Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage
Porcine circovirus type 2 (PCV2) is a swine pathogen that causes postweaning multisystemic wasting syndrome, associated with a number of other disease symptoms in pigs. It has caused high economic losses for pig industries in China and worldwide. Vimentin is a cytoskeletal protein involved in the li...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170222001708 |
| _version_ | 1797366673832935424 |
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| author | Yanni Gao Junshuo Li Zhijian Wang Ping Jiang Juan Bai Yufeng Li Xianwei Wang |
| author_facet | Yanni Gao Junshuo Li Zhijian Wang Ping Jiang Juan Bai Yufeng Li Xianwei Wang |
| author_sort | Yanni Gao |
| collection | DOAJ |
| description | Porcine circovirus type 2 (PCV2) is a swine pathogen that causes postweaning multisystemic wasting syndrome, associated with a number of other disease symptoms in pigs. It has caused high economic losses for pig industries in China and worldwide. Vimentin is a cytoskeletal protein involved in the lifecycle of numerous viruses. In this study, the role of Vimentin in PCV2 replication in pig alveolar macrophage (3D4/21) cells was studied. A 3D4/21 cell line overexpressing Vimentin was established using a lentivirus expression system, in which PCV2 replication was significantly enhanced. Meanwhile, in the Vimentin silenced 3D4/21 cells, PCV2 replication was significantly reduced. Furthermore, we found that PCV2 infection in 3D4/21 cells promoted Vimentin phosphorylation and rearranged Vimentin distribution in cells. By inhibition of CaMKII pathway, Vimentin phosphorylation was significantly suppressed and PCV2 replication was significantly inhibited. The results in this study indicated that in 3D4/21 cells, PCV2 promoted CaMKIIγ-medicated Vimentin phosphorylation and altered its cellular distributions to accomplish its own replication. |
| first_indexed | 2024-03-08T17:07:04Z |
| format | Article |
| id | doaj.art-8e739a04b9584eadafab7c34846787f7 |
| institution | Directory Open Access Journal |
| issn | 1872-7492 |
| language | English |
| last_indexed | 2024-03-08T17:07:04Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj.art-8e739a04b9584eadafab7c34846787f72024-01-04T04:36:02ZengElsevierVirus Research1872-74922022-09-01318198842Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophageYanni Gao0Junshuo Li1Zhijian Wang2Ping Jiang3Juan Bai4Yufeng Li5Xianwei Wang6Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, ChinaKey Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China; Jiangsu Coinnovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China; Corresponding author at: Xianwei Wang, Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, ChinaPorcine circovirus type 2 (PCV2) is a swine pathogen that causes postweaning multisystemic wasting syndrome, associated with a number of other disease symptoms in pigs. It has caused high economic losses for pig industries in China and worldwide. Vimentin is a cytoskeletal protein involved in the lifecycle of numerous viruses. In this study, the role of Vimentin in PCV2 replication in pig alveolar macrophage (3D4/21) cells was studied. A 3D4/21 cell line overexpressing Vimentin was established using a lentivirus expression system, in which PCV2 replication was significantly enhanced. Meanwhile, in the Vimentin silenced 3D4/21 cells, PCV2 replication was significantly reduced. Furthermore, we found that PCV2 infection in 3D4/21 cells promoted Vimentin phosphorylation and rearranged Vimentin distribution in cells. By inhibition of CaMKII pathway, Vimentin phosphorylation was significantly suppressed and PCV2 replication was significantly inhibited. The results in this study indicated that in 3D4/21 cells, PCV2 promoted CaMKIIγ-medicated Vimentin phosphorylation and altered its cellular distributions to accomplish its own replication.http://www.sciencedirect.com/science/article/pii/S0168170222001708Porcine circovirus type 23D4/21 cellsVimentinPhosphorylationRearrangement |
| spellingShingle | Yanni Gao Junshuo Li Zhijian Wang Ping Jiang Juan Bai Yufeng Li Xianwei Wang Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage Virus Research Porcine circovirus type 2 3D4/21 cells Vimentin Phosphorylation Rearrangement |
| title | Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage |
| title_full | Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage |
| title_fullStr | Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage |
| title_full_unstemmed | Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage |
| title_short | Vimentin promotes porcine circovirus type 2 (PCV2) replication in pig alveolar macrophage |
| title_sort | vimentin promotes porcine circovirus type 2 pcv2 replication in pig alveolar macrophage |
| topic | Porcine circovirus type 2 3D4/21 cells Vimentin Phosphorylation Rearrangement |
| url | http://www.sciencedirect.com/science/article/pii/S0168170222001708 |
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