Computational Modeling of the Electrochemical System of Lipase Activity Detection
This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5’-ferrocenylpen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2008-06-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/6/3873/ |
| _version_ | 1798035324580921344 |
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| author | Valdemaras Razumas Gintaras ValinÄÂius Ilja Ignatjev Feliksas Ivanauskas Mantas Puida |
| author_facet | Valdemaras Razumas Gintaras ValinÄÂius Ilja Ignatjev Feliksas Ivanauskas Mantas Puida |
| author_sort | Valdemaras Razumas |
| collection | DOAJ |
| description | This paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5’-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process. |
| first_indexed | 2024-04-11T20:56:39Z |
| format | Article |
| id | doaj.art-8e4d30523173463e806f68fc0f74520d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T20:56:39Z |
| publishDate | 2008-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8e4d30523173463e806f68fc0f74520d2022-12-22T04:03:40ZengMDPI AGSensors1424-82202008-06-018638733879Computational Modeling of the Electrochemical System of Lipase Activity DetectionValdemaras RazumasGintaras ValinÄÂiusIlja IgnatjevFeliksas IvanauskasMantas PuidaThis paper presents computational modeling of response kinetics of bioelectroanalytical system based on solid supported lipase substrate and lipase interaction. The model assumes that lipase substrate is formed by dripping and drying a small amount of the ethanol solution of 9-(5’-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two mathematical models have been developed and evaluated trough computational simulation series by comparing them to experimental data. The results of simulation demonstrate that a good fitting might be obtained only taking into account non-linear substrate wash off process.http://www.mdpi.com/1424-8220/8/6/3873/Amperometric biosensorlipase activityferrocene-based substratemodelingsimulation |
| spellingShingle | Valdemaras Razumas Gintaras ValinÄÂius Ilja Ignatjev Feliksas Ivanauskas Mantas Puida Computational Modeling of the Electrochemical System of Lipase Activity Detection Sensors Amperometric biosensor lipase activity ferrocene-based substrate modeling simulation |
| title | Computational Modeling of the Electrochemical System of Lipase Activity Detection |
| title_full | Computational Modeling of the Electrochemical System of Lipase Activity Detection |
| title_fullStr | Computational Modeling of the Electrochemical System of Lipase Activity Detection |
| title_full_unstemmed | Computational Modeling of the Electrochemical System of Lipase Activity Detection |
| title_short | Computational Modeling of the Electrochemical System of Lipase Activity Detection |
| title_sort | computational modeling of the electrochemical system of lipase activity detection |
| topic | Amperometric biosensor lipase activity ferrocene-based substrate modeling simulation |
| url | http://www.mdpi.com/1424-8220/8/6/3873/ |
| work_keys_str_mv | AT valdemarasrazumas computationalmodelingoftheelectrochemicalsystemoflipaseactivitydetection AT gintarasvalinaaius computationalmodelingoftheelectrochemicalsystemoflipaseactivitydetection AT iljaignatjev computationalmodelingoftheelectrochemicalsystemoflipaseactivitydetection AT feliksasivanauskas computationalmodelingoftheelectrochemicalsystemoflipaseactivitydetection AT mantaspuida computationalmodelingoftheelectrochemicalsystemoflipaseactivitydetection |