Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome
The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been th...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2011-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019 |
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| author | Santosh K. Tiwari Vishwas Sharma Varun Kumar Sharma Manoj Gopi R Saikant Amrita Nandan Avinash Bardia Sivaram Gunisetty Prasanth Katikala Md. Aejaz Habeeb Aleem A. Khan C.M. Habibullah |
| author_facet | Santosh K. Tiwari Vishwas Sharma Varun Kumar Sharma Manoj Gopi R Saikant Amrita Nandan Avinash Bardia Sivaram Gunisetty Prasanth Katikala Md. Aejaz Habeeb Aleem A. Khan C.M. Habibullah |
| author_sort | Santosh K. Tiwari |
| collection | DOAJ |
| description | The population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine phosphorylation motifs (TPMs), in 32 H. pylori strains isolated from subjects with several forms of gastric diseases, was also explored. High resolution sequence analysis of the above described genes was performed. The nucleotide sequences obtained were translated into amino acids using MEGA (version 4.0) software for EPIYA. An MJ-Network was constructed for obtaining TPM haplotypes by using NETWORK (version 4.5) software. The findings of the study suggest that Indian H. pylori strains share a common ancestry with Europeans. No specific association of haplotypes with the outcome of disease was revealed through additional network analysis of TPMs. |
| first_indexed | 2024-04-12T07:05:01Z |
| format | Article |
| id | doaj.art-8e6338f957074171b7df6108acb37984 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-12T07:05:01Z |
| publishDate | 2011-01-01 |
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| series | Genetics and Molecular Biology |
| spelling | doaj.art-8e6338f957074171b7df6108acb379842022-12-22T03:42:50ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-01342280285Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcomeSantosh K. TiwariVishwas SharmaVarun Kumar SharmaManoj GopiR SaikantAmrita NandanAvinash BardiaSivaram GunisettyPrasanth KatikalaMd. Aejaz HabeebAleem A. KhanC.M. HabibullahThe population of India harbors one of the world's most highly diverse gene pools, owing to the influx of successive waves of immigrants over regular periods in time. Several phylogenetic studies involving mitochondrial DNA and Y chromosomal variation have demonstrated Europeans to have been the first settlers in India. Nevertheless, certain controversy exists, due to the support given to the thesis that colonization was by the Austro-Asiatic group, prior to the Europeans. Thus, the aim was to investigate pre-historic colonization of India by anatomically modern humans, using conserved stretches of five amino acid (EPIYA) sequences in the cagA gene of Helicobacter pylori. Simultaneously, the existence of a pathogenic relationship of tyrosine phosphorylation motifs (TPMs), in 32 H. pylori strains isolated from subjects with several forms of gastric diseases, was also explored. High resolution sequence analysis of the above described genes was performed. The nucleotide sequences obtained were translated into amino acids using MEGA (version 4.0) software for EPIYA. An MJ-Network was constructed for obtaining TPM haplotypes by using NETWORK (version 4.5) software. The findings of the study suggest that Indian H. pylori strains share a common ancestry with Europeans. No specific association of haplotypes with the outcome of disease was revealed through additional network analysis of TPMs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019Helicobacter pyloriEPIYA motifstyrosine phosphorylation motifshaplotypesanatomically modern humans |
| spellingShingle | Santosh K. Tiwari Vishwas Sharma Varun Kumar Sharma Manoj Gopi R Saikant Amrita Nandan Avinash Bardia Sivaram Gunisetty Prasanth Katikala Md. Aejaz Habeeb Aleem A. Khan C.M. Habibullah Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome Genetics and Molecular Biology Helicobacter pylori EPIYA motifs tyrosine phosphorylation motifs haplotypes anatomically modern humans |
| title | Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| title_full | Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| title_fullStr | Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| title_full_unstemmed | Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| title_short | Phylogenetic analysis, based on EPIYA repeats in the cagA gene of Indian Helicobacter pylori, and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| title_sort | phylogenetic analysis based on epiya repeats in the caga gene of indian helicobacter pylori and the implications of sequence variation in tyrosine phosphorylation motifs on determining the clinical outcome |
| topic | Helicobacter pylori EPIYA motifs tyrosine phosphorylation motifs haplotypes anatomically modern humans |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200019 |
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