Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping
Abstract Background Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sa...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-06-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12870-017-1047-x |
| _version_ | 1811263636096679936 |
|---|---|
| author | Congjun Jia Xinming Wu Min Chen Yunqi Wang Xiqiang Liu Pan Gong Qingfang Xu Xuemin Wang Hongwen Gao Zan Wang |
| author_facet | Congjun Jia Xinming Wu Min Chen Yunqi Wang Xiqiang Liu Pan Gong Qingfang Xu Xuemin Wang Hongwen Gao Zan Wang |
| author_sort | Congjun Jia |
| collection | DOAJ |
| description | Abstract Background Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sativa) association mapping study for six traits, namely crude protein (CP), rumen undegraded protein (RUP), and four mineral elements (Ca, K, Mg and P), was conducted in three consecutive years using a large collection encompassing 336 genotypes genotyped with 85 simple sequence repeat (SSR) markers. Results All the traits were significantly influenced by genotype, environment, and genotype × environment interaction. Eight-five significant associations (P < 0.005) were identified. Among these, five associations with Ca were repeatedly observed and six co-localized associations were identified. Conclusions The identified marker alleles significantly associated with the traits provided important information for understanding genetic controls of alfalfa quality. The markers could be used in assisting selection for the individual traits in breeding populations for developing new alfalfa cultivars. |
| first_indexed | 2024-04-12T19:49:00Z |
| format | Article |
| id | doaj.art-8e70ecc6a5094a488c22b81d9ce5f697 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T19:49:00Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-8e70ecc6a5094a488c22b81d9ce5f6972022-12-22T03:18:53ZengBMCBMC Plant Biology1471-22292017-06-011711710.1186/s12870-017-1047-xIdentification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mappingCongjun Jia0Xinming Wu1Min Chen2Yunqi Wang3Xiqiang Liu4Pan Gong5Qingfang Xu6Xuemin Wang7Hongwen Gao8Zan Wang9Institute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Science, Shanxi Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Husbandry and Veterinary Science, Shanxi Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesCollege of Animal Science and Veterinary Medicine, Shanxi Agricultural UniversityInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesAbstract Background Alfalfa (Medicago sativa) is one of the most important legume forage species in China and many other countries of the world. It provides a quality source of proteins and minerals to animals. Genetic underpinnings for these important traits, however, are elusive. An alfalfa (M. sativa) association mapping study for six traits, namely crude protein (CP), rumen undegraded protein (RUP), and four mineral elements (Ca, K, Mg and P), was conducted in three consecutive years using a large collection encompassing 336 genotypes genotyped with 85 simple sequence repeat (SSR) markers. Results All the traits were significantly influenced by genotype, environment, and genotype × environment interaction. Eight-five significant associations (P < 0.005) were identified. Among these, five associations with Ca were repeatedly observed and six co-localized associations were identified. Conclusions The identified marker alleles significantly associated with the traits provided important information for understanding genetic controls of alfalfa quality. The markers could be used in assisting selection for the individual traits in breeding populations for developing new alfalfa cultivars.http://link.springer.com/article/10.1186/s12870-017-1047-xAlfalfaAssociation mappingCrude proteinMineral elementsSimple sequence repeat (SSR) |
| spellingShingle | Congjun Jia Xinming Wu Min Chen Yunqi Wang Xiqiang Liu Pan Gong Qingfang Xu Xuemin Wang Hongwen Gao Zan Wang Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping BMC Plant Biology Alfalfa Association mapping Crude protein Mineral elements Simple sequence repeat (SSR) |
| title | Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping |
| title_full | Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping |
| title_fullStr | Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping |
| title_full_unstemmed | Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping |
| title_short | Identification of genetic loci associated with crude protein and mineral concentrations in alfalfa (Medicago sativa) using association mapping |
| title_sort | identification of genetic loci associated with crude protein and mineral concentrations in alfalfa medicago sativa using association mapping |
| topic | Alfalfa Association mapping Crude protein Mineral elements Simple sequence repeat (SSR) |
| url | http://link.springer.com/article/10.1186/s12870-017-1047-x |
| work_keys_str_mv | AT congjunjia identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT xinmingwu identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT minchen identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT yunqiwang identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT xiqiangliu identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT pangong identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT qingfangxu identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT xueminwang identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT hongwengao identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping AT zanwang identificationofgeneticlociassociatedwithcrudeproteinandmineralconcentrationsinalfalfamedicagosativausingassociationmapping |