Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin
The transcription factor <i>bHLH</i> gene family plays fundamental roles in plant development and mitigating diverse biotic and abiotic stresses. However, the information of <i>bHLH</i> genes in pumpkin (<i>Cucurbita moschata</i>) is still unknown. In this current...
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MDPI AG
2023-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/6/648 |
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| author | Wenhong Ao Weirong Luo Wenchen Xu Xudong Wang Junjun Liu Yongdong Sun |
| author_facet | Wenhong Ao Weirong Luo Wenchen Xu Xudong Wang Junjun Liu Yongdong Sun |
| author_sort | Wenhong Ao |
| collection | DOAJ |
| description | The transcription factor <i>bHLH</i> gene family plays fundamental roles in plant development and mitigating diverse biotic and abiotic stresses. However, the information of <i>bHLH</i> genes in pumpkin (<i>Cucurbita moschata</i>) is still unknown. In this current study, 222 <i>CmobHLH</i> genes were identified and mapped onto different chromosomes through bioinformatics analysis in pumpkin. CmobHLH and AtbHLH proteins could be classified into 19 subfamilies according to the phylogenetic tree. CmobHLH proteins within the same subfamily had similar motif composition and gene structures. Gene ontology (GO), <i>cis</i>-regulatory elements (CREs) and protein–protein interaction analyses suggested the potential regulatory roles of <i>CmobHLH</i> genes during the plant development process and abiotic stresses response in pumpkin. Tissue expression patterns based on transcriptome data demonstrated that <i>CmobHLH</i> genes were involved in pumpkin development process, and they had unique functions in different tissues. The expression patterns of five selected <i>CmobHLH</i> genes after exposure to abiotic stresses showed that the <i>CmobHLH</i> genes played varied roles in the stress responses of pumpkin to NaCl, waterlogging, cold, ABA and drought. In brief, these findings offer important information for further functional research of <i>CmobHLH</i> genes and resistance breeding in pumpkin. |
| first_indexed | 2024-03-11T02:23:36Z |
| format | Article |
| id | doaj.art-8e5deddaf782417398259d3ea39a9b28 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T02:23:36Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-8e5deddaf782417398259d3ea39a9b282023-11-18T10:40:41ZengMDPI AGHorticulturae2311-75242023-05-019664810.3390/horticulturae9060648Molecular Characterization and Expression of <i>CmobHLH</i> Genes in PumpkinWenhong Ao0Weirong Luo1Wenchen Xu2Xudong Wang3Junjun Liu4Yongdong Sun5School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaThe transcription factor <i>bHLH</i> gene family plays fundamental roles in plant development and mitigating diverse biotic and abiotic stresses. However, the information of <i>bHLH</i> genes in pumpkin (<i>Cucurbita moschata</i>) is still unknown. In this current study, 222 <i>CmobHLH</i> genes were identified and mapped onto different chromosomes through bioinformatics analysis in pumpkin. CmobHLH and AtbHLH proteins could be classified into 19 subfamilies according to the phylogenetic tree. CmobHLH proteins within the same subfamily had similar motif composition and gene structures. Gene ontology (GO), <i>cis</i>-regulatory elements (CREs) and protein–protein interaction analyses suggested the potential regulatory roles of <i>CmobHLH</i> genes during the plant development process and abiotic stresses response in pumpkin. Tissue expression patterns based on transcriptome data demonstrated that <i>CmobHLH</i> genes were involved in pumpkin development process, and they had unique functions in different tissues. The expression patterns of five selected <i>CmobHLH</i> genes after exposure to abiotic stresses showed that the <i>CmobHLH</i> genes played varied roles in the stress responses of pumpkin to NaCl, waterlogging, cold, ABA and drought. In brief, these findings offer important information for further functional research of <i>CmobHLH</i> genes and resistance breeding in pumpkin.https://www.mdpi.com/2311-7524/9/6/648abiotic stresses<i>bHLH</i> genesbioinformatics analysis<i>Cucurbita moschata</i>expression analysis |
| spellingShingle | Wenhong Ao Weirong Luo Wenchen Xu Xudong Wang Junjun Liu Yongdong Sun Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin Horticulturae abiotic stresses <i>bHLH</i> genes bioinformatics analysis <i>Cucurbita moschata</i> expression analysis |
| title | Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin |
| title_full | Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin |
| title_fullStr | Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin |
| title_full_unstemmed | Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin |
| title_short | Molecular Characterization and Expression of <i>CmobHLH</i> Genes in Pumpkin |
| title_sort | molecular characterization and expression of i cmobhlh i genes in pumpkin |
| topic | abiotic stresses <i>bHLH</i> genes bioinformatics analysis <i>Cucurbita moschata</i> expression analysis |
| url | https://www.mdpi.com/2311-7524/9/6/648 |
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