Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products
Pseudomonas species is one of the psychotropic bacteria that can survive in low-tempered milk and dairy products besides producing heat-resistant spoilage enzymes. In this study, one hundred and fifty samples of milk and some dairy products were analyzed. The overall prevalence of Pseudomonas spp....
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2022-09-01
|
| Series: | Journal of Advanced Veterinary Research |
| Subjects: | |
| Online Access: | https://advetresearch.com/index.php/AVR/article/view/1037 |
| _version_ | 1797656992172474368 |
|---|---|
| author | Heba Gamal Mohamed El-Diasty Amany Dapgh Mohammed El-Sherbini Amira El-Baz Adel Abdelkhalek |
| author_facet | Heba Gamal Mohamed El-Diasty Amany Dapgh Mohammed El-Sherbini Amira El-Baz Adel Abdelkhalek |
| author_sort | Heba Gamal |
| collection | DOAJ |
| description |
Pseudomonas species is one of the psychotropic bacteria that can survive in low-tempered milk and dairy products besides producing heat-resistant spoilage enzymes. In this study, one hundred and fifty samples of milk and some dairy products were analyzed. The overall prevalence of Pseudomonas spp. was 44.66% (0% pasteurized milk, 16% butter, 20% pasteurized cream, 48.5% Talaga cheese, 50% bulk milk tank, 66.6% raw market milk, and 70% in raw cream). From 67 positive samples, eighty-three isolates were confirmed biochemically as Pseudomonas spp. The most prominent species were P. aeruginosa, then P. fluorescence, P. Fragi, P. psychrophile, P. proteolytica, P. alcaligens, P. lundensis, and P. brenneri by a percent of 38.5%, 37.5%, 10.8%, 6%, 2.4%, 2.4%, 1.2%, and 1.2%, respectively. Fourteen antibiotic discs were selected to measure the antimicrobial susceptibility of 59 isolates of Pseudomonas spp. The higher antimicrobial resistance was against Ampicillin (100%) followed by Colistin (98%), while the antibiotic sensitivity was higher against Imipenem (96.6%) then Meropenem (91.5%). The average MAR index of isolated Pseudomonas spp. was 0.462. Ten isolates of antimicrobial resistance serotypes of P. aeruginosa were O11: E, O8: C, O5: B, O4: F, and O2: B. Molecular identification of P. aeruginosa, P. fluorescence, and P. fragi was carried out using polymerase chain reaction (PCR) to determine their virulence genes (LasB, ExoS, pilB for P. aeruginosa, aprX for P. fluorescence and carA gene for P. fragi). High levels of antimicrobial-resistant (AMR) Pseudomonas spp. threaten public health and cause global concern. The economic and public health impacts were discussed.
|
| first_indexed | 2024-03-11T17:38:55Z |
| format | Article |
| id | doaj.art-8e62cb0d7f2b4b4dabfd5b49745f3f2b |
| institution | Directory Open Access Journal |
| issn | 2090-6269 2090-6277 |
| language | English |
| last_indexed | 2024-03-11T17:38:55Z |
| publishDate | 2022-09-01 |
| publisher | Assiut University |
| record_format | Article |
| series | Journal of Advanced Veterinary Research |
| spelling | doaj.art-8e62cb0d7f2b4b4dabfd5b49745f3f2b2023-10-18T14:26:04ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772022-09-011241001Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products Heba Gamal0Mohamed El-Diasty1Amany Dapgh2Mohammed El-Sherbini3Amira El-Baz4Adel Abdelkhalek5Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.Agricultural Research Center (ARC), Animal Health Research Institute- Mansoura provincial Lab (AHRI-Mansoura) P.O. Box 264-Giza, Cairo 12618, Egypt.Agricultural Research Center (ARC), Animal Health Research Institute- Mansoura provincial Lab (AHRI-Mansoura) P.O. Box 264-Giza, Cairo 12618, Egypt.Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.Department of Food Hygiene and Control, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.School of Veterinary Medicine, Badr University in Cairo (BUC) P.O. Box 11829. Pseudomonas species is one of the psychotropic bacteria that can survive in low-tempered milk and dairy products besides producing heat-resistant spoilage enzymes. In this study, one hundred and fifty samples of milk and some dairy products were analyzed. The overall prevalence of Pseudomonas spp. was 44.66% (0% pasteurized milk, 16% butter, 20% pasteurized cream, 48.5% Talaga cheese, 50% bulk milk tank, 66.6% raw market milk, and 70% in raw cream). From 67 positive samples, eighty-three isolates were confirmed biochemically as Pseudomonas spp. The most prominent species were P. aeruginosa, then P. fluorescence, P. Fragi, P. psychrophile, P. proteolytica, P. alcaligens, P. lundensis, and P. brenneri by a percent of 38.5%, 37.5%, 10.8%, 6%, 2.4%, 2.4%, 1.2%, and 1.2%, respectively. Fourteen antibiotic discs were selected to measure the antimicrobial susceptibility of 59 isolates of Pseudomonas spp. The higher antimicrobial resistance was against Ampicillin (100%) followed by Colistin (98%), while the antibiotic sensitivity was higher against Imipenem (96.6%) then Meropenem (91.5%). The average MAR index of isolated Pseudomonas spp. was 0.462. Ten isolates of antimicrobial resistance serotypes of P. aeruginosa were O11: E, O8: C, O5: B, O4: F, and O2: B. Molecular identification of P. aeruginosa, P. fluorescence, and P. fragi was carried out using polymerase chain reaction (PCR) to determine their virulence genes (LasB, ExoS, pilB for P. aeruginosa, aprX for P. fluorescence and carA gene for P. fragi). High levels of antimicrobial-resistant (AMR) Pseudomonas spp. threaten public health and cause global concern. The economic and public health impacts were discussed. https://advetresearch.com/index.php/AVR/article/view/1037AMR, Dairy products, milk, PCR, Pseudomonas spp |
| spellingShingle | Heba Gamal Mohamed El-Diasty Amany Dapgh Mohammed El-Sherbini Amira El-Baz Adel Abdelkhalek Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products Journal of Advanced Veterinary Research AMR, Dairy products, milk, PCR, Pseudomonas spp |
| title | Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products |
| title_full | Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products |
| title_fullStr | Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products |
| title_full_unstemmed | Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products |
| title_short | Virulence Genes of Multi-drug Resistance Pseudomonas species Isolated from Milk and Some Dairy Products |
| title_sort | virulence genes of multi drug resistance pseudomonas species isolated from milk and some dairy products |
| topic | AMR, Dairy products, milk, PCR, Pseudomonas spp |
| url | https://advetresearch.com/index.php/AVR/article/view/1037 |
| work_keys_str_mv | AT hebagamal virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts AT mohamedeldiasty virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts AT amanydapgh virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts AT mohammedelsherbini virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts AT amiraelbaz virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts AT adelabdelkhalek virulencegenesofmultidrugresistancepseudomonasspeciesisolatedfrommilkandsomedairyproducts |