Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts
The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature,...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/21/6449 |
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| author | Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth |
| author_facet | Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth |
| author_sort | Csongor Bakó |
| collection | DOAJ |
| description | The extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The highest inhibition zone was 7.51 mm for <i>P. aeruginosa</i> and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry. |
| first_indexed | 2024-03-10T05:55:59Z |
| format | Article |
| id | doaj.art-8e67885666a243d986669df466f716ef |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:55:59Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8e67885666a243d986669df466f716ef2023-11-22T21:21:30ZengMDPI AGMolecules1420-30492021-10-012621644910.3390/molecules26216449Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid ExtractsCsongor Bakó0Viktória Lilla Balázs1Gyöngyi Takács2József Péter Pallos3Szilárd Pál4Béla Kocsis5Dóra Rippelné Pethő6Györgyi Horváth7Department of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, HungaryDepartment of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, HungaryInstitute of Chemical Engineering and Process Engineering, Faculty of Engineering, University of Pannonia, H-8200 Veszprém, HungaryPannonpharma Ltd., H-7720 Pécsvárad, HungaryDepartment of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, HungaryDepartment of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, HungaryInstitute of Chemical Engineering and Process Engineering, Faculty of Engineering, University of Pannonia, H-8200 Veszprém, HungaryDepartment of Pharmacognosy, Faculty of Pharmacy, University of Pécs, H-7624 Pécs, HungaryThe extraction of clary sage (<i>Salvia sclarea</i> L.) using supercritical carbon dioxide (SC-CO<sub>2</sub>) was systematically studied by using thin layer chromatography-direct bioautography (TLC-DB) and response surface methodology (RSM). The three parameters temperature, pressure, and cosolvent ratio were optimized for the maximum antibacterial activity of clary sage extracts against <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). The highest inhibition zone was 7.51 mm for <i>P. aeruginosa</i> and 7.57 mm for MRSA. According to RSM analysis, the predicted optimum extraction parameters are 18.6 MPa pressure, 40 °C temperature, and 2% ethanol (EtOH) ratio. The combination of this analytical and statistical method allows saving time, money, and instrument runtime in the optimization of essential oil composition, which is tailored to a specific task and could be useful on any kind of herbs in a wide range of use from perfume manufacturing to the food industry.https://www.mdpi.com/1420-3049/26/21/6449essential oilsclary sagesupercritical fluid extractionthin layer chromatography-direct bioautographyresponse surface method |
| spellingShingle | Csongor Bakó Viktória Lilla Balázs Gyöngyi Takács József Péter Pallos Szilárd Pál Béla Kocsis Dóra Rippelné Pethő Györgyi Horváth Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts Molecules essential oils clary sage supercritical fluid extraction thin layer chromatography-direct bioautography response surface method |
| title | Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts |
| title_full | Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts |
| title_fullStr | Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts |
| title_full_unstemmed | Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts |
| title_short | Combination of Analytical and Statistical Methods in Order to Optimize Antibacterial Activity of Clary Sage Supercritical Fluid Extracts |
| title_sort | combination of analytical and statistical methods in order to optimize antibacterial activity of clary sage supercritical fluid extracts |
| topic | essential oils clary sage supercritical fluid extraction thin layer chromatography-direct bioautography response surface method |
| url | https://www.mdpi.com/1420-3049/26/21/6449 |
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