Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells
Background: Increased dietary consumption of the n-3 polyunsaturated fatty acids (PUFA) eicosapetaenoic acid (20: 5n-3; EPA) and docosahexaenoic acid (22: 6n-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The rela...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2009-08-01
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| Series: | مجله دانشکده پزشکی اصفهان |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/275 |
| _version_ | 1797722214857965568 |
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| author | Yousef Shabani Reza Rastmanesh Saeed Kaviani Ahmad Reza Jamshidi |
| author_facet | Yousef Shabani Reza Rastmanesh Saeed Kaviani Ahmad Reza Jamshidi |
| author_sort | Yousef Shabani |
| collection | DOAJ |
| description | Background:
Increased dietary consumption of the n-3 polyunsaturated fatty acids (PUFA) eicosapetaenoic acid (20: 5n-3; EPA) and docosahexaenoic acid (22: 6n-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The relationship between peripheral blood mononuclear cell (PBMC) function, n-3 PUFA intake and antioxidant co-supplementation is poorly defined in rheumatoid arthritis (RA) patients. This study was designed to evaluate tumor necrosis factor-α (TNF-α) and interleukin (IL) 6 production by PBMC in RA.
Methods:
Fifty-five patients (50 female, 5 male; mean age = 47 ± 11 years) were investigated in three groups in a randomized double-blind clinical trial. Patients were randomly assigned to one of these three groups: group 1 (G1) was received placebo (MCT oil, 2 g/d plus vitamin E placebo), the second group (G2) was received n-3 fatty acids (1.2 g/d; EPA/DHA plus vitamin E placebo), and the third group (G3) was received n-3 fatty acids (1.2 g/d; EPA/DHA) plus 100 IU/d vitamin E. Medication dose was intact during the study. Data were compared between groups using analysis of variance (ANOVA).
Findings:
Fish oil n-3 fatty acids supplementation of diet decreased production of TNF-α (P = 0.007) by stimulated PBMC in G2 and G3 in relation to G1. Also G3 in relation to G2 and G1 showed significant decrease with respect to malonaldehyde (MDA) (P = 0.005).
Conclusions:
Consumption of omega-3 fatty acids with vitamin E not only decreased production of inflammatory parameters but also decreased lipid per-oxidation in RA patients.
Key words: Rheumatoid arthritis, PBMC, N-3 fatty acids, Vitamin E. |
| first_indexed | 2024-03-12T09:44:17Z |
| format | Article |
| id | doaj.art-8e65ecc00eca412b82c2f1c1b2f46228 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T09:44:17Z |
| publishDate | 2009-08-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-8e65ecc00eca412b82c2f1c1b2f462282023-09-02T13:00:56ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2009-08-012797352364267Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear CellsYousef Shabani0Reza Rastmanesh1Saeed Kaviani2Ahmad Reza Jamshidi3Master of Science, Department of Nutrition and Food Technology, School of Health, Shaheed Beheshti University of Medical Sciences, TehranAssistant Professor, Department of Human Nutrition, Shaheed Beheshti University of Medical Sciences, TehranAssistant Professor, Department of Hematology, Tehran University of Tarbiat Modarres, TehranAssociate Professor, Tehran Rheumatology Research Center, Tehran University of Medical Sciences, TehranBackground: Increased dietary consumption of the n-3 polyunsaturated fatty acids (PUFA) eicosapetaenoic acid (20: 5n-3; EPA) and docosahexaenoic acid (22: 6n-6; DHA) is associated with their incorporation into circulating phospholipid and increased production of lipid peroxide metabolites. The relationship between peripheral blood mononuclear cell (PBMC) function, n-3 PUFA intake and antioxidant co-supplementation is poorly defined in rheumatoid arthritis (RA) patients. This study was designed to evaluate tumor necrosis factor-α (TNF-α) and interleukin (IL) 6 production by PBMC in RA. Methods: Fifty-five patients (50 female, 5 male; mean age = 47 ± 11 years) were investigated in three groups in a randomized double-blind clinical trial. Patients were randomly assigned to one of these three groups: group 1 (G1) was received placebo (MCT oil, 2 g/d plus vitamin E placebo), the second group (G2) was received n-3 fatty acids (1.2 g/d; EPA/DHA plus vitamin E placebo), and the third group (G3) was received n-3 fatty acids (1.2 g/d; EPA/DHA) plus 100 IU/d vitamin E. Medication dose was intact during the study. Data were compared between groups using analysis of variance (ANOVA). Findings: Fish oil n-3 fatty acids supplementation of diet decreased production of TNF-α (P = 0.007) by stimulated PBMC in G2 and G3 in relation to G1. Also G3 in relation to G2 and G1 showed significant decrease with respect to malonaldehyde (MDA) (P = 0.005). Conclusions: Consumption of omega-3 fatty acids with vitamin E not only decreased production of inflammatory parameters but also decreased lipid per-oxidation in RA patients. Key words: Rheumatoid arthritis, PBMC, N-3 fatty acids, Vitamin E.http://jims.mui.ac.ir/index.php/jims/article/view/275 |
| spellingShingle | Yousef Shabani Reza Rastmanesh Saeed Kaviani Ahmad Reza Jamshidi Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells مجله دانشکده پزشکی اصفهان |
| title | Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells |
| title_full | Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells |
| title_fullStr | Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells |
| title_full_unstemmed | Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells |
| title_short | Effect of N-3 Fatty Acids Supplementation on Production of Inflammatory Cytokines by Peripheral Mononuclear Cells |
| title_sort | effect of n 3 fatty acids supplementation on production of inflammatory cytokines by peripheral mononuclear cells |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/275 |
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