<i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients
Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of <i>Streptococcus mitis</i>, <i>Prevotella melaninogenica&...
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MDPI AG
2023-09-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/10/1194 |
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| author | Uros Tomic Nadja Nikolic Jelena Carkic Djordje Mihailovic Drago Jelovac Jelena Milasin Ana Pucar |
| author_facet | Uros Tomic Nadja Nikolic Jelena Carkic Djordje Mihailovic Drago Jelovac Jelena Milasin Ana Pucar |
| author_sort | Uros Tomic |
| collection | DOAJ |
| description | Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of <i>Streptococcus mitis</i>, <i>Prevotella melaninogenica</i>, and <i>Prevotella intermedia</i> in oral benign lesions, oral potentially malignant disorders (OPMDs), and oral squamous cell carcinomas (OSCCs) and investigate the impact of these bacteria on the expression patterns of the selected (potential) target genes (<i>PI3CA</i>/<i>AKT2</i>/<i>mTOR</i>, <i>DUSP16</i>/<i>MAPK14</i>, and <i>COX2</i>). After sample collection (25 benign lesions, 30 OPMDs, and 35 OSCCs) and DNA/RNA extraction, quantitative real-time polymerase chain reaction (qPCR) was performed to detect bacterial presence and assess relative gene expression levels in different lesion groups. <i>Prevotella melaninogenica</i> was the most prevalent of the three analyzed bacteria, with the frequency being 60% in benign lesions, 87% in OPMDs (<i>p</i> = 0.024), and 77% in OSCC. The OPMD tissues in which <i>Prevotella melaninogenica</i> was present exhibited a higher expression level of <i>AKT2</i> (<i>p</i> = 0.042). Significantly lower expression of <i>DUSP16</i> was observed in OSCC tissues containing <i>Streptococcus mitis</i> (<i>p</i> = 0.011). The obtained results indicate a substantial contribution of <i>P. melaninogenica</i> and <i>Str. mitis</i> in the pathogenesis of oral mucosal lesions, possibly via <i>AKT2</i> upregulation and <i>DUSP16</i> downregulation. |
| first_indexed | 2024-03-10T20:59:45Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:59:45Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-8e74b8d745cf418fa3dc7879b8952c5a2023-11-19T17:39:49ZengMDPI AGPathogens2076-08172023-09-011210119410.3390/pathogens12101194<i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis PatientsUros Tomic0Nadja Nikolic1Jelena Carkic2Djordje Mihailovic3Drago Jelovac4Jelena Milasin5Ana Pucar6Clinic for Periodontology and Oral Medicine, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Dentistry, Faculty of Medical Sciences Pristina, University of Pristina, 38220 Kosovska Mitrovica, SerbiaClinic for Maxillofacial Surgery, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Human Genetics, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaClinic for Periodontology and Oral Medicine, School of Dental Medicine, University of Belgrade, 11000 Belgrade, SerbiaOral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of <i>Streptococcus mitis</i>, <i>Prevotella melaninogenica</i>, and <i>Prevotella intermedia</i> in oral benign lesions, oral potentially malignant disorders (OPMDs), and oral squamous cell carcinomas (OSCCs) and investigate the impact of these bacteria on the expression patterns of the selected (potential) target genes (<i>PI3CA</i>/<i>AKT2</i>/<i>mTOR</i>, <i>DUSP16</i>/<i>MAPK14</i>, and <i>COX2</i>). After sample collection (25 benign lesions, 30 OPMDs, and 35 OSCCs) and DNA/RNA extraction, quantitative real-time polymerase chain reaction (qPCR) was performed to detect bacterial presence and assess relative gene expression levels in different lesion groups. <i>Prevotella melaninogenica</i> was the most prevalent of the three analyzed bacteria, with the frequency being 60% in benign lesions, 87% in OPMDs (<i>p</i> = 0.024), and 77% in OSCC. The OPMD tissues in which <i>Prevotella melaninogenica</i> was present exhibited a higher expression level of <i>AKT2</i> (<i>p</i> = 0.042). Significantly lower expression of <i>DUSP16</i> was observed in OSCC tissues containing <i>Streptococcus mitis</i> (<i>p</i> = 0.011). The obtained results indicate a substantial contribution of <i>P. melaninogenica</i> and <i>Str. mitis</i> in the pathogenesis of oral mucosal lesions, possibly via <i>AKT2</i> upregulation and <i>DUSP16</i> downregulation.https://www.mdpi.com/2076-0817/12/10/1194oral pathogenic bacteriachronic inflammationoral mucosal lesionsoral potentially malignant disorders<i>Prevotella melaninogenica</i><i>Streptococcus mitis</i> |
| spellingShingle | Uros Tomic Nadja Nikolic Jelena Carkic Djordje Mihailovic Drago Jelovac Jelena Milasin Ana Pucar <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients Pathogens oral pathogenic bacteria chronic inflammation oral mucosal lesions oral potentially malignant disorders <i>Prevotella melaninogenica</i> <i>Streptococcus mitis</i> |
| title | <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients |
| title_full | <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients |
| title_fullStr | <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients |
| title_full_unstemmed | <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients |
| title_short | <i>Streptococcus mitis</i> and <i>Prevotella melaninogenica</i> Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients |
| title_sort | i streptococcus mitis i and i prevotella melaninogenica i influence gene expression changes in oral mucosal lesions in periodontitis patients |
| topic | oral pathogenic bacteria chronic inflammation oral mucosal lesions oral potentially malignant disorders <i>Prevotella melaninogenica</i> <i>Streptococcus mitis</i> |
| url | https://www.mdpi.com/2076-0817/12/10/1194 |
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