Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology
Background Feline pancreas‐specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin) ester (DGGR) l...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-05-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.13924 |
| _version_ | 1811251419349516288 |
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| author | S. Oppliger M. Hilbe S. Hartnack E. Zini C.E. Reusch P.H. Kook |
| author_facet | S. Oppliger M. Hilbe S. Hartnack E. Zini C.E. Reusch P.H. Kook |
| author_sort | S. Oppliger |
| collection | DOAJ |
| description | Background Feline pancreas‐specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking. Objectives To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas. Animals Sixty client‐owned cats presented for necropsy. Methods Prospective study: Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined. Results The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4–55.9%] and 100% (95% CI, 31.0–100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7–50.7%) and 100% (95% CI, 31.0–100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1–81.7%) and 69.0% (95% CI, 52.8–81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2–85.6%) and 78.6% (95% CI, 62.8–89.2%). Conclusions and Clinical Importance Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited. |
| first_indexed | 2024-04-12T16:19:17Z |
| format | Article |
| id | doaj.art-8e5fcaedad024df08b073896879e2fc9 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-12T16:19:17Z |
| publishDate | 2016-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-8e5fcaedad024df08b073896879e2fc92022-12-22T03:25:37ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762016-05-0130376477010.1111/jvim.13924Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic HistologyS. Oppliger0M. Hilbe1S. Hartnack2E. Zini3C.E. Reusch4P.H. Kook5Clinic for Small Animal Internal Medicine Vetsuisse Faculty University of Zurich Zurich SwitzerlandInstitute for Veterinary Pathology Vetsuisse Faculty University of Zurich Zurich SwitzerlandSection of Epidemiology Vetsuisse Faculty University of Zurich Zurich SwitzerlandClinic for Small Animal Internal Medicine Vetsuisse Faculty University of Zurich Zurich SwitzerlandClinic for Small Animal Internal Medicine Vetsuisse Faculty University of Zurich Zurich SwitzerlandClinic for Small Animal Internal Medicine Vetsuisse Faculty University of Zurich Zurich SwitzerlandBackground Feline pancreas‐specific lipase (Spec fPL) is considered a useful test for the antemortem diagnosis of pancreatitis in cats. A recent study found good agreement between the results of the Spec fPL and catalytic 1,2‐o‐dilauryl‐rac‐glycero‐3‐glutaric acid‐(6′‐methylresorufin) ester (DGGR) lipase assay. Prospective studies evaluating their sensitivity and specificity are lacking. Objectives To compare the results of the Spec fPL and the DGGR assays with a standardized histologic assessment of the pancreas. Animals Sixty client‐owned cats presented for necropsy. Methods Prospective study: Spec fPL concentrations and serum DGGR lipase activity were measured from the same blood sample. The pancreas was removed within 3 hours after euthanasia; serial transverse sections were made every 0.5 cm throughout the entire pancreas and reviewed using a histologic grading scheme. Sensitivity and specificity for the Spec fPL and DGGR assay results were determined. Results The sensitivity and specificity for the Spec fPL assay (cutoff value ≥5.4 μg/L) was 42.1 [95% confidence interval (95% CI), 29.4–55.9%] and 100% (95% CI, 31.0–100.0%). The sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 36.8 (95% CI, 24.7–50.7%) and 100% (95% CI, 31.0–100.0%). When lymphocytic inflammation up to 10% of a section was considered normal, the sensitivity and specificity for Spec fPL assay (cutoff value ≥5.4 μg/L) was 61.1 (95% CI, 36.1–81.7%) and 69.0% (95% CI, 52.8–81.9%) and the sensitivity and specificity for the DGGR assay (cutoff value >26 U/L) was 66.7 (95% CI, 41.2–85.6%) and 78.6% (95% CI, 62.8–89.2%). Conclusions and Clinical Importance Both lipase assays performed similarly well, but their agreement with histologic pancreatic inflammation was limited.https://doi.org/10.1111/jvim.13924FelineFeline pancreas‐specific lipaseHistopathologyLipasePancreas |
| spellingShingle | S. Oppliger M. Hilbe S. Hartnack E. Zini C.E. Reusch P.H. Kook Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology Journal of Veterinary Internal Medicine Feline Feline pancreas‐specific lipase Histopathology Lipase Pancreas |
| title | Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology |
| title_full | Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology |
| title_fullStr | Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology |
| title_full_unstemmed | Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology |
| title_short | Comparison of Serum Spec fPL™ and 1,2‐o‐Dilauryl‐Rac‐Glycero‐3‐Glutaric Acid‐(6′‐Methylresorufin) Ester Assay in 60 Cats Using Standardized Assessment of Pancreatic Histology |
| title_sort | comparison of serum spec fpl™ and 1 2 o dilauryl rac glycero 3 glutaric acid 6 methylresorufin ester assay in 60 cats using standardized assessment of pancreatic histology |
| topic | Feline Feline pancreas‐specific lipase Histopathology Lipase Pancreas |
| url | https://doi.org/10.1111/jvim.13924 |
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