Using 3D printed eggs to examine the egg-rejection behaviour of wild birds
The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laboriou...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2015-05-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/965.pdf |
| _version_ | 1797418814899486720 |
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| author | Branislav Igic Valerie Nunez Henning U. Voss Rebecca Croston Zachary Aidala Analía V. López Aimee Van Tatenhove Mandë E. Holford Matthew D. Shawkey Mark E. Hauber |
| author_facet | Branislav Igic Valerie Nunez Henning U. Voss Rebecca Croston Zachary Aidala Analía V. López Aimee Van Tatenhove Mandë E. Holford Matthew D. Shawkey Mark E. Hauber |
| author_sort | Branislav Igic |
| collection | DOAJ |
| description | The coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and prone to human error. As an alternative, 3D printing may reduce human error, enable more precise manipulation of egg size and shape, and provide a more accurate and replicable protocol for generating artificial stimuli than traditional methods. However, the usefulness of 3D printing technology for egg rejection research remains to be tested. Here, we applied 3D printing technology to the extensively studied egg rejection behaviour of American robins, Turdus migratorius. Eggs of the robin’s brood parasites, brown-headed cowbirds, Molothrus ater, vary greatly in size and shape, but it is unknown whether host egg rejection decisions differ across this gradient of natural variation. We printed artificial eggs that encompass the natural range of shapes and sizes of cowbird eggs, painted them to resemble either robin or cowbird egg colour, and used them to artificially parasitize nests of breeding wild robins. In line with previous studies, we show that robins accept mimetically coloured and reject non-mimetically coloured artificial eggs. Although we found no evidence that subtle differences in parasitic egg size or shape affect robins’ rejection decisions, 3D printing will provide an opportunity for more extensive experimentation on the potential biological or evolutionary significance of size and shape variation of foreign eggs in rejection decisions. We provide a detailed protocol for generating 3D printed eggs using either personal 3D printers or commercial printing services, and highlight additional potential future applications for this technology in the study of egg rejection. |
| first_indexed | 2024-03-09T06:38:22Z |
| format | Article |
| id | doaj.art-8e3bcd4e591c4ddaa250940e04db8f10 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:38:22Z |
| publishDate | 2015-05-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8e3bcd4e591c4ddaa250940e04db8f102023-12-03T10:54:16ZengPeerJ Inc.PeerJ2167-83592015-05-013e96510.7717/peerj.965965Using 3D printed eggs to examine the egg-rejection behaviour of wild birdsBranislav Igic0Valerie Nunez1Henning U. Voss2Rebecca Croston3Zachary Aidala4Analía V. López5Aimee Van Tatenhove6Mandë E. Holford7Matthew D. Shawkey8Mark E. Hauber9Department of Biology, University of Akron, Akron, OH, USADepartment of Psychology, Hunter College, and The Graduate Center of the City University of New York, NY, USACitigroup Biomedical Imaging Center, Weill Cornell Medical College, New York, NY, USADepartment of Biology, University of Nevada—Reno, Reno, NV, USADepartment of Psychology, Hunter College, and The Graduate Center of the City University of New York, NY, USADepartamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, ArgentinaDepartment of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY, USADepartment of Chemistry, Hunter College, The Graduate Center of the City University of New York, and The American Museum of Natural History, NY, USADepartment of Biology, University of Akron, Akron, OH, USADepartment of Psychology, Hunter College, and The Graduate Center of the City University of New York, NY, USAThe coevolutionary relationships between brood parasites and their hosts are often studied by examining the egg rejection behaviour of host species using artificial eggs. However, the traditional methods for producing artificial eggs out of plasticine, plastic, wood, or plaster-of-Paris are laborious, imprecise, and prone to human error. As an alternative, 3D printing may reduce human error, enable more precise manipulation of egg size and shape, and provide a more accurate and replicable protocol for generating artificial stimuli than traditional methods. However, the usefulness of 3D printing technology for egg rejection research remains to be tested. Here, we applied 3D printing technology to the extensively studied egg rejection behaviour of American robins, Turdus migratorius. Eggs of the robin’s brood parasites, brown-headed cowbirds, Molothrus ater, vary greatly in size and shape, but it is unknown whether host egg rejection decisions differ across this gradient of natural variation. We printed artificial eggs that encompass the natural range of shapes and sizes of cowbird eggs, painted them to resemble either robin or cowbird egg colour, and used them to artificially parasitize nests of breeding wild robins. In line with previous studies, we show that robins accept mimetically coloured and reject non-mimetically coloured artificial eggs. Although we found no evidence that subtle differences in parasitic egg size or shape affect robins’ rejection decisions, 3D printing will provide an opportunity for more extensive experimentation on the potential biological or evolutionary significance of size and shape variation of foreign eggs in rejection decisions. We provide a detailed protocol for generating 3D printed eggs using either personal 3D printers or commercial printing services, and highlight additional potential future applications for this technology in the study of egg rejection.https://peerj.com/articles/965.pdfArtificial egg Turdus migratorius Brood parasitism3D printingEgg rejectionAmerican robin |
| spellingShingle | Branislav Igic Valerie Nunez Henning U. Voss Rebecca Croston Zachary Aidala Analía V. López Aimee Van Tatenhove Mandë E. Holford Matthew D. Shawkey Mark E. Hauber Using 3D printed eggs to examine the egg-rejection behaviour of wild birds PeerJ Artificial egg Turdus migratorius Brood parasitism 3D printing Egg rejection American robin |
| title | Using 3D printed eggs to examine the egg-rejection behaviour of wild birds |
| title_full | Using 3D printed eggs to examine the egg-rejection behaviour of wild birds |
| title_fullStr | Using 3D printed eggs to examine the egg-rejection behaviour of wild birds |
| title_full_unstemmed | Using 3D printed eggs to examine the egg-rejection behaviour of wild birds |
| title_short | Using 3D printed eggs to examine the egg-rejection behaviour of wild birds |
| title_sort | using 3d printed eggs to examine the egg rejection behaviour of wild birds |
| topic | Artificial egg Turdus migratorius Brood parasitism 3D printing Egg rejection American robin |
| url | https://peerj.com/articles/965.pdf |
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