In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata
The ascomycete fungus Alternaria alternata causes early blight, one of economically the most important tomato diseases. Due to frequent use of fungicides, A. alternata has developed resistance with negative economic and environmental consequences. Research of new ways to control fungal pathogens has...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-04-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/aiht-2022-73-3620 |
| _version_ | 1818139857936973824 |
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| author | Martinko Katarina Ivanković Siniša Đermić Edyta Đermić Damir |
| author_facet | Martinko Katarina Ivanković Siniša Đermić Edyta Đermić Damir |
| author_sort | Martinko Katarina |
| collection | DOAJ |
| description | The ascomycete fungus Alternaria alternata causes early blight, one of economically the most important tomato diseases. Due to frequent use of fungicides, A. alternata has developed resistance with negative economic and environmental consequences. Research of new ways to control fungal pathogens has turned its eye to environmentally friendly chemicals with low toxicity such as boronic acids. The aim of our study was therefore to test the antifungal effects of phenylboronic and boric acid in vitro on A. alternata. We isolated the pathogen from a symptomatic tomato plant and determined the minimum inhibitory concentration of phenylboronic and boric acid on A. alternata mycelial growth using the poisoned food technique. The antifungal effect was tested on a wide range of phenylboronic and boric acid concentrations (from 0.04 % to 0.3 %) applied separately to agar with mycelial disc of the pathogen. After five days of incubation, phenylboronic acid at low concentration (0.05 %) completely inhibited mycelial growth. Boric acid, in turn, did not significantly slow down mycelial growth but did reduce sporulation and confirmed its fungistatic effect. Our findings point to the potential use of phenylboronic acid to control phytopathogenic fungi. This is, to our knowledge, the first report on its antifungal effect on an agriculturally important pathogen in vitro. Moreover, since A. alternata is also a human pathogen, these results may have clinical ramifications. |
| first_indexed | 2024-12-11T10:34:46Z |
| format | Article |
| id | doaj.art-8e4b14556b5b4768914f8364f436026e |
| institution | Directory Open Access Journal |
| issn | 1848-6312 |
| language | English |
| last_indexed | 2024-12-11T10:34:46Z |
| publishDate | 2022-04-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj.art-8e4b14556b5b4768914f8364f436026e2022-12-22T01:10:44ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122022-04-01731838710.2478/aiht-2022-73-3620In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternataMartinko Katarina0Ivanković Siniša1Đermić Edyta2Đermić Damir3University of Zagreb Faculty of Agriculture, Division of Phytomedicine, Zagreb, CroatiaRuđer Bošković Institute, Division of Molecular Medicine, Zagreb, CroatiaUniversity of Zagreb Faculty of Agriculture, Division of Phytomedicine, Zagreb, CroatiaRuđer Bošković Institute, Division of Molecular Biology, Zagreb, CroatiaThe ascomycete fungus Alternaria alternata causes early blight, one of economically the most important tomato diseases. Due to frequent use of fungicides, A. alternata has developed resistance with negative economic and environmental consequences. Research of new ways to control fungal pathogens has turned its eye to environmentally friendly chemicals with low toxicity such as boronic acids. The aim of our study was therefore to test the antifungal effects of phenylboronic and boric acid in vitro on A. alternata. We isolated the pathogen from a symptomatic tomato plant and determined the minimum inhibitory concentration of phenylboronic and boric acid on A. alternata mycelial growth using the poisoned food technique. The antifungal effect was tested on a wide range of phenylboronic and boric acid concentrations (from 0.04 % to 0.3 %) applied separately to agar with mycelial disc of the pathogen. After five days of incubation, phenylboronic acid at low concentration (0.05 %) completely inhibited mycelial growth. Boric acid, in turn, did not significantly slow down mycelial growth but did reduce sporulation and confirmed its fungistatic effect. Our findings point to the potential use of phenylboronic acid to control phytopathogenic fungi. This is, to our knowledge, the first report on its antifungal effect on an agriculturally important pathogen in vitro. Moreover, since A. alternata is also a human pathogen, these results may have clinical ramifications.https://doi.org/10.2478/aiht-2022-73-3620boronic acidsearly blightminimal inhibitory concentrationmycelial growthsporulationtomatoboronske kiselinekoncentrična pjegavostminimalna inhibitorna koncentracija rajčicasporulacija |
| spellingShingle | Martinko Katarina Ivanković Siniša Đermić Edyta Đermić Damir In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata Arhiv za Higijenu Rada i Toksikologiju boronic acids early blight minimal inhibitory concentration mycelial growth sporulation tomato boronske kiseline koncentrična pjegavost minimalna inhibitorna koncentracija rajčica sporulacija |
| title | In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata |
| title_full | In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata |
| title_fullStr | In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata |
| title_full_unstemmed | In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata |
| title_short | In vitro antifungal effect of phenylboronic and boric acid on Alternaria alternata |
| title_sort | in vitro antifungal effect of phenylboronic and boric acid on alternaria alternata |
| topic | boronic acids early blight minimal inhibitory concentration mycelial growth sporulation tomato boronske kiseline koncentrična pjegavost minimalna inhibitorna koncentracija rajčica sporulacija |
| url | https://doi.org/10.2478/aiht-2022-73-3620 |
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