The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites
Background: Toxoplasmosis is one of the most common parasitic infections of humans and other mammals. This study was aimed to understand the mechanism of action of veterinary medicine-sulfachloropyrazine (SPZ, 99.97%) against Toxoplasma gondii. Methods: T. gondii tachyzoites were soaked in PBS (a...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-03-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/523 |
| _version_ | 1818885724309553152 |
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| author | Yb Zeng H Dong Hy Han Ll Jiang Qp Zhao Sh Zhu Wj Ma J Cheng B Huang |
| author_facet | Yb Zeng H Dong Hy Han Ll Jiang Qp Zhao Sh Zhu Wj Ma J Cheng B Huang |
| author_sort | Yb Zeng |
| collection | DOAJ |
| description | Background: Toxoplasmosis is one of the most common parasitic infections of humans and other mammals. This study was aimed to understand the mechanism of action of veterinary medicine-sulfachloropyrazine (SPZ, 99.97%) against Toxoplasma gondii.
Methods: T. gondii tachyzoites were soaked in PBS (as a control) or SPZ (250 mg/mL) for 2 h at 37 °C. After being processed, any ultrastructural changes of the tachyzoites that had occurred were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM).
Results: The tachyzoites from control groups with a uniform size had a smooth surface and intact cell or nuclear membranes. In addition, an oval-shaped nucleus, conoids and micronemes were also observed. By contrast, many parasites from the SPZ-treated groups were detrimentally affected by the treatment. Some appeared to be of the vacuolization in their cytoplasm, with the substantial re-duction in the number of dense granules and the blur of some organelles.
Conclusion: The morphology and ultrastructure of tachyzoites can be affected significantly by SPZ, which might kill the parasite by inhibiting its energy metabolism, inducing apoptosis and damaging its structure. The study provides an experimental basis for further study on the mechanism of SPZ against T. gondii. |
| first_indexed | 2024-12-19T16:09:59Z |
| format | Article |
| id | doaj.art-8e610ec959c1403fb0daeae482c6dbec |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T16:09:59Z |
| publishDate | 2013-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-8e610ec959c1403fb0daeae482c6dbec2022-12-21T20:14:46ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2013-03-0181The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii TachyzoitesYb Zeng0H Dong1Hy Han2Ll Jiang3Qp Zhao4Sh Zhu5Wj Ma6J Cheng7B Huang8Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Key Laboratory for Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academic of Agricultural Science, Shanghai 200241, China.Background: Toxoplasmosis is one of the most common parasitic infections of humans and other mammals. This study was aimed to understand the mechanism of action of veterinary medicine-sulfachloropyrazine (SPZ, 99.97%) against Toxoplasma gondii. Methods: T. gondii tachyzoites were soaked in PBS (as a control) or SPZ (250 mg/mL) for 2 h at 37 °C. After being processed, any ultrastructural changes of the tachyzoites that had occurred were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Results: The tachyzoites from control groups with a uniform size had a smooth surface and intact cell or nuclear membranes. In addition, an oval-shaped nucleus, conoids and micronemes were also observed. By contrast, many parasites from the SPZ-treated groups were detrimentally affected by the treatment. Some appeared to be of the vacuolization in their cytoplasm, with the substantial re-duction in the number of dense granules and the blur of some organelles. Conclusion: The morphology and ultrastructure of tachyzoites can be affected significantly by SPZ, which might kill the parasite by inhibiting its energy metabolism, inducing apoptosis and damaging its structure. The study provides an experimental basis for further study on the mechanism of SPZ against T. gondii.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/523SulfachloropyrazineTachyzoitesToxoplasma gondiiUltrastructure |
| spellingShingle | Yb Zeng H Dong Hy Han Ll Jiang Qp Zhao Sh Zhu Wj Ma J Cheng B Huang The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites Iranian Journal of Parasitology Sulfachloropyrazine Tachyzoites Toxoplasma gondii Ultrastructure |
| title | The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites |
| title_full | The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites |
| title_fullStr | The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites |
| title_full_unstemmed | The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites |
| title_short | The Ultrastructural Effects of Sulfachloropyrazine on Toxoplasma gondii Tachyzoites |
| title_sort | ultrastructural effects of sulfachloropyrazine on toxoplasma gondii tachyzoites |
| topic | Sulfachloropyrazine Tachyzoites Toxoplasma gondii Ultrastructure |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/523 |
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