Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia
We analyzed the blood from 400 one-humped camels, <i>Camelus dromedarius</i> (<i>C. dromedarius</i>)<i>,</i> in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite <i>Trypanosoma</i> spp. Polymerase chain reaction (P...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/11/4/1149 |
| _version_ | 1827694853292556288 |
|---|---|
| author | Dina M. Metwally Isra M. Al-Turaiki Najwa Altwaijry Samia Q. Alghamdi Abdullah D. Alanazi |
| author_facet | Dina M. Metwally Isra M. Al-Turaiki Najwa Altwaijry Samia Q. Alghamdi Abdullah D. Alanazi |
| author_sort | Dina M. Metwally |
| collection | DOAJ |
| description | We analyzed the blood from 400 one-humped camels, <i>Camelus dromedarius</i> (<i>C. dromedarius</i>)<i>,</i> in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite <i>Trypanosoma</i> spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of <i>Trypanosoma</i> spp. in the camels. <i>Trypanosoma evansi</i> (<i>T. evansi</i>) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated <i>T. evansi</i> was closely related to the <i>T. evansi</i> that was detected in <i>C. dromedarius</i> in Egypt and the <i>T. evansi</i> strain <i>B15.1 18S</i> ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of <i>T. evansi</i> from beef cattle in Thailand and to <i>T. brucei B8/18</i> 18S ribosomal RNA from pigs in Nigeria. |
| first_indexed | 2024-03-10T12:14:35Z |
| format | Article |
| id | doaj.art-8e61fc974376407483b8bf04b8c8b70e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:14:35Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-8e61fc974376407483b8bf04b8c8b70e2023-11-21T15:58:51ZengMDPI AGAnimals2076-26152021-04-01114114910.3390/ani11041149Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi ArabiaDina M. Metwally0Isra M. Al-Turaiki1Najwa Altwaijry2Samia Q. Alghamdi3Abdullah D. Alanazi4Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Information Technology, College of Computer and Information Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Computer Science, College of Computer and Information Sciences, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Biology, Faculty of Science, Al-Baha University, Al-Baha 1988, Saudi ArabiaDepartment of Biological Science, Faculty of Science and Humanities, Shaqra University, Ad-Dawadimi 11911, Saudi ArabiaWe analyzed the blood from 400 one-humped camels, <i>Camelus dromedarius</i> (<i>C. dromedarius</i>)<i>,</i> in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite <i>Trypanosoma</i> spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of <i>Trypanosoma</i> spp. in the camels. <i>Trypanosoma evansi</i> (<i>T. evansi</i>) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated <i>T. evansi</i> was closely related to the <i>T. evansi</i> that was detected in <i>C. dromedarius</i> in Egypt and the <i>T. evansi</i> strain <i>B15.1 18S</i> ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of <i>T. evansi</i> from beef cattle in Thailand and to <i>T. brucei B8/18</i> 18S ribosomal RNA from pigs in Nigeria.https://www.mdpi.com/2076-2615/11/4/1149<i>Trypanosoma</i> spp.camelITS-1Saudi ArabiaSurrapolymerase chain reaction (PCR) |
| spellingShingle | Dina M. Metwally Isra M. Al-Turaiki Najwa Altwaijry Samia Q. Alghamdi Abdullah D. Alanazi Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia Animals <i>Trypanosoma</i> spp. camel ITS-1 Saudi Arabia Surra polymerase chain reaction (PCR) |
| title | Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia |
| title_full | Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia |
| title_fullStr | Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia |
| title_full_unstemmed | Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia |
| title_short | Molecular Identification of <i>Trypanosoma evansi</i> Isolated from Arabian Camels (<i>Camelus dromedarius</i>) in Riyadh and Al-Qassim, Saudi Arabia |
| title_sort | molecular identification of i trypanosoma evansi i isolated from arabian camels i camelus dromedarius i in riyadh and al qassim saudi arabia |
| topic | <i>Trypanosoma</i> spp. camel ITS-1 Saudi Arabia Surra polymerase chain reaction (PCR) |
| url | https://www.mdpi.com/2076-2615/11/4/1149 |
| work_keys_str_mv | AT dinammetwally molecularidentificationofitrypanosomaevansiiisolatedfromarabiancamelsicamelusdromedariusiinriyadhandalqassimsaudiarabia AT isramalturaiki molecularidentificationofitrypanosomaevansiiisolatedfromarabiancamelsicamelusdromedariusiinriyadhandalqassimsaudiarabia AT najwaaltwaijry molecularidentificationofitrypanosomaevansiiisolatedfromarabiancamelsicamelusdromedariusiinriyadhandalqassimsaudiarabia AT samiaqalghamdi molecularidentificationofitrypanosomaevansiiisolatedfromarabiancamelsicamelusdromedariusiinriyadhandalqassimsaudiarabia AT abdullahdalanazi molecularidentificationofitrypanosomaevansiiisolatedfromarabiancamelsicamelusdromedariusiinriyadhandalqassimsaudiarabia |