SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses
Abstract Stem cell therapy is a promising strategy to rescue visual impairment caused by retinal degeneration. Previous studies have proposed controversial theories about whether in situ retinal stem cells (RSCs) are present in adult human eye tissue. Single‐cell RNA sequencing (scRNA‐seq) has emerg...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | MedComm |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mco2.198 |
| _version_ | 1811166339943890944 |
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| author | Xiaotang Wang Wei Fan Zongren Xu Qi Zhang Na Li Ruonan Li Guoqing Wang Siyuan He Wanqian Li Dan Liao Zhi Zhang Nan Shu Jiaxing Huang Chenyang Zhao Shengping Hou |
| author_facet | Xiaotang Wang Wei Fan Zongren Xu Qi Zhang Na Li Ruonan Li Guoqing Wang Siyuan He Wanqian Li Dan Liao Zhi Zhang Nan Shu Jiaxing Huang Chenyang Zhao Shengping Hou |
| author_sort | Xiaotang Wang |
| collection | DOAJ |
| description | Abstract Stem cell therapy is a promising strategy to rescue visual impairment caused by retinal degeneration. Previous studies have proposed controversial theories about whether in situ retinal stem cells (RSCs) are present in adult human eye tissue. Single‐cell RNA sequencing (scRNA‐seq) has emerged as one of the most powerful tools to reveal the heterogeneity of tissue cells. By using scRNA‐seq, we explored the cell heterogeneity of different subregions of adult human eyes, including pars plicata, pars plana, retinal pigment epithelium (RPE), iris, and neural retina (NR). We identified one subpopulation expressing SRY‐box transcription factor 2 (SOX2) as RSCs, which were present in the pars plicata of the adult human eye. Further analysis showed the identified subpopulation of RSCs expressed specific markers aquaporin 1 (AQP1) and tetraspanin 12 (TSPAN12). We, therefore, isolated this subpopulation using these two markers by flow sorting and found that the isolated RSCs could proliferate and differentiate into some retinal cell types, including photoreceptors, neurons, RPE cells, microglia, astrocytes, horizontal cells, bipolar cells, and ganglion cells; whereas, AQP1− TSPAN12− cells did not have this differentiation potential. In conclusion, our results showed that SOX2‐positive RSCs are present in the pars plicata and may be valuable for treating human retinal diseases due to their proliferation and differentiation potential. |
| first_indexed | 2024-04-10T15:50:49Z |
| format | Article |
| id | doaj.art-8e4817675269478889352424604ed336 |
| institution | Directory Open Access Journal |
| issn | 2688-2663 |
| language | English |
| last_indexed | 2024-04-10T15:50:49Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | MedComm |
| spelling | doaj.art-8e4817675269478889352424604ed3362023-02-11T14:06:29ZengWileyMedComm2688-26632023-02-0141n/an/a10.1002/mco2.198SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analysesXiaotang Wang0Wei Fan1Zongren Xu2Qi Zhang3Na Li4Ruonan Li5Guoqing Wang6Siyuan He7Wanqian Li8Dan Liao9Zhi Zhang10Nan Shu11Jiaxing Huang12Chenyang Zhao13Shengping Hou14The First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaDepartment of Biochemistry and Molecular Biology College of Basic Medicine Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaThe First Affiliated Hospital of Chongqing Medical University Chongqing ChinaAbstract Stem cell therapy is a promising strategy to rescue visual impairment caused by retinal degeneration. Previous studies have proposed controversial theories about whether in situ retinal stem cells (RSCs) are present in adult human eye tissue. Single‐cell RNA sequencing (scRNA‐seq) has emerged as one of the most powerful tools to reveal the heterogeneity of tissue cells. By using scRNA‐seq, we explored the cell heterogeneity of different subregions of adult human eyes, including pars plicata, pars plana, retinal pigment epithelium (RPE), iris, and neural retina (NR). We identified one subpopulation expressing SRY‐box transcription factor 2 (SOX2) as RSCs, which were present in the pars plicata of the adult human eye. Further analysis showed the identified subpopulation of RSCs expressed specific markers aquaporin 1 (AQP1) and tetraspanin 12 (TSPAN12). We, therefore, isolated this subpopulation using these two markers by flow sorting and found that the isolated RSCs could proliferate and differentiate into some retinal cell types, including photoreceptors, neurons, RPE cells, microglia, astrocytes, horizontal cells, bipolar cells, and ganglion cells; whereas, AQP1− TSPAN12− cells did not have this differentiation potential. In conclusion, our results showed that SOX2‐positive RSCs are present in the pars plicata and may be valuable for treating human retinal diseases due to their proliferation and differentiation potential.https://doi.org/10.1002/mco2.198retinal degenerationretinal stem cellscRNA‐seqSOX2+AQP1+TSPAN12+ |
| spellingShingle | Xiaotang Wang Wei Fan Zongren Xu Qi Zhang Na Li Ruonan Li Guoqing Wang Siyuan He Wanqian Li Dan Liao Zhi Zhang Nan Shu Jiaxing Huang Chenyang Zhao Shengping Hou SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses MedComm retinal degeneration retinal stem cell scRNA‐seq SOX2+AQP1+TSPAN12+ |
| title | SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses |
| title_full | SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses |
| title_fullStr | SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses |
| title_full_unstemmed | SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses |
| title_short | SOX2‐positive retinal stem cells are identified in adult human pars plicata by single‐cell transcriptomic analyses |
| title_sort | sox2 positive retinal stem cells are identified in adult human pars plicata by single cell transcriptomic analyses |
| topic | retinal degeneration retinal stem cell scRNA‐seq SOX2+AQP1+TSPAN12+ |
| url | https://doi.org/10.1002/mco2.198 |
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