Validation of reference genes for gene expression studies in tartary buckwheat (Fagopyrum tataricum Gaertn.) using quantitative real-time PCR

Validation of reference genes for gene expression studies in tartary buckwheat (Fagopyrum tataricum Gaertn.) using quantitative real-time PCR

Quantitative real-time reverse transcriptase polymerase chain reaction is a sensitive technique for quantifying gene expression levels. By implementing three distinct algorithms (geNorm, normFinder and BestKeeper), we have validated the stability of the expression of seven candidate reference genes...

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Bibliographic Details
Main Authors: Chenglei Li, Haixia Zhao, Maofei Li, Panfeng Yao, Qingqing Li, Xuerong Zhao, Anhu Wang, Hui Chen, Zizhong Tang, Tongliang Bu, Qi Wu
Format: Article
Language:English
Published: PeerJ Inc. 2019-02-01
Series:PeerJ
Subjects:
Tartary buckwheat
Quantitative real-time PCR
Reference genes
Online Access:https://peerj.com/articles/6522.pdf

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