Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)

Copper metal as a contact surface was studied during the germination of alfalfa seeds (Medicago sativa L.) inside a rotating drum on a laboratory scale and compared with a plastic surface of food-grade. A system of three rotating drums was used inside a thermo-regulated chamber to germinate seeds. A...

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Main Authors: José F. REYES, Johannes P.F. de BRUIJN, Guillermo F. TOLOSA, Pedro M. AQUEVEQUE, Christian L. CORREA
Format: Article
Language:English
Published: AcademicPres 2020-11-01
Series:Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
Subjects:
Online Access:https://journals.usamvcluj.ro/index.php/fst/article/view/13904
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author José F. REYES
Johannes P.F. de BRUIJN
Guillermo F. TOLOSA
Pedro M. AQUEVEQUE
Christian L. CORREA
author_facet José F. REYES
Johannes P.F. de BRUIJN
Guillermo F. TOLOSA
Pedro M. AQUEVEQUE
Christian L. CORREA
author_sort José F. REYES
collection DOAJ
description Copper metal as a contact surface was studied during the germination of alfalfa seeds (Medicago sativa L.) inside a rotating drum on a laboratory scale and compared with a plastic surface of food-grade. A system of three rotating drums was used inside a thermo-regulated chamber to germinate seeds. Alfalfa seeds were inoculated with 4.2 log cfu g-1 of Escherichia coli and after 84 h of germination sprouts were evaluated for E. coli, mesophilic aerobic bacteria, the content of copper and other minerals (potassium, calcium, magnesium, sodium, iron, manganese, and zinc), total mass, unit mass and length, and color. The contact of alfalfa sprouts with copper sheets allowed to reduce the E. coli load from 6.54 to <0.1 log cfu g-1. However, all sprouts exceeded in copper (> 10 ppm) according to Food Sanitary Regulations. Germinated mass and length decreased after copper treatments. No statistically significant differences were observed between treatments for the remaining quality parameters. Finally, it is concluded that copper was very efficient in reducing the microbial load of E. coli in alfalfa sprouts, complying with the regulations established by the Chilean Ministry of Health.
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spelling doaj.art-8e5e9a85a9e844c1ac5d270b8ed1c0fa2022-12-21T22:42:39ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002020-11-01772677610.15835/buasvmcn-fst:2020.004111345Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)José F. REYES0Johannes P.F. de BRUIJN1Guillermo F. TOLOSA2Pedro M. AQUEVEQUE3Christian L. CORREA4Faculty of Agricultural Engineering, Universidad de Concepción, Casilla 537, ChillánFaculty of Agricultural Engineering, Universidad de Concepción, Casilla 537, ChillánFaculty of Agricultural Engineering, Universidad de Concepción, Casilla 537, ChillánFaculty of Agricultural Engineering, Universidad de Concepción, Casilla 537, ChillánFaculty of Agricultural Engineering, Universidad de Concepción, Casilla 537, ChillánCopper metal as a contact surface was studied during the germination of alfalfa seeds (Medicago sativa L.) inside a rotating drum on a laboratory scale and compared with a plastic surface of food-grade. A system of three rotating drums was used inside a thermo-regulated chamber to germinate seeds. Alfalfa seeds were inoculated with 4.2 log cfu g-1 of Escherichia coli and after 84 h of germination sprouts were evaluated for E. coli, mesophilic aerobic bacteria, the content of copper and other minerals (potassium, calcium, magnesium, sodium, iron, manganese, and zinc), total mass, unit mass and length, and color. The contact of alfalfa sprouts with copper sheets allowed to reduce the E. coli load from 6.54 to <0.1 log cfu g-1. However, all sprouts exceeded in copper (> 10 ppm) according to Food Sanitary Regulations. Germinated mass and length decreased after copper treatments. No statistically significant differences were observed between treatments for the remaining quality parameters. Finally, it is concluded that copper was very efficient in reducing the microbial load of E. coli in alfalfa sprouts, complying with the regulations established by the Chilean Ministry of Health.https://journals.usamvcluj.ro/index.php/fst/article/view/13904sproutsalfalfaescherichia colicopperfood safety.
spellingShingle José F. REYES
Johannes P.F. de BRUIJN
Guillermo F. TOLOSA
Pedro M. AQUEVEQUE
Christian L. CORREA
Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
sprouts
alfalfa
escherichia coli
copper
food safety.
title Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
title_full Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
title_fullStr Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
title_full_unstemmed Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
title_short Antibacterial Effect of a Copper Surface in the Germination of Alfalfa Seeds (Medicago sativa L.)
title_sort antibacterial effect of a copper surface in the germination of alfalfa seeds medicago sativa l
topic sprouts
alfalfa
escherichia coli
copper
food safety.
url https://journals.usamvcluj.ro/index.php/fst/article/view/13904
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