Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil
To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA....
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2012-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600015&lng=en&tlng=en |
| _version_ | 1797712548075667456 |
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| author | Teresinha Teixeira de Sousa Menira Souza Fabíola Souza Fiaccadori Ana Maria Tavares Borges Paulo Sucasas da Costa Divina das Dôres de Paula Cardoso |
| author_facet | Teresinha Teixeira de Sousa Menira Souza Fabíola Souza Fiaccadori Ana Maria Tavares Borges Paulo Sucasas da Costa Divina das Dôres de Paula Cardoso |
| author_sort | Teresinha Teixeira de Sousa |
| collection | DOAJ |
| description | To determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis. |
| first_indexed | 2024-03-12T07:23:23Z |
| format | Article |
| id | doaj.art-8e67597f471e4f219a37b9fa1d910a1b |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:23:23Z |
| publishDate | 2012-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-8e67597f471e4f219a37b9fa1d910a1b2023-09-02T22:14:59ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602012-09-01107680080410.1590/S0074-02762012000600015S0074-02762012000600015Human bocavirus 1 and 3 infection in children with acute gastroenteritis in BrazilTeresinha Teixeira de Sousa0Menira Souza1Fabíola Souza Fiaccadori2Ana Maria Tavares Borges3Paulo Sucasas da Costa4Divina das Dôres de Paula Cardoso5Universidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásUniversidade Federal de GoiásTo determine the positivity rate of human bocavirus (HBoV) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the Central-West Region of Brazil, 762 faecal samples were tested using polymerase chain reaction (PCR) for the detection of HBoV DNA. Primers for a segment of the non-structural viral protein 1 (NS1) gene of HBoV-1 and HBoV-3 were used. Twelve HBoV-positive samples were further characterised via genomic sequencing and phylogenetic analysis. Of the samples tested, 5.8% (n = 44) were positive for HBoV-1 or HBoV-3 and co-infection was observed in 14 (31.8%) of the 44 HBoV-positive samples. Nine of the 14 samples were also positive for Rotavirus A and five were positive for Aichi virus. The genomic sequencing of the NS1 partial sequence of 12 HBoV-samples showed that 11 samples were characterised as HBoV-1 and that one was characterised as HBoV-3. The phylogenetic analysis showed that the HBoV-1 samples had a high sequence homology to others previously identified in China, Sweden and Brazil. This is the first study conducted in the Central-West Region of Brazil to detect HBoV-1 and HBoV-3 in faecal samples from children with acute gastroenteritis. Further studies are required to define the role of HBoVs as aetiological agents of gastroenteritis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600015&lng=en&tlng=enhuman bocavirusacute gastroenteritismolecular characterisationBrazil |
| spellingShingle | Teresinha Teixeira de Sousa Menira Souza Fabíola Souza Fiaccadori Ana Maria Tavares Borges Paulo Sucasas da Costa Divina das Dôres de Paula Cardoso Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil Memorias do Instituto Oswaldo Cruz human bocavirus acute gastroenteritis molecular characterisation Brazil |
| title | Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil |
| title_full | Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil |
| title_fullStr | Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil |
| title_full_unstemmed | Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil |
| title_short | Human bocavirus 1 and 3 infection in children with acute gastroenteritis in Brazil |
| title_sort | human bocavirus 1 and 3 infection in children with acute gastroenteritis in brazil |
| topic | human bocavirus acute gastroenteritis molecular characterisation Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000600015&lng=en&tlng=en |
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