Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum
Red microalga Porphyridium cruentum has great potential for converting CO2 into high-value bioactive compounds, such as B-phycoerythrin (B-PE) and extracellular polysaccharides or exopolysaccharides (EPS). This study aimed to establish the integration bioprocess of B-PE and EPS production from P. cr...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2021.836370/full |
| _version_ | 1819274165299970048 |
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| author | Hao-Chan Yin Ji-Kang Sui Tian-Li Han Tian-Li Han Tian-Zhong Liu Hui Wang |
| author_facet | Hao-Chan Yin Ji-Kang Sui Tian-Li Han Tian-Li Han Tian-Zhong Liu Hui Wang |
| author_sort | Hao-Chan Yin |
| collection | DOAJ |
| description | Red microalga Porphyridium cruentum has great potential for converting CO2 into high-value bioactive compounds, such as B-phycoerythrin (B-PE) and extracellular polysaccharides or exopolysaccharides (EPS). This study aimed to establish the integration bioprocess of B-PE and EPS production from P. cruentum. First, different kinds of growth medium and CO2 concentration were assessed indoor in terms of high biomass and B-PE and EPS contents. As follows, P. cruentum cells were outdoor scale-up cultured in 700 L pressurized tubular reactors for 9 days till the biomass reached 0.85 g/L and then separated from supernatants via centrifugation. Three different methods were adopted to extract phycobiliproteins, and the highest PE contents were extracted from cells by repeated freeze-thawing treatment along with the optimization of significant variables, and finally, 7.99 mg/L B-PE (16,500 Da) with a purity index of 0.82 was obtained. Moreover, analysis of physicochemical properties of EPS extracted from P. cruentum showed that the sulfate content was 14.85% and the uronic acid content was 9.36%. |
| first_indexed | 2024-12-23T23:04:05Z |
| format | Article |
| id | doaj.art-8e5a011f286a4b9ab12ecda00339c8cd |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-23T23:04:05Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-8e5a011f286a4b9ab12ecda00339c8cd2022-12-21T17:26:52ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-02-01810.3389/fmars.2021.836370836370Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentumHao-Chan Yin0Ji-Kang Sui1Tian-Li Han2Tian-Li Han3Tian-Zhong Liu4Hui Wang5Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaSchool of Chemical Engineering, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaKey Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaRed microalga Porphyridium cruentum has great potential for converting CO2 into high-value bioactive compounds, such as B-phycoerythrin (B-PE) and extracellular polysaccharides or exopolysaccharides (EPS). This study aimed to establish the integration bioprocess of B-PE and EPS production from P. cruentum. First, different kinds of growth medium and CO2 concentration were assessed indoor in terms of high biomass and B-PE and EPS contents. As follows, P. cruentum cells were outdoor scale-up cultured in 700 L pressurized tubular reactors for 9 days till the biomass reached 0.85 g/L and then separated from supernatants via centrifugation. Three different methods were adopted to extract phycobiliproteins, and the highest PE contents were extracted from cells by repeated freeze-thawing treatment along with the optimization of significant variables, and finally, 7.99 mg/L B-PE (16,500 Da) with a purity index of 0.82 was obtained. Moreover, analysis of physicochemical properties of EPS extracted from P. cruentum showed that the sulfate content was 14.85% and the uronic acid content was 9.36%.https://www.frontiersin.org/articles/10.3389/fmars.2021.836370/fullPorphyridiumbiomass productionphycoerythrinexopolysaccharideoutdoor scale-up culture |
| spellingShingle | Hao-Chan Yin Ji-Kang Sui Tian-Li Han Tian-Li Han Tian-Zhong Liu Hui Wang Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum Frontiers in Marine Science Porphyridium biomass production phycoerythrin exopolysaccharide outdoor scale-up culture |
| title | Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum |
| title_full | Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum |
| title_fullStr | Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum |
| title_full_unstemmed | Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum |
| title_short | Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum |
| title_sort | integration bioprocess of b phycoerythrin and exopolysaccharides production from photosynthetic microalga porphyridium cruentum |
| topic | Porphyridium biomass production phycoerythrin exopolysaccharide outdoor scale-up culture |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2021.836370/full |
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