The effect of long-term cigarette smoking on selected skin barrier proteins and lipids
Abstract The negative impact of cigarette smoking on the skin includes accelerated aging, pigmentation disorders, and impaired wound healing, but its effect on the skin barrier is not completely understood. Here, we studied the changes in selected epidermal proteins and lipids between smokers (45–66...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38178-7 |
| _version_ | 1827895291627438080 |
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| author | Kristýna Hergesell Anna Paraskevopoulou Lukáš Opálka Vladimír Velebný Kateřina Vávrová Iva Dolečková |
| author_facet | Kristýna Hergesell Anna Paraskevopoulou Lukáš Opálka Vladimír Velebný Kateřina Vávrová Iva Dolečková |
| author_sort | Kristýna Hergesell |
| collection | DOAJ |
| description | Abstract The negative impact of cigarette smoking on the skin includes accelerated aging, pigmentation disorders, and impaired wound healing, but its effect on the skin barrier is not completely understood. Here, we studied the changes in selected epidermal proteins and lipids between smokers (45–66 years, smoking > 10 years, > 10 cigarettes per day) and non-smokers. Volar forearm epidermal and stratum corneum samples, obtained by suction blister and tape stripping, respectively, showed increased thickness in smokers. In the epidermis of smokers, we observed a significant upregulation of filaggrin, loricrin, and a trend of increased involucrin but no differences were found in the case of transglutaminase 1 and kallikrein-related peptidase 7, on the gene and protein levels. No significant changes were observed in the major skin barrier lipids, except for increased cholesterol sulfate in smokers. Liquid chromatography coupled with mass spectrometry revealed shorter acyl chains in ceramides, and an increased proportion of sphingosine and 6-hydroxysphingosine ceramides (with C4 trans-double bond) over dihydrosphingosine and phytosphingosine ceramides in smokers, suggesting altered desaturase 1 activity. Smokers had more ordered lipid chains found by infrared spectroscopy. In conclusion, cigarette smoking perturbs the homeostasis of the barrier proteins and lipids even at a site not directly exposed to smoke. |
| first_indexed | 2024-03-12T22:18:03Z |
| format | Article |
| id | doaj.art-8e4c4ef10093417780237198a177108d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T22:18:03Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8e4c4ef10093417780237198a177108d2023-07-23T11:12:14ZengNature PortfolioScientific Reports2045-23222023-07-011311810.1038/s41598-023-38178-7The effect of long-term cigarette smoking on selected skin barrier proteins and lipidsKristýna Hergesell0Anna Paraskevopoulou1Lukáš Opálka2Vladimír Velebný3Kateřina Vávrová4Iva Dolečková5Skin Barrier Research Group, Faculty of Pharmacy, Charles UniversitySkin Barrier Research Group, Faculty of Pharmacy, Charles UniversitySkin Barrier Research Group, Faculty of Pharmacy, Charles UniversityContipro a.s.Skin Barrier Research Group, Faculty of Pharmacy, Charles UniversityContipro a.s.Abstract The negative impact of cigarette smoking on the skin includes accelerated aging, pigmentation disorders, and impaired wound healing, but its effect on the skin barrier is not completely understood. Here, we studied the changes in selected epidermal proteins and lipids between smokers (45–66 years, smoking > 10 years, > 10 cigarettes per day) and non-smokers. Volar forearm epidermal and stratum corneum samples, obtained by suction blister and tape stripping, respectively, showed increased thickness in smokers. In the epidermis of smokers, we observed a significant upregulation of filaggrin, loricrin, and a trend of increased involucrin but no differences were found in the case of transglutaminase 1 and kallikrein-related peptidase 7, on the gene and protein levels. No significant changes were observed in the major skin barrier lipids, except for increased cholesterol sulfate in smokers. Liquid chromatography coupled with mass spectrometry revealed shorter acyl chains in ceramides, and an increased proportion of sphingosine and 6-hydroxysphingosine ceramides (with C4 trans-double bond) over dihydrosphingosine and phytosphingosine ceramides in smokers, suggesting altered desaturase 1 activity. Smokers had more ordered lipid chains found by infrared spectroscopy. In conclusion, cigarette smoking perturbs the homeostasis of the barrier proteins and lipids even at a site not directly exposed to smoke.https://doi.org/10.1038/s41598-023-38178-7 |
| spellingShingle | Kristýna Hergesell Anna Paraskevopoulou Lukáš Opálka Vladimír Velebný Kateřina Vávrová Iva Dolečková The effect of long-term cigarette smoking on selected skin barrier proteins and lipids Scientific Reports |
| title | The effect of long-term cigarette smoking on selected skin barrier proteins and lipids |
| title_full | The effect of long-term cigarette smoking on selected skin barrier proteins and lipids |
| title_fullStr | The effect of long-term cigarette smoking on selected skin barrier proteins and lipids |
| title_full_unstemmed | The effect of long-term cigarette smoking on selected skin barrier proteins and lipids |
| title_short | The effect of long-term cigarette smoking on selected skin barrier proteins and lipids |
| title_sort | effect of long term cigarette smoking on selected skin barrier proteins and lipids |
| url | https://doi.org/10.1038/s41598-023-38178-7 |
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