Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs
During the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1266018/full |
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| author | Pengxiu Dai Tong Zou Wen Zhao Yangou Lv Dengke Gao Chenmei Ruan Xia Zhang Xinke Zhang Yihua Zhang Yihua Zhang |
| author_facet | Pengxiu Dai Tong Zou Wen Zhao Yangou Lv Dengke Gao Chenmei Ruan Xia Zhang Xinke Zhang Yihua Zhang Yihua Zhang |
| author_sort | Pengxiu Dai |
| collection | DOAJ |
| description | During the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled first and then decellularized. The drilled tissue-engineered menisci (Drilled Allogeneic Acellular Meniscus + Bone Marrow Mesenchymal Stem Cells, BMSCs) were transplanted into the knee joints of model dogs. On the basis of ensuring the mechanical properties, the number of the porosity and the cells implanted in allogeneic acellular meniscus was significantly increased. The expression levels of glycosaminoglycans and type II collagen in the drilled tissue-engineered meniscus were also improved. It was determined that the animals in the experimental group recovered well-compared with those in the control group. The graft surface was covered with new cartilage, the retraction degree was small, and the tissue remodeling was good. The surface wear of the femoral condyle and tibial plateau cartilage was light. The results of this study showed that increasing the porosity of allogeneic meniscus by drilling could not only maintain the mechanical properties of the meniscus and increase the number of implanted cells but also promote cell proliferation and differentiation. After transplantation, the drilled tissue-engineered meniscus provided a good remodeling effect in vivo and played a positive role in repairing meniscal injury, protecting articular cartilage and restoring knee joint function. |
| first_indexed | 2024-03-11T06:20:15Z |
| format | Article |
| id | doaj.art-8e7687d2ed7943f9890c76e2a3f58f86 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-11T06:20:15Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-8e7687d2ed7943f9890c76e2a3f58f862023-11-17T11:58:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-11-011010.3389/fvets.2023.12660181266018Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCsPengxiu Dai0Tong Zou1Wen Zhao2Yangou Lv3Dengke Gao4Chenmei Ruan5Xia Zhang6Xinke Zhang7Yihua Zhang8Yihua Zhang9Shaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaShaanxi Branch of National Stem Cell Engineering and Technology Centre, College of Veterinary Medicine, Northwest A&F University, Xianyang, Shaanxi, ChinaBaiopai (Tianjin) Biotechnology Co., LTD, Tianjin, ChinaDuring the construction of tissue-engineered meniscus, the low porosity of extracellular matrix restricts the flow of nutrient solution and the migration and proliferation of cells, thus affecting the tissue remodeling after transplantation. In this study, the canine allogeneic meniscus was drilled first and then decellularized. The drilled tissue-engineered menisci (Drilled Allogeneic Acellular Meniscus + Bone Marrow Mesenchymal Stem Cells, BMSCs) were transplanted into the knee joints of model dogs. On the basis of ensuring the mechanical properties, the number of the porosity and the cells implanted in allogeneic acellular meniscus was significantly increased. The expression levels of glycosaminoglycans and type II collagen in the drilled tissue-engineered meniscus were also improved. It was determined that the animals in the experimental group recovered well-compared with those in the control group. The graft surface was covered with new cartilage, the retraction degree was small, and the tissue remodeling was good. The surface wear of the femoral condyle and tibial plateau cartilage was light. The results of this study showed that increasing the porosity of allogeneic meniscus by drilling could not only maintain the mechanical properties of the meniscus and increase the number of implanted cells but also promote cell proliferation and differentiation. After transplantation, the drilled tissue-engineered meniscus provided a good remodeling effect in vivo and played a positive role in repairing meniscal injury, protecting articular cartilage and restoring knee joint function.https://www.frontiersin.org/articles/10.3389/fvets.2023.1266018/fulldrilled allogeneic acellular meniscusporosityBMSCsdrilled tissue-engineered meniscustransplantation therapy |
| spellingShingle | Pengxiu Dai Tong Zou Wen Zhao Yangou Lv Dengke Gao Chenmei Ruan Xia Zhang Xinke Zhang Yihua Zhang Yihua Zhang Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs Frontiers in Veterinary Science drilled allogeneic acellular meniscus porosity BMSCs drilled tissue-engineered meniscus transplantation therapy |
| title | Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs |
| title_full | Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs |
| title_fullStr | Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs |
| title_full_unstemmed | Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs |
| title_short | Short-term transplantation effect of a tissue-engineered meniscus constructed using drilled allogeneic acellular meniscus and BMSCs |
| title_sort | short term transplantation effect of a tissue engineered meniscus constructed using drilled allogeneic acellular meniscus and bmscs |
| topic | drilled allogeneic acellular meniscus porosity BMSCs drilled tissue-engineered meniscus transplantation therapy |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1266018/full |
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