Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay
Laccase is a copper-containing enzyme that does not require hydrogen peroxide as a co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction of enzyme-based biosensors. Metals, metal oxides, semiconductors, an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/741 |
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| author | Olha Demkiv Galina Gayda Nataliya Stasyuk Olena Brahinetz Mykhailo Gonchar Marina Nisnevitch |
| author_facet | Olha Demkiv Galina Gayda Nataliya Stasyuk Olena Brahinetz Mykhailo Gonchar Marina Nisnevitch |
| author_sort | Olha Demkiv |
| collection | DOAJ |
| description | Laccase is a copper-containing enzyme that does not require hydrogen peroxide as a co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction of enzyme-based biosensors. Metals, metal oxides, semiconductors, and composite NPs perform various functions in electrochemical transformation schemes as a platform for the enzyme immobilization, a mediator of an electron transfer, and a signal amplifier. We describe here the development of amperometric biosensors (ABSs) based on laccase and redox-active micro/nanoparticles (hereafter—NPs), which were immobilized on a graphite electrode (GE). For this purpose, we isolated a highly purified enzyme from the fungus <i>Trametes zonatus</i>, and then synthesized bi- and trimetallic NPs of noble and transition metals, as well as hexacyanoferrates (HCF) of noble metals; these were layered onto the surfaces of GEs. The electroactivity of many of the NPs immobilized on the GEs was characterized by cyclic voltammetry (CV) experiments. The most effective mediators of electron transfer were selected as the platform for the development of laccase-based ABSs. As a result, a number of catechol-sensitive ABSs were constructed and characterized. The laccase/CuCo/GE was demonstrated to possess the highest sensitivity to catechol (4523 A·M<sup>−1</sup>·m<sup>−2</sup>) among the tested ABSs. The proposed ABSs may be promising for the analysis of phenolic derivatives in real samples of drinking water, wastewater, and food products. |
| first_indexed | 2024-03-10T00:35:30Z |
| format | Article |
| id | doaj.art-8e7d1d1ce9b44c649d4e8ab0f9ed681a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:35:30Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-8e7d1d1ce9b44c649d4e8ab0f9ed681a2023-11-23T15:18:23ZengMDPI AGBiosensors2079-63742022-09-0112974110.3390/bios12090741Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol AssayOlha Demkiv0Galina Gayda1Nataliya Stasyuk2Olena Brahinetz3Mykhailo Gonchar4Marina Nisnevitch5Institute of Cell Biology National Academy of Sciences of Ukraine, 14/16, Dragomanova Str., 79005 Lviv, UkraineInstitute of Cell Biology National Academy of Sciences of Ukraine, 14/16, Dragomanova Str., 79005 Lviv, UkraineInstitute of Cell Biology National Academy of Sciences of Ukraine, 14/16, Dragomanova Str., 79005 Lviv, UkraineState Institution Institute of Blood Pathology and Transfusion Medicine National Academy of Medical Sciences of Ukraine, 45, General Chuprinka Str., 79044 Lviv, UkraineInstitute of Cell Biology National Academy of Sciences of Ukraine, 14/16, Dragomanova Str., 79005 Lviv, UkraineDepartment of Chemical Engineering, Ariel University, Kyriat-ha-Mada, Ariel 4070000, IsraelLaccase is a copper-containing enzyme that does not require hydrogen peroxide as a co-substrate or additional cofactors for an enzymatic reaction. Nanomaterials of various chemical structures are usually applied to the construction of enzyme-based biosensors. Metals, metal oxides, semiconductors, and composite NPs perform various functions in electrochemical transformation schemes as a platform for the enzyme immobilization, a mediator of an electron transfer, and a signal amplifier. We describe here the development of amperometric biosensors (ABSs) based on laccase and redox-active micro/nanoparticles (hereafter—NPs), which were immobilized on a graphite electrode (GE). For this purpose, we isolated a highly purified enzyme from the fungus <i>Trametes zonatus</i>, and then synthesized bi- and trimetallic NPs of noble and transition metals, as well as hexacyanoferrates (HCF) of noble metals; these were layered onto the surfaces of GEs. The electroactivity of many of the NPs immobilized on the GEs was characterized by cyclic voltammetry (CV) experiments. The most effective mediators of electron transfer were selected as the platform for the development of laccase-based ABSs. As a result, a number of catechol-sensitive ABSs were constructed and characterized. The laccase/CuCo/GE was demonstrated to possess the highest sensitivity to catechol (4523 A·M<sup>−1</sup>·m<sup>−2</sup>) among the tested ABSs. The proposed ABSs may be promising for the analysis of phenolic derivatives in real samples of drinking water, wastewater, and food products.https://www.mdpi.com/2079-6374/12/9/741laccaseelectroactive nanoparticlesamperometric biosensorcatechol analysis |
| spellingShingle | Olha Demkiv Galina Gayda Nataliya Stasyuk Olena Brahinetz Mykhailo Gonchar Marina Nisnevitch Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay Biosensors laccase electroactive nanoparticles amperometric biosensor catechol analysis |
| title | Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay |
| title_full | Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay |
| title_fullStr | Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay |
| title_full_unstemmed | Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay |
| title_short | Nanomaterials as Redox Mediators in Laccase-Based Amperometric Biosensors for Catechol Assay |
| title_sort | nanomaterials as redox mediators in laccase based amperometric biosensors for catechol assay |
| topic | laccase electroactive nanoparticles amperometric biosensor catechol analysis |
| url | https://www.mdpi.com/2079-6374/12/9/741 |
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