Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds
Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species <i>...
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MDPI AG
2021-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/8/1496 |
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| author | Sohyun Bae Jae Woo Han Quang Le Dang Hun Kim Gyung Ja Choi |
| author_facet | Sohyun Bae Jae Woo Han Quang Le Dang Hun Kim Gyung Ja Choi |
| author_sort | Sohyun Bae |
| collection | DOAJ |
| description | Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species <i>Platycladus orientalis</i> suppressed the disease development of rice blast caused by <i>Magnaporthe oryzae</i>. Through a series of chromatography procedures in combination with activity-guided fractionation, we isolated and identified a total of eleven compounds including four labdane-type diterpenes (<b>1</b>–<b>4</b>), six isopimarane-type diterpenes (<b>5</b>–<b>10</b>), and one sesquiterpene (<b>11</b>). Of the identified compounds, the MIC values of compounds <b>1</b>, <b>2</b>, <b>5</b> & <b>6</b> mixture, <b>9</b>, and <b>11</b> ranged from 100 to 200 μg/mL against <i>M. oryzae</i>, whereas the other compounds were over 200 μg/mL. When rice plants were treated with the antifungal compounds, compounds <b>1</b>, <b>2</b>, and <b>9</b> effectively suppressed the development of rice blast at all concentrations tested by over 75% compared to the non-treatment control. In addition, a mixture of compounds <b>5</b> & <b>6</b> that constituted 66% of the <i>P. orientalis</i> ethyl acetate fraction also exhibited a moderate disease control efficacy. Together, our data suggest that the methanol extract of <i>P. orientalis</i> including terpenoid compounds has potential as a crop protection agent. |
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| language | English |
| last_indexed | 2024-03-10T08:28:28Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-8e4142d4a28a47fe8bbae0f21dd4ec8e2023-11-22T09:17:06ZengMDPI AGPlants2223-77472021-07-01108149610.3390/plants10081496Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal CompoundsSohyun Bae0Jae Woo Han1Quang Le Dang2Hun Kim3Gyung Ja Choi4Center for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaDepartment of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, KoreaResearch and Development Center of Bioactive Compounds, Vietnam Institute of Industrial Chemistry, Hanoi 100000, VietnamCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaCenter for Eco-Friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaPlants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species <i>Platycladus orientalis</i> suppressed the disease development of rice blast caused by <i>Magnaporthe oryzae</i>. Through a series of chromatography procedures in combination with activity-guided fractionation, we isolated and identified a total of eleven compounds including four labdane-type diterpenes (<b>1</b>–<b>4</b>), six isopimarane-type diterpenes (<b>5</b>–<b>10</b>), and one sesquiterpene (<b>11</b>). Of the identified compounds, the MIC values of compounds <b>1</b>, <b>2</b>, <b>5</b> & <b>6</b> mixture, <b>9</b>, and <b>11</b> ranged from 100 to 200 μg/mL against <i>M. oryzae</i>, whereas the other compounds were over 200 μg/mL. When rice plants were treated with the antifungal compounds, compounds <b>1</b>, <b>2</b>, and <b>9</b> effectively suppressed the development of rice blast at all concentrations tested by over 75% compared to the non-treatment control. In addition, a mixture of compounds <b>5</b> & <b>6</b> that constituted 66% of the <i>P. orientalis</i> ethyl acetate fraction also exhibited a moderate disease control efficacy. Together, our data suggest that the methanol extract of <i>P. orientalis</i> including terpenoid compounds has potential as a crop protection agent.https://www.mdpi.com/2223-7747/10/8/1496<i>Platycladus orientalis</i>terpeneantifungal activityplant disease control |
| spellingShingle | Sohyun Bae Jae Woo Han Quang Le Dang Hun Kim Gyung Ja Choi Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds Plants <i>Platycladus orientalis</i> terpene antifungal activity plant disease control |
| title | Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds |
| title_full | Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds |
| title_fullStr | Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds |
| title_full_unstemmed | Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds |
| title_short | Plant Disease Control Efficacy of <i>Platycladus orientalis</i> and Its Antifungal Compounds |
| title_sort | plant disease control efficacy of i platycladus orientalis i and its antifungal compounds |
| topic | <i>Platycladus orientalis</i> terpene antifungal activity plant disease control |
| url | https://www.mdpi.com/2223-7747/10/8/1496 |
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