A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner.
Undernutrition during pregnancy has been associated to increased vulnerability to develop metabolic and behavior alterations later in life. The endocannabinoid system might play an important role in these processes. Therefore, we investigated the effects of a moderate maternal calorie-restricted die...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5367805?pdf=render |
| _version_ | 1828918068261158912 |
|---|---|
| author | María Teresa Ramírez-López Mariam Vázquez Ermelinda Lomazzo Clementine Hofmann Rosario Noemi Blanco Francisco Alén María Antón Juan Decara Rocío Arco Laura Orio Juan Suárez Beat Lutz Raquel Gómez de Heras Laura Bindila Fernando Rodríguez de Fonseca |
| author_facet | María Teresa Ramírez-López Mariam Vázquez Ermelinda Lomazzo Clementine Hofmann Rosario Noemi Blanco Francisco Alén María Antón Juan Decara Rocío Arco Laura Orio Juan Suárez Beat Lutz Raquel Gómez de Heras Laura Bindila Fernando Rodríguez de Fonseca |
| author_sort | María Teresa Ramírez-López |
| collection | DOAJ |
| description | Undernutrition during pregnancy has been associated to increased vulnerability to develop metabolic and behavior alterations later in life. The endocannabinoid system might play an important role in these processes. Therefore, we investigated the effects of a moderate maternal calorie-restricted diet on the levels of the endocannabinoid 2-arachidonoyl glycerol (2-AG), arachidonic acid (AA) and the N-acylethanolamines (NAEs) anandamide (AEA), oleoylethanolamide (OEA) and palmitoylethanolamide (PEA) in the brain of newborn rat offspring. We focused on brain structures involved in metabolism, feeding behavior, as well as emotional and cognitive responses. Female Wistar rats were assigned during the entire pregnancy to either control diet (C) or restriction diet (R), consisting of a 20% calorie-restricted diet. Weight gain and caloric intake of rat dams were monitored and birth outcomes were assessed. 2-AG, AA and NAE levels were measured in hypothalamus, hippocampus and olfactory bulb of the offspring. R dams displayed lower gain weight from the middle pregnancy and consumed less calories during the entire pregnancy. Offspring from R dams were underweight at birth, but litter size was unaffected. In hypothalamus, R male offspring displayed decreased levels of AA and OEA, with no change in the levels of the endocannabinoids 2-AG and AEA. R female exhibited decreased 2-AG and PEA levels. The opposite was found in the hippocampus, where R male displayed increased 2-AG and AA levels, and R female exhibited elevated levels of AEA, AA and PEA. In the olfactory bulb, only R female presented decreased levels of AEA, AA and PEA. Therefore, a moderate diet restriction during the entire pregnancy alters differentially the endocannabinoids and/or endocannabinoid-related lipids in hypothalamus and hippocampus of the underweight offspring, similarly in both sexes, whereas sex-specific alterations occur in the olfactory bulb. Consequently, endocannabinoid and endocannabinoid-related lipid signaling alterations might be involved in the long-term and sexual dimorphism effects commonly observed after undernutrition and low birth weight. |
| first_indexed | 2024-12-13T21:02:56Z |
| format | Article |
| id | doaj.art-8e6e19443866443eb11592648bded30b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:02:56Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8e6e19443866443eb11592648bded30b2022-12-21T23:31:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017430710.1371/journal.pone.0174307A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner.María Teresa Ramírez-LópezMariam VázquezErmelinda LomazzoClementine HofmannRosario Noemi BlancoFrancisco AlénMaría AntónJuan DecaraRocío ArcoLaura OrioJuan SuárezBeat LutzRaquel Gómez de HerasLaura BindilaFernando Rodríguez de FonsecaUndernutrition during pregnancy has been associated to increased vulnerability to develop metabolic and behavior alterations later in life. The endocannabinoid system might play an important role in these processes. Therefore, we investigated the effects of a moderate maternal calorie-restricted diet on the levels of the endocannabinoid 2-arachidonoyl glycerol (2-AG), arachidonic acid (AA) and the N-acylethanolamines (NAEs) anandamide (AEA), oleoylethanolamide (OEA) and palmitoylethanolamide (PEA) in the brain of newborn rat offspring. We focused on brain structures involved in metabolism, feeding behavior, as well as emotional and cognitive responses. Female Wistar rats were assigned during the entire pregnancy to either control diet (C) or restriction diet (R), consisting of a 20% calorie-restricted diet. Weight gain and caloric intake of rat dams were monitored and birth outcomes were assessed. 2-AG, AA and NAE levels were measured in hypothalamus, hippocampus and olfactory bulb of the offspring. R dams displayed lower gain weight from the middle pregnancy and consumed less calories during the entire pregnancy. Offspring from R dams were underweight at birth, but litter size was unaffected. In hypothalamus, R male offspring displayed decreased levels of AA and OEA, with no change in the levels of the endocannabinoids 2-AG and AEA. R female exhibited decreased 2-AG and PEA levels. The opposite was found in the hippocampus, where R male displayed increased 2-AG and AA levels, and R female exhibited elevated levels of AEA, AA and PEA. In the olfactory bulb, only R female presented decreased levels of AEA, AA and PEA. Therefore, a moderate diet restriction during the entire pregnancy alters differentially the endocannabinoids and/or endocannabinoid-related lipids in hypothalamus and hippocampus of the underweight offspring, similarly in both sexes, whereas sex-specific alterations occur in the olfactory bulb. Consequently, endocannabinoid and endocannabinoid-related lipid signaling alterations might be involved in the long-term and sexual dimorphism effects commonly observed after undernutrition and low birth weight.http://europepmc.org/articles/PMC5367805?pdf=render |
| spellingShingle | María Teresa Ramírez-López Mariam Vázquez Ermelinda Lomazzo Clementine Hofmann Rosario Noemi Blanco Francisco Alén María Antón Juan Decara Rocío Arco Laura Orio Juan Suárez Beat Lutz Raquel Gómez de Heras Laura Bindila Fernando Rodríguez de Fonseca A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. PLoS ONE |
| title | A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. |
| title_full | A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. |
| title_fullStr | A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. |
| title_full_unstemmed | A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. |
| title_short | A moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid-related lipids in the hypothalamus, hippocampus and olfactory bulb of rat offspring in a sex-specific manner. |
| title_sort | moderate diet restriction during pregnancy alters the levels of endocannabinoids and endocannabinoid related lipids in the hypothalamus hippocampus and olfactory bulb of rat offspring in a sex specific manner |
| url | http://europepmc.org/articles/PMC5367805?pdf=render |
| work_keys_str_mv | AT mariateresaramirezlopez amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT mariamvazquez amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT ermelindalomazzo amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT clementinehofmann amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT rosarionoemiblanco amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT franciscoalen amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT mariaanton amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT juandecara amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT rocioarco amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT lauraorio amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT juansuarez amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT beatlutz amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT raquelgomezdeheras amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT laurabindila amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT fernandorodriguezdefonseca amoderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT mariateresaramirezlopez moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT mariamvazquez moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT ermelindalomazzo moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT clementinehofmann moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT rosarionoemiblanco moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT franciscoalen moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT mariaanton moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT juandecara moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT rocioarco moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT lauraorio moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT juansuarez moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT beatlutz moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT raquelgomezdeheras moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT laurabindila moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner AT fernandorodriguezdefonseca moderatedietrestrictionduringpregnancyaltersthelevelsofendocannabinoidsandendocannabinoidrelatedlipidsinthehypothalamushippocampusandolfactorybulbofratoffspringinasexspecificmanner |