Inflammation Research1178-70312024-01-01Volume 1712113689530Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in RatsGao SWang CQi LLiang SQu XLiu WLi NShang Gao,1 Chenmoji Wang,1 Lijie Qi,2 Songlin Liang,1 Xintian Qu,1 Wei Liu,3,* Nianhu Li4,* 1The First Clinical Medical College of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China; 2Qilu Hospital of Shandong University, Jinan, Shandong Province, People’s Republic of China; 3Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China; 4Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, People’s Republic of China*These authors contributed equally to this workCorrespondence: Nianhu Li; Wei Liu, Email tigerlee073@126.com; weiliu800109@163.comBackground: The method of action of Bushen Formula (BSHXF) in the treatment of intervertebral disc degeneration (IVDD) was uncovered in this work using in vivo and in vitro tests. To clarify the mechanism of action of BSHXF, we validated the rat intervertebral disc degeneration model and the nucleus pulposus cell degeneration model.Methods: In an in vivo model of IVDD the study explores the impact of BSHXF on mitochondrial function, pro-inflammatory cytokines, pro-apoptotic factors, and matrix metalloproteinases. Additionally, it evaluates the induced degeneration of nucleus pulposus (NP) cells in an in vitro model stimulated by interleukin-1 β (IL-1β). The study measures the effects of BSHXF on both the inflammatory response and mitochondrial function.Results: The MRI results showed that BSHXF reduced intervertebral disc volume reduction and degradation of NP tissue. HE, SO-FG and immunofluorescence further confirmed the protective effect of BSHXF on degenerative intervertebral discs. BSHXF reduced the inflammatory levels of IL-6 IL-1β and TNF-α in degenerative intervertebral disc tissue. Meanwhile, JC-1, mPTP and ROS detection revealed that BSHXF can restore mitochondrial function by regulating the expression of antioxidant proteins, playing a protective role in NP cells. Finally, the WB results showed that BSHXF can alleviate IL-1β mediate the degeneration of NP cells. BSHXF can alleviate NP cell apoptosis by inhibiting the expression of bax, cleaved caspase-3, caspase-3, and cyt-c, and increasing the expression of Bcl-2.Conclusion: This study reveals that BSHXF inhibits the development of inflammatory factors, which may play a significant role in intervertebral disc degeneration. This implies that BSHXF is a suitable herbal medication for future research into inflammatory cytokine treatment.Keywords: intervertebral disc degeneration, nucleus pulposus cell, BSHXF, apoptosis, inflammatoryhttps://www.dovepress.com/bushen-huoxue-formula-inhibits-il-1-induced-apoptosis-and-extracellula-peer-reviewed-fulltext-article-JIRintervertebral disc degenerationnucleus pulposus cellbshxfapoptosisinflammatory |
| spellingShingle | Gao S Wang C Qi L Liang S Qu X Liu W Li N Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats Journal of Inflammation Research intervertebral disc degeneration nucleus pulposus cell bshxf apoptosis inflammatory |
| title | Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats |
| title_full | Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats |
| title_fullStr | Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats |
| title_full_unstemmed | Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats |
| title_short | Bushen Huoxue Formula Inhibits IL-1β-Induced Apoptosis and Extracellular Matrix Degradation in the Nucleus Pulposus Cells and Improves Intervertebral Disc Degeneration in Rats |
| title_sort | bushen huoxue formula inhibits il 1 beta induced apoptosis and extracellular matrix degradation in the nucleus pulposus cells and improves intervertebral disc degeneration in rats |
| topic | intervertebral disc degeneration nucleus pulposus cell bshxf apoptosis inflammatory |
| url | https://www.dovepress.com/bushen-huoxue-formula-inhibits-il-1-induced-apoptosis-and-extracellula-peer-reviewed-fulltext-article-JIR |
| work_keys_str_mv | AT gaos bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT wangc bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT qil bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT liangs bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT qux bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT liuw bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats AT lin bushenhuoxueformulainhibitsil1betainducedapoptosisandextracellularmatrixdegradationinthenucleuspulposuscellsandimprovesintervertebraldiscdegenerationinrats |