Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus
Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4) is a devastating phytopathogen responsible for significant losses in banana production worldwide. Trichoderma and other biocontrol agents (BCAs) have been used as suitable disease control methods for banana Fusarium wilt. In this study, the...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-11-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1301062/full |
| _version_ | 1797634977015267328 |
|---|---|
| author | Mei Luo Mei Luo Yue Chen Qiurong Huang Zhenxin Huang Handa Song Handa Song Zhangyong Dong Zhangyong Dong |
| author_facet | Mei Luo Mei Luo Yue Chen Qiurong Huang Zhenxin Huang Handa Song Handa Song Zhangyong Dong Zhangyong Dong |
| author_sort | Mei Luo |
| collection | DOAJ |
| description | Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4) is a devastating phytopathogen responsible for significant losses in banana production worldwide. Trichoderma and other biocontrol agents (BCAs) have been used as suitable disease control methods for banana Fusarium wilt. In this study, the endophytic T. koningiopsis Tk905 strain was isolated from the roots of dendrobe plants and identified utilizing morphological and molecular analyses. Antifungal activity tests revealed that Tk905 effectively inhibited mycelial growth with inhibition rates ranging from 26.52 to 75.34%. Additionally, Tk905 covered the pathogen mycelia, and spores were observed on or around the pathogen hyphae. The average root and shoot fresh weights and plant height, of Tk905-inoculated plants were significantly higher than those of the untreated plants. Furthermore, Tk905 treatment significantly increased the activity of antioxidant enzymes, such as catalase (CAT), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD), suggesting that Tk905 may enhance plant defence systems by activating their antioxidant mechanisms. Most importantly, Tk905-treated plants inoculated by three methods exhibited significantly lower disease incidence and severity than untreated plants. The protective effects of Tk905 against FocTR4 infection were not only observed in the early stages of infection but persisted throughout the experiment, suggesting that T. koningiopsis Tk905 can provide long-lasting protection against Fusarium wilt. |
| first_indexed | 2024-03-11T12:14:54Z |
| format | Article |
| id | doaj.art-8e660f435b384ae09b382cf7a3d0edc3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T12:14:54Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8e660f435b384ae09b382cf7a3d0edc32023-11-07T10:07:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.13010621301062Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungusMei Luo0Mei Luo1Yue Chen2Qiurong Huang3Zhenxin Huang4Handa Song5Handa Song6Zhangyong Dong7Zhangyong Dong8Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaKey laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaKey laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaKey laboratory of Fruit and Vegetable Green Prevention and Control in South-China, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaFusarium oxysporum f. sp. cubense tropical race 4 (FocTR4) is a devastating phytopathogen responsible for significant losses in banana production worldwide. Trichoderma and other biocontrol agents (BCAs) have been used as suitable disease control methods for banana Fusarium wilt. In this study, the endophytic T. koningiopsis Tk905 strain was isolated from the roots of dendrobe plants and identified utilizing morphological and molecular analyses. Antifungal activity tests revealed that Tk905 effectively inhibited mycelial growth with inhibition rates ranging from 26.52 to 75.34%. Additionally, Tk905 covered the pathogen mycelia, and spores were observed on or around the pathogen hyphae. The average root and shoot fresh weights and plant height, of Tk905-inoculated plants were significantly higher than those of the untreated plants. Furthermore, Tk905 treatment significantly increased the activity of antioxidant enzymes, such as catalase (CAT), phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD), suggesting that Tk905 may enhance plant defence systems by activating their antioxidant mechanisms. Most importantly, Tk905-treated plants inoculated by three methods exhibited significantly lower disease incidence and severity than untreated plants. The protective effects of Tk905 against FocTR4 infection were not only observed in the early stages of infection but persisted throughout the experiment, suggesting that T. koningiopsis Tk905 can provide long-lasting protection against Fusarium wilt.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1301062/fullTrichoderma koningiopsissoil-borne diseaseinduced resistanceplant-growth promotingbiocontrol |
| spellingShingle | Mei Luo Mei Luo Yue Chen Qiurong Huang Zhenxin Huang Handa Song Handa Song Zhangyong Dong Zhangyong Dong Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus Frontiers in Microbiology Trichoderma koningiopsis soil-borne disease induced resistance plant-growth promoting biocontrol |
| title | Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus |
| title_full | Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus |
| title_fullStr | Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus |
| title_full_unstemmed | Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus |
| title_short | Trichoderma koningiopsis Tk905: an efficient biocontrol, induced resistance agent against banana Fusarium wilt disease and a potential plant-growth-promoting fungus |
| title_sort | trichoderma koningiopsis tk905 an efficient biocontrol induced resistance agent against banana fusarium wilt disease and a potential plant growth promoting fungus |
| topic | Trichoderma koningiopsis soil-borne disease induced resistance plant-growth promoting biocontrol |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1301062/full |
| work_keys_str_mv | AT meiluo trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT meiluo trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT yuechen trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT qiuronghuang trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT zhenxinhuang trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT handasong trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT handasong trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT zhangyongdong trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus AT zhangyongdong trichodermakoningiopsistk905anefficientbiocontrolinducedresistanceagentagainstbananafusariumwiltdiseaseandapotentialplantgrowthpromotingfungus |