Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria
MLO (mildew locus O) genes play a vital role in plant disease defense system, especially powdery mildew (PM). Lagenaria siceraria is a distinct Cucurbitaceae crop, and PM is one of the most serious diseases threatening crop production and quality. Although MLOs have been exploited in many Cucurbitac...
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Elsevier
2023-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023018315 |
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| author | Jian Wang Xiaohua Wu Ying Wang Xinyi Wu Baogen Wang Zhongfu Lu Guojing Li |
| author_facet | Jian Wang Xiaohua Wu Ying Wang Xinyi Wu Baogen Wang Zhongfu Lu Guojing Li |
| author_sort | Jian Wang |
| collection | DOAJ |
| description | MLO (mildew locus O) genes play a vital role in plant disease defense system, especially powdery mildew (PM). Lagenaria siceraria is a distinct Cucurbitaceae crop, and PM is one of the most serious diseases threatening crop production and quality. Although MLOs have been exploited in many Cucurbitaceae species, genome-wide mining of MLO gene family in bottle gourd has not been surveyed yet. Here we identified 16 MLO genes in our recently assembled L. siceraria genome. A total of 343 unique MLO protein sequences from 20 species were characterized and compared to deduce a generally high level of purifying selection and the occurrence of regions related to candidate susceptibility factors in the evolutional divergence. LsMLOs were clustered in six clades containing seven conserved transmembrane domains and 10 clade-specific motifs along with deletion and variation. Three genes (LsMLO3, LsMLO6, and LsMLO13) in clade V showed high sequence identity with orthologues involved in PM susceptibility. The expression pattern of LsMLOs was tissue-specific but not cultivar-specific. Furthermore, it was indicated by qRT-PCR and RNA-seq that LsMLO3 and LsMLO13 were highly upregulated in response to PM stress. Subsequent sequence analysis revealed the structural deletion of LsMLO13 and a single nonsynonymous substitution of LsMLO3 in the PM-resistant genotype. Taken all together, it is speculated that LsMLO13 is likely a major PM susceptibility factor. The results of this study provide new insights into MLO family genes in bottle gourd and find a potential candidate S gene for PM tolerance breeding. |
| first_indexed | 2024-04-09T15:19:28Z |
| format | Article |
| id | doaj.art-8e43d62b51544eda890c54268ab4feca |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T15:19:28Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj.art-8e43d62b51544eda890c54268ab4feca2023-04-29T14:50:24ZengElsevierHeliyon2405-84402023-04-0194e14624Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria sicerariaJian Wang0Xiaohua Wu1Ying Wang2Xinyi Wu3Baogen Wang4Zhongfu Lu5Guojing Li6Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaInstitute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaCorresponding author.; Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaMLO (mildew locus O) genes play a vital role in plant disease defense system, especially powdery mildew (PM). Lagenaria siceraria is a distinct Cucurbitaceae crop, and PM is one of the most serious diseases threatening crop production and quality. Although MLOs have been exploited in many Cucurbitaceae species, genome-wide mining of MLO gene family in bottle gourd has not been surveyed yet. Here we identified 16 MLO genes in our recently assembled L. siceraria genome. A total of 343 unique MLO protein sequences from 20 species were characterized and compared to deduce a generally high level of purifying selection and the occurrence of regions related to candidate susceptibility factors in the evolutional divergence. LsMLOs were clustered in six clades containing seven conserved transmembrane domains and 10 clade-specific motifs along with deletion and variation. Three genes (LsMLO3, LsMLO6, and LsMLO13) in clade V showed high sequence identity with orthologues involved in PM susceptibility. The expression pattern of LsMLOs was tissue-specific but not cultivar-specific. Furthermore, it was indicated by qRT-PCR and RNA-seq that LsMLO3 and LsMLO13 were highly upregulated in response to PM stress. Subsequent sequence analysis revealed the structural deletion of LsMLO13 and a single nonsynonymous substitution of LsMLO3 in the PM-resistant genotype. Taken all together, it is speculated that LsMLO13 is likely a major PM susceptibility factor. The results of this study provide new insights into MLO family genes in bottle gourd and find a potential candidate S gene for PM tolerance breeding.http://www.sciencedirect.com/science/article/pii/S2405844023018315Bottle gourdMLO familyPhylogenetic analysisPowdery mildewExpression analysis |
| spellingShingle | Jian Wang Xiaohua Wu Ying Wang Xinyi Wu Baogen Wang Zhongfu Lu Guojing Li Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria Heliyon Bottle gourd MLO family Phylogenetic analysis Powdery mildew Expression analysis |
| title | Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria |
| title_full | Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria |
| title_fullStr | Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria |
| title_full_unstemmed | Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria |
| title_short | Genome-wide characterization and expression analysis of the MLO gene family sheds light on powdery mildew resistance in Lagenaria siceraria |
| title_sort | genome wide characterization and expression analysis of the mlo gene family sheds light on powdery mildew resistance in lagenaria siceraria |
| topic | Bottle gourd MLO family Phylogenetic analysis Powdery mildew Expression analysis |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023018315 |
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