The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro
Background/Aims: This study focused on investigating the expression of several inflammatory cytokines and chemokines, including regulatory proteins in the astrocytes of mice stimulated with IL-17. Materials and Methods: The cultured astrocytes from the spinal cords of mice were stimulated with IL-17...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2016-03-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/443060 |
| _version_ | 1818050330652311552 |
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| author | Shaohui Zong Keke Li Gaofeng Zeng Ye Fang Jingmin Zhao |
| author_facet | Shaohui Zong Keke Li Gaofeng Zeng Ye Fang Jingmin Zhao |
| author_sort | Shaohui Zong |
| collection | DOAJ |
| description | Background/Aims: This study focused on investigating the expression of several inflammatory cytokines and chemokines, including regulatory proteins in the astrocytes of mice stimulated with IL-17. Materials and Methods: The cultured astrocytes from the spinal cords of mice were stimulated with IL-17. The expression of interleukin-6 (IL-6), tumor necrosis factor (TNF), monocyte chemotactic protein-1/5 (MCP-1/5) and macrophage inflammatory protein-2 (MIP-2) stimulated with IL-17 (50 ng/ml) at different time points (3 h, 6 h, 12 h, 24 h and 48 h) were determined using real-time PCR and ELISA. The expressions of A20 (tumor necrosis factor a inducible protein 3, TNFAIP3) and NF-κB were examined using real-time PCR and western blotting. Results: Compared with the control group, the mRNA expression levels of IL-6, TNF, MCP-1/5 and MIP-2 increased significantly at 6 h after IL-17 stimulation, while the protein expression levels also increased significantly and peaked at 12 h. The mRNA expression level of NF-κB increased and peaked at 6 h before gradually declining, while the expression of A20 decreased. The protein expression level of NF-κB increased and peaked at 12 h, while the expression A20 had an opposite response. Conclusion: The study showed that NF-κB may have an effect on the cytokines secreted by astrocytes after IL-17 stimulation. Moreover, both A20 and NF-κB could regulate the expression and secretion of inflammatory mediators. |
| first_indexed | 2024-12-10T10:51:46Z |
| format | Article |
| id | doaj.art-8e47e3aceff048d09cf095e84c97ec98 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T10:51:46Z |
| publishDate | 2016-03-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-8e47e3aceff048d09cf095e84c97ec982022-12-22T01:52:00ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-03-013831100111010.1159/000443060443060The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In VitroShaohui ZongKeke LiGaofeng ZengYe FangJingmin ZhaoBackground/Aims: This study focused on investigating the expression of several inflammatory cytokines and chemokines, including regulatory proteins in the astrocytes of mice stimulated with IL-17. Materials and Methods: The cultured astrocytes from the spinal cords of mice were stimulated with IL-17. The expression of interleukin-6 (IL-6), tumor necrosis factor (TNF), monocyte chemotactic protein-1/5 (MCP-1/5) and macrophage inflammatory protein-2 (MIP-2) stimulated with IL-17 (50 ng/ml) at different time points (3 h, 6 h, 12 h, 24 h and 48 h) were determined using real-time PCR and ELISA. The expressions of A20 (tumor necrosis factor a inducible protein 3, TNFAIP3) and NF-κB were examined using real-time PCR and western blotting. Results: Compared with the control group, the mRNA expression levels of IL-6, TNF, MCP-1/5 and MIP-2 increased significantly at 6 h after IL-17 stimulation, while the protein expression levels also increased significantly and peaked at 12 h. The mRNA expression level of NF-κB increased and peaked at 6 h before gradually declining, while the expression of A20 decreased. The protein expression level of NF-κB increased and peaked at 12 h, while the expression A20 had an opposite response. Conclusion: The study showed that NF-κB may have an effect on the cytokines secreted by astrocytes after IL-17 stimulation. Moreover, both A20 and NF-κB could regulate the expression and secretion of inflammatory mediators.http://www.karger.com/Article/FullText/443060Interleukin-17AstrocyteInflammatory cytokinesChemokinesA20 |
| spellingShingle | Shaohui Zong Keke Li Gaofeng Zeng Ye Fang Jingmin Zhao The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro Cellular Physiology and Biochemistry Interleukin-17 Astrocyte Inflammatory cytokines Chemokines A20 |
| title | The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro |
| title_full | The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro |
| title_fullStr | The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro |
| title_full_unstemmed | The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro |
| title_short | The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro |
| title_sort | effects of interleukin 17 il 17 related inflammatory cytokines and a20 regulatory proteins on astrocytes in spinal cord cultured in vitro |
| topic | Interleukin-17 Astrocyte Inflammatory cytokines Chemokines A20 |
| url | http://www.karger.com/Article/FullText/443060 |
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