DMSO Improves the Ski-Slope Effect in Direct PCR
Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA profiles efficiently. However, non-specific amplific...
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MDPI AG
2021-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/4/1943 |
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| author | Joo-Young Kim Ju Yeon Jung Da-Hye Kim Seohyun Moon Won-Hae Lee Byung-Won Chun Dong-Ho Choi |
| author_facet | Joo-Young Kim Ju Yeon Jung Da-Hye Kim Seohyun Moon Won-Hae Lee Byung-Won Chun Dong-Ho Choi |
| author_sort | Joo-Young Kim |
| collection | DOAJ |
| description | Analytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA profiles efficiently. However, non-specific amplification may occur depending on the quality of the crime scene evidence and amplification methods employed. In particular, the ski-slope effect observed in direct PCR amplification has led to inaccurate interpretations of the DNA profile results. In this study, we aimed to reduce the ski-slope effect by using dimethyl sulfoxide (DMSO) in direct PCR. We confirmed that DMSO (3.75%, <i>v</i>/<i>v</i>) increased the amplification yield of large-sized DNA sequences more than that of small-sized ones. Using 50 Korean buccal samples, we further demonstrated that DMSO reduced the ski-slope effect in direct PCR. These results suggest that the experimental method developed in this study is suitable for direct PCR and may help to successfully obtain DNA profiles from various types of evidence at crime scenes. |
| first_indexed | 2024-03-09T00:37:37Z |
| format | Article |
| id | doaj.art-8e7e5ff2a7714c1da54fc59b13d5bace |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T00:37:37Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-8e7e5ff2a7714c1da54fc59b13d5bace2023-12-11T18:03:12ZengMDPI AGApplied Sciences2076-34172021-02-01114194310.3390/app11041943DMSO Improves the Ski-Slope Effect in Direct PCRJoo-Young Kim0Ju Yeon Jung1Da-Hye Kim2Seohyun Moon3Won-Hae Lee4Byung-Won Chun5Dong-Ho Choi6Forensic DNA Division, National Forensic Service, Wonju 26460, KoreaForensic DNA Division, National Forensic Service, Wonju 26460, KoreaForensic DNA Division, National Forensic Service, Wonju 26460, KoreaForensic DNA Division, National Forensic Service, Wonju 26460, KoreaForensic DNA Division, National Forensic Service, Wonju 26460, KoreaDNA Analysis Division, National Forensic Service, Busan Institute, Busan 50612, KoreaDNA Analysis Division, National Forensic Service, Busan Institute, Busan 50612, KoreaAnalytical techniques such as DNA profiling are widely used in various fields, including forensic science, and novel technologies such as direct polymerase chain reaction (PCR) amplification are continuously being developed in order to acquire DNA profiles efficiently. However, non-specific amplification may occur depending on the quality of the crime scene evidence and amplification methods employed. In particular, the ski-slope effect observed in direct PCR amplification has led to inaccurate interpretations of the DNA profile results. In this study, we aimed to reduce the ski-slope effect by using dimethyl sulfoxide (DMSO) in direct PCR. We confirmed that DMSO (3.75%, <i>v</i>/<i>v</i>) increased the amplification yield of large-sized DNA sequences more than that of small-sized ones. Using 50 Korean buccal samples, we further demonstrated that DMSO reduced the ski-slope effect in direct PCR. These results suggest that the experimental method developed in this study is suitable for direct PCR and may help to successfully obtain DNA profiles from various types of evidence at crime scenes.https://www.mdpi.com/2076-3417/11/4/1943direct PCRshort tandem repeatski-slope effectDMSOforensic DNA |
| spellingShingle | Joo-Young Kim Ju Yeon Jung Da-Hye Kim Seohyun Moon Won-Hae Lee Byung-Won Chun Dong-Ho Choi DMSO Improves the Ski-Slope Effect in Direct PCR Applied Sciences direct PCR short tandem repeat ski-slope effect DMSO forensic DNA |
| title | DMSO Improves the Ski-Slope Effect in Direct PCR |
| title_full | DMSO Improves the Ski-Slope Effect in Direct PCR |
| title_fullStr | DMSO Improves the Ski-Slope Effect in Direct PCR |
| title_full_unstemmed | DMSO Improves the Ski-Slope Effect in Direct PCR |
| title_short | DMSO Improves the Ski-Slope Effect in Direct PCR |
| title_sort | dmso improves the ski slope effect in direct pcr |
| topic | direct PCR short tandem repeat ski-slope effect DMSO forensic DNA |
| url | https://www.mdpi.com/2076-3417/11/4/1943 |
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