High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis
Neuronal damage in bacterial meningitis (BM) partly stems from the host´s inflammatory response and induced oxidative stress (OS). We studied the association of cerebrospinal fluid (CSF) biomarkers indicating oxidative damage to proteins with course of illness and outcome in childhood BM in Angola....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/621 |
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| author | Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Manuel Leite Cruzeiro Máximo Vento Tuula Pelkonen |
| author_facet | Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Manuel Leite Cruzeiro Máximo Vento Tuula Pelkonen |
| author_sort | Emilie Rugemalira |
| collection | DOAJ |
| description | Neuronal damage in bacterial meningitis (BM) partly stems from the host´s inflammatory response and induced oxidative stress (OS). We studied the association of cerebrospinal fluid (CSF) biomarkers indicating oxidative damage to proteins with course of illness and outcome in childhood BM in Angola. Ortho-tyrosine/phenylalanine (o-Tyr/Phe), 3-chlorotyrosine/para-tyrosine (3Cl-Tyr/p-Tyr), and 3-nitrotyrosine/para-tyrosine (3NO<sub>2</sub>-Tyr/p-Tyr) concentration ratios were measured in 79 BM admission CSF samples, employing liquid chromatography coupled to tandem mass spectrometry. Besides death, disease outcomes were registered on Day 7 of treatment and one month after discharge (control visit). The outcome was graded according to the modified Glasgow Outcome Scale (GOS), which considers neurological and audiological sequelae. Children with a o-Tyr/Phe ratio below the median were more likely to present focal convulsions and secondary fever during recovery and suboptimal outcome (GOS < 5) on Day 7 and at control visit (odds ratio (OR) 2.85; 95% CI 1.14–7.14 and OR 5.23; 95% CI 1.66–16.52, respectively). Their most common sequela was ataxia on Day 7 and at control visit (OR 8.55; 95% CI 2.27–32.22 and OR 5.83; 95% CI 1.12–30.4, respectively). The association of a higher admission CSF o-Tyr/Phe ratio with a better course and outcome for pediatric BM points to a beneficial effect of OS. |
| first_indexed | 2024-03-11T07:00:42Z |
| format | Article |
| id | doaj.art-8e55282493254ea3968cbe1bf7c18824 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:00:42Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-8e55282493254ea3968cbe1bf7c188242023-11-17T09:17:24ZengMDPI AGAntioxidants2076-39212023-03-0112362110.3390/antiox12030621High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial MeningitisEmilie Rugemalira0Irmeli Roine1Julia Kuligowski2Ángel Sánchez-Illana3José David Piñeiro-Ramos4Sture Andersson5Manuel Leite Cruzeiro6Máximo Vento7Tuula Pelkonen8Children’s Hospital, Pediatric Research Center, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandFaculty of Medicine, University Diego Portales, Avenida Manuel Rodríguez Sur 343, Santiago 8370109, ChileHealth Research Institute La Fe (IISLAFE), Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainHealth Research Institute La Fe (IISLAFE), Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainHealth Research Institute La Fe (IISLAFE), Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainChildren’s Hospital, Pediatric Research Center, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandHospital Pediátrico David Bernardino, Rua Amilcar Cabral, Luanda, AngolaHealth Research Institute La Fe (IISLAFE), Avenida Fernando Abril Martorell 106, 46026 Valencia, SpainChildren’s Hospital, Pediatric Research Center, Helsinki University Hospital, Stenbäckinkatu 9, 00029 Helsinki, FinlandNeuronal damage in bacterial meningitis (BM) partly stems from the host´s inflammatory response and induced oxidative stress (OS). We studied the association of cerebrospinal fluid (CSF) biomarkers indicating oxidative damage to proteins with course of illness and outcome in childhood BM in Angola. Ortho-tyrosine/phenylalanine (o-Tyr/Phe), 3-chlorotyrosine/para-tyrosine (3Cl-Tyr/p-Tyr), and 3-nitrotyrosine/para-tyrosine (3NO<sub>2</sub>-Tyr/p-Tyr) concentration ratios were measured in 79 BM admission CSF samples, employing liquid chromatography coupled to tandem mass spectrometry. Besides death, disease outcomes were registered on Day 7 of treatment and one month after discharge (control visit). The outcome was graded according to the modified Glasgow Outcome Scale (GOS), which considers neurological and audiological sequelae. Children with a o-Tyr/Phe ratio below the median were more likely to present focal convulsions and secondary fever during recovery and suboptimal outcome (GOS < 5) on Day 7 and at control visit (odds ratio (OR) 2.85; 95% CI 1.14–7.14 and OR 5.23; 95% CI 1.66–16.52, respectively). Their most common sequela was ataxia on Day 7 and at control visit (OR 8.55; 95% CI 2.27–32.22 and OR 5.83; 95% CI 1.12–30.4, respectively). The association of a higher admission CSF o-Tyr/Phe ratio with a better course and outcome for pediatric BM points to a beneficial effect of OS.https://www.mdpi.com/2076-3921/12/3/621oxidative stressprotein oxidationbiomarkerortho-tyrosinechildren |
| spellingShingle | Emilie Rugemalira Irmeli Roine Julia Kuligowski Ángel Sánchez-Illana José David Piñeiro-Ramos Sture Andersson Manuel Leite Cruzeiro Máximo Vento Tuula Pelkonen High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis Antioxidants oxidative stress protein oxidation biomarker ortho-tyrosine children |
| title | High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis |
| title_full | High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis |
| title_fullStr | High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis |
| title_full_unstemmed | High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis |
| title_short | High Concentration of Protein Oxidation Biomarker O-Tyr/Phe Predicts Better Outcome in Childhood Bacterial Meningitis |
| title_sort | high concentration of protein oxidation biomarker o tyr phe predicts better outcome in childhood bacterial meningitis |
| topic | oxidative stress protein oxidation biomarker ortho-tyrosine children |
| url | https://www.mdpi.com/2076-3921/12/3/621 |
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