Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations
Graphene oxide’s (GO) intravascular applications and biocompatibility are not fully explored yet, although it has been proposed as an anticancer drug transporter, antibacterial factor or component of wearable devices. Bivalent cations and the number of particles’ atom layers, as well as their struct...
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MDPI AG
2023-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/10/2495 |
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| author | Marcin Z. Krasoń Anna Paradowska Martyna Fronczek Mateusz Lejawa Natalia Kamieńska Michał Krejca Anna Kolanowska Sławomir Boncel Marek W. Radomski |
| author_facet | Marcin Z. Krasoń Anna Paradowska Martyna Fronczek Mateusz Lejawa Natalia Kamieńska Michał Krejca Anna Kolanowska Sławomir Boncel Marek W. Radomski |
| author_sort | Marcin Z. Krasoń |
| collection | DOAJ |
| description | Graphene oxide’s (GO) intravascular applications and biocompatibility are not fully explored yet, although it has been proposed as an anticancer drug transporter, antibacterial factor or component of wearable devices. Bivalent cations and the number of particles’ atom layers, as well as their structural oxygen content and pH of the dispersion, all affect the GO size, shape, dispersibility and biological effects. Bovine serum albumin (BSA), an important blood plasma protein, is expected to improve GO dispersion stability in physiological concentrations of the precipitating calcium and magnesium cations to enable effective and safe tissue perfusion. Methods: Four types of GO commercially available aqueous dispersions (with different particle structures) were diluted, sonicated and studied in the presence of BSA and physiological cation concentrations. Nanoparticle populations sizes, electrical conductivity, zeta potential (Zetasizer NanoZS), structure (TEM and CryoTEM), functional groups content (micro titration) and dispersion pH were analyzed in consecutive preparation stages. Results: BSA effectively prevented the aggregation of GO in precipitating concentrations of physiological bivalent cations. The final polydispersity indexes were reduced from 0.66–0.91 to 0.36–0.43. The GO-containing isotonic dispersions were stable with the following Z-ave results: GO1 421.1 nm, GO2 382.6 nm, GO3 440.2 nm and GO4 490.1 nm. The GO behavior was structure-dependent. Conclusion: BSA effectively stabilized four types of GO dispersions in an isotonic dispersion containing aggregating bivalent physiological cations. |
| first_indexed | 2024-03-10T20:57:44Z |
| format | Article |
| id | doaj.art-8e584edb6ef04f6aa844fcbcedc559fe |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T20:57:44Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8e584edb6ef04f6aa844fcbcedc559fe2023-11-19T17:45:20ZengMDPI AGPharmaceutics1999-49232023-10-011510249510.3390/pharmaceutics15102495Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent CationsMarcin Z. Krasoń0Anna Paradowska1Martyna Fronczek2Mateusz Lejawa3Natalia Kamieńska4Michał Krejca5Anna Kolanowska6Sławomir Boncel7Marek W. Radomski8Silesian Park of Medical Technology Kardio-Med Silesia, 41-800 Zabrze, PolandSilesian Park of Medical Technology Kardio-Med Silesia, 41-800 Zabrze, PolandSilesian Park of Medical Technology Kardio-Med Silesia, 41-800 Zabrze, PolandSilesian Park of Medical Technology Kardio-Med Silesia, 41-800 Zabrze, PolandDepartment of Cardiac, Vascular and Endovascular Surgery and Transplantology, Medical University of Silesia in Katowice, Silesian Center for Heart Diseases in Zabrze, 41-800 Zabrze, PolandCardiac Surgery Department, Medical University of Łódź, 90-419 Łódź, PolandDepartment of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, PolandDepartment of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Faculty of Chemistry, Silesian University of Technology, 44-100 Gliwice, PolandDepartment of Anatomy, Physiology and Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, SK S7N 5E5, CanadaGraphene oxide’s (GO) intravascular applications and biocompatibility are not fully explored yet, although it has been proposed as an anticancer drug transporter, antibacterial factor or component of wearable devices. Bivalent cations and the number of particles’ atom layers, as well as their structural oxygen content and pH of the dispersion, all affect the GO size, shape, dispersibility and biological effects. Bovine serum albumin (BSA), an important blood plasma protein, is expected to improve GO dispersion stability in physiological concentrations of the precipitating calcium and magnesium cations to enable effective and safe tissue perfusion. Methods: Four types of GO commercially available aqueous dispersions (with different particle structures) were diluted, sonicated and studied in the presence of BSA and physiological cation concentrations. Nanoparticle populations sizes, electrical conductivity, zeta potential (Zetasizer NanoZS), structure (TEM and CryoTEM), functional groups content (micro titration) and dispersion pH were analyzed in consecutive preparation stages. Results: BSA effectively prevented the aggregation of GO in precipitating concentrations of physiological bivalent cations. The final polydispersity indexes were reduced from 0.66–0.91 to 0.36–0.43. The GO-containing isotonic dispersions were stable with the following Z-ave results: GO1 421.1 nm, GO2 382.6 nm, GO3 440.2 nm and GO4 490.1 nm. The GO behavior was structure-dependent. Conclusion: BSA effectively stabilized four types of GO dispersions in an isotonic dispersion containing aggregating bivalent physiological cations.https://www.mdpi.com/1999-4923/15/10/2495graphene oxideaqueous dispersionstability of dispersionnanoparticle sizeion-induced aggregationbovine serum albumin |
| spellingShingle | Marcin Z. Krasoń Anna Paradowska Martyna Fronczek Mateusz Lejawa Natalia Kamieńska Michał Krejca Anna Kolanowska Sławomir Boncel Marek W. Radomski Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations Pharmaceutics graphene oxide aqueous dispersion stability of dispersion nanoparticle size ion-induced aggregation bovine serum albumin |
| title | Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations |
| title_full | Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations |
| title_fullStr | Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations |
| title_full_unstemmed | Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations |
| title_short | Stabilization of Graphene Oxide Dispersion in Plasma-like Isotonic Solution Containing Aggregating Concentrations of Bivalent Cations |
| title_sort | stabilization of graphene oxide dispersion in plasma like isotonic solution containing aggregating concentrations of bivalent cations |
| topic | graphene oxide aqueous dispersion stability of dispersion nanoparticle size ion-induced aggregation bovine serum albumin |
| url | https://www.mdpi.com/1999-4923/15/10/2495 |
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