Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography
pH-Zone-refining counter-current chromatography (pH-zone-refining CCC) was successfully applied for the large-scale separation of alkaloids from Corydalis bungeana. The crude extract was separated by a two-phase solvent system composed of petroleum ether–ethyl acetate–met...
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MDPI AG
2012-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/12/14968 |
| _version_ | 1828338965148598272 |
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| author | Bin Yang Luqi Huang Zhenjia Zheng Xikai Shu Xiao Wang Hongjing Dong |
| author_facet | Bin Yang Luqi Huang Zhenjia Zheng Xikai Shu Xiao Wang Hongjing Dong |
| author_sort | Bin Yang |
| collection | DOAJ |
| description | pH-Zone-refining counter-current chromatography (pH-zone-refining CCC) was successfully applied for the large-scale separation of alkaloids from Corydalis bungeana. The crude extract was separated by a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as a displacer. As a result, 285 mg of protopine, 86 mg of corynoloxine, 430 mg of coryno1ine, and 115 mg of acetylcorynoline were obtained from 3.0 g of crude extract in a one-step separation. The purities of these compounds were 99.1%, 98.3%, 99.0% and 98.5%, respectively, as determined by HPLC. The chemical structures of these isolated compounds were confirmed by ESI-MS, 1H-NMR and 13C-NMR. |
| first_indexed | 2024-04-13T22:36:22Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T22:36:22Z |
| publishDate | 2012-12-01 |
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| series | Molecules |
| spelling | doaj.art-8e5c58680b3644cfbac4d9180bcae1cc2022-12-22T02:26:45ZengMDPI AGMolecules1420-30492012-12-011712149681497410.3390/molecules171214968Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current ChromatographyBin YangLuqi HuangZhenjia ZhengXikai ShuXiao WangHongjing DongpH-Zone-refining counter-current chromatography (pH-zone-refining CCC) was successfully applied for the large-scale separation of alkaloids from Corydalis bungeana. The crude extract was separated by a two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v) where triethylamine (10 mM) was added to the upper organic stationary phase as a retainer and hydrochloric acid (5 mM) to the aqueous mobile phase as a displacer. As a result, 285 mg of protopine, 86 mg of corynoloxine, 430 mg of coryno1ine, and 115 mg of acetylcorynoline were obtained from 3.0 g of crude extract in a one-step separation. The purities of these compounds were 99.1%, 98.3%, 99.0% and 98.5%, respectively, as determined by HPLC. The chemical structures of these isolated compounds were confirmed by ESI-MS, 1H-NMR and 13C-NMR.http://www.mdpi.com/1420-3049/17/12/14968pH-zone-refining counter-current chromatographylarge-scale separationCorydalis bungeanaalkaloids |
| spellingShingle | Bin Yang Luqi Huang Zhenjia Zheng Xikai Shu Xiao Wang Hongjing Dong Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography Molecules pH-zone-refining counter-current chromatography large-scale separation Corydalis bungeana alkaloids |
| title | Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography |
| title_full | Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography |
| title_fullStr | Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography |
| title_full_unstemmed | Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography |
| title_short | Large-Scale Separation of Alkaloids from Corydalis bungeana Turcz. by pH-Zone-Refining Counter-Current Chromatography |
| title_sort | large scale separation of alkaloids from corydalis bungeana turcz by ph zone refining counter current chromatography |
| topic | pH-zone-refining counter-current chromatography large-scale separation Corydalis bungeana alkaloids |
| url | http://www.mdpi.com/1420-3049/17/12/14968 |
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