Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways
The role of G-protein and mitogen-activated protein kinase (MAPK) in filamentous fungi has been studied for over a decade, but downstream elements are less known. Here, we used microarray and Northern blot analysis to examine the involvement of these signalling pathways in controlling the knownCochl...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-06-01
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| Series: | Plant Protection Science |
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| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201502-0001_cochliobolus-heterostrophus-t-toxin-gene-expression-modulation-via-g-protein-and-mapk-pathways.php |
| _version_ | 1797898498550530048 |
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| author | Ofir DEGANI |
| author_facet | Ofir DEGANI |
| author_sort | Ofir DEGANI |
| collection | DOAJ |
| description | The role of G-protein and mitogen-activated protein kinase (MAPK) in filamentous fungi has been studied for over a decade, but downstream elements are less known. Here, we used microarray and Northern blot analysis to examine the involvement of these signalling pathways in controlling the knownCochliobolus heterostrophus T-toxin biosynthetic gene, DEC1, whose control is important in epidemic prevention. Comparison of the expression profile in wild-type strains and in G-protein and MAPK signalling deficient mutants revealed a unique, environmental-dependent control mechanism for the DEC1 gene. The results suggest, for the first time, a possible role in pathogenicity for the G-protein α2 subunit in this organism, and hint to a common role of G-protein α1 and β1 subunits and MAPK in maintaining accurate levels of this toxin during pathogenesis. |
| first_indexed | 2024-04-10T08:15:19Z |
| format | Article |
| id | doaj.art-8e6660605f3249a9a55919e5dda0cb26 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:15:19Z |
| publishDate | 2015-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-8e6660605f3249a9a55919e5dda0cb262023-02-23T03:44:14ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412015-06-01512536010.17221/67/2014-PPSpps-201502-0001Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathwaysOfir DEGANI0Tel-Hai College, Upper Galilee, IsraelThe role of G-protein and mitogen-activated protein kinase (MAPK) in filamentous fungi has been studied for over a decade, but downstream elements are less known. Here, we used microarray and Northern blot analysis to examine the involvement of these signalling pathways in controlling the knownCochliobolus heterostrophus T-toxin biosynthetic gene, DEC1, whose control is important in epidemic prevention. Comparison of the expression profile in wild-type strains and in G-protein and MAPK signalling deficient mutants revealed a unique, environmental-dependent control mechanism for the DEC1 gene. The results suggest, for the first time, a possible role in pathogenicity for the G-protein α2 subunit in this organism, and hint to a common role of G-protein α1 and β1 subunits and MAPK in maintaining accurate levels of this toxin during pathogenesis.https://pps.agriculturejournals.cz/artkey/pps-201502-0001_cochliobolus-heterostrophus-t-toxin-gene-expression-modulation-via-g-protein-and-mapk-pathways.phpcochliobolus heterostrophusdec1 genefungal rnamaizesignal transductionsouthern corn leaf blight |
| spellingShingle | Ofir DEGANI Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways Plant Protection Science cochliobolus heterostrophus dec1 gene fungal rna maize signal transduction southern corn leaf blight |
| title | Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways |
| title_full | Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways |
| title_fullStr | Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways |
| title_full_unstemmed | Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways |
| title_short | Cochliobolus heterostrophus T-toxin gene expression modulation via G protein and MAPK pathways |
| title_sort | cochliobolus heterostrophus t toxin gene expression modulation via g protein and mapk pathways |
| topic | cochliobolus heterostrophus dec1 gene fungal rna maize signal transduction southern corn leaf blight |
| url | https://pps.agriculturejournals.cz/artkey/pps-201502-0001_cochliobolus-heterostrophus-t-toxin-gene-expression-modulation-via-g-protein-and-mapk-pathways.php |
| work_keys_str_mv | AT ofirdegani cochliobolusheterostrophusttoxingeneexpressionmodulationviagproteinandmapkpathways |