Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro
Abstract Aim The accumulation of amyloid beta (Aβ) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Aβ has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether ne...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-12-01
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| Series: | Neuropsychopharmacology Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/npr2.12074 |
| _version_ | 1811177945328254976 |
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| author | Rena Kono Gyu Li Kim Hidetaka Nagata Yuji Ikegaya Ryuta Koyama |
| author_facet | Rena Kono Gyu Li Kim Hidetaka Nagata Yuji Ikegaya Ryuta Koyama |
| author_sort | Rena Kono |
| collection | DOAJ |
| description | Abstract Aim The accumulation of amyloid beta (Aβ) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Aβ has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether neuronal activity affects Aβ‐induced synapse loss remains unknown. Therefore, we addressed this question using a primary culture of hippocampal neurons. Method The neuronal activity of cultured hippocampal neurons from mouse pups was increased using the chemogenetic technique designer receptors exclusively activated by designer drugs (DREADD). The cultured neurons were treated with Aβ, and synapse density was assessed by immunocytochemistry. Results Aβ decreased the synapse density probably by decreasing postsynapse. On the other hand, enhanced neuronal activity did not affect the synapse density significantly. However, there was a trend that enhanced neuronal activity increased especially presynapse density. Conclusion We found that enhanced neuronal activity did not affect Aβ‐induced synapse loss in vitro. |
| first_indexed | 2024-04-11T06:09:51Z |
| format | Article |
| id | doaj.art-8e4b56ef5bff4ecba44891652ead05ca |
| institution | Directory Open Access Journal |
| issn | 2574-173X |
| language | English |
| last_indexed | 2024-04-11T06:09:51Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neuropsychopharmacology Reports |
| spelling | doaj.art-8e4b56ef5bff4ecba44891652ead05ca2022-12-22T04:41:19ZengWileyNeuropsychopharmacology Reports2574-173X2019-12-0139430631110.1002/npr2.12074Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitroRena Kono0Gyu Li Kim1Hidetaka Nagata2Yuji Ikegaya3Ryuta Koyama4Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences The University of Tokyo Tokyo JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences The University of Tokyo Tokyo JapanPlatform Technology Research Unit Sumitomo Dainippon Pharma Co., Ltd. Osaka JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences The University of Tokyo Tokyo JapanLaboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences The University of Tokyo Tokyo JapanAbstract Aim The accumulation of amyloid beta (Aβ) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Aβ has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether neuronal activity affects Aβ‐induced synapse loss remains unknown. Therefore, we addressed this question using a primary culture of hippocampal neurons. Method The neuronal activity of cultured hippocampal neurons from mouse pups was increased using the chemogenetic technique designer receptors exclusively activated by designer drugs (DREADD). The cultured neurons were treated with Aβ, and synapse density was assessed by immunocytochemistry. Results Aβ decreased the synapse density probably by decreasing postsynapse. On the other hand, enhanced neuronal activity did not affect the synapse density significantly. However, there was a trend that enhanced neuronal activity increased especially presynapse density. Conclusion We found that enhanced neuronal activity did not affect Aβ‐induced synapse loss in vitro.https://doi.org/10.1002/npr2.12074Alzheimer's diseaseamyloid betahippocampusneuronal activitysynapse |
| spellingShingle | Rena Kono Gyu Li Kim Hidetaka Nagata Yuji Ikegaya Ryuta Koyama Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro Neuropsychopharmacology Reports Alzheimer's disease amyloid beta hippocampus neuronal activity synapse |
| title | Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro |
| title_full | Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro |
| title_fullStr | Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro |
| title_full_unstemmed | Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro |
| title_short | Induced neuronal activity does not attenuate amyloid beta‐induced synaptic loss in vitro |
| title_sort | induced neuronal activity does not attenuate amyloid beta induced synaptic loss in vitro |
| topic | Alzheimer's disease amyloid beta hippocampus neuronal activity synapse |
| url | https://doi.org/10.1002/npr2.12074 |
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