Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals
Background Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the...
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PeerJ Inc.
2016-09-01
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| author | Christiana Jesumirhewe Peter Oladejo Ogunlowo Mitsan Olley Burkhard Springer Franz Allerberger Werner Ruppitsch |
| author_facet | Christiana Jesumirhewe Peter Oladejo Ogunlowo Mitsan Olley Burkhard Springer Franz Allerberger Werner Ruppitsch |
| author_sort | Christiana Jesumirhewe |
| collection | DOAJ |
| description | Background Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria. In this study, matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technique was compared to conventional identification techniques. Materials and Methods In total, 147 Enterobacteriaceae isolates were collected from March to May 2015 from three medical microbiology laboratories of hospitals in Edo state, Nigeria, after being tested according to the individual laboratories standard operating procedures. All isolates were stored at −20°C until tested centrally by MALDI-TOF MS. Results One hundred and forty five (98.6%) isolates had a MALDI Biotyper best score > or =2.0, indicating a secure genus and probable species identification; and 2(1.36%) isolates had a best score <2.0 indicating probable genus identification. Isolates with best scores of > or =2.0 comprised nine genera and 10 species, respectively. A total of 57.2% and 33.1% of isolates identified had agreement between MALDI-TOF MS and conventional techniques for identification at genus and species level, respectively, when analyzing bacteria with MALDI Biotyper best scores > or =2.0. Conclusion The results of our study show that the applied conventional identification techniques for Enterobacteriaceae in the investigated Nigerian hospitals are not very accurate. Use of state-of-the-art identification technologies for microorganisms is necessary to guarantee comparability of bacteriological results. |
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| spelling | doaj.art-8e80a249f2244c3c8ac7870c6d48692e2023-12-03T10:52:45ZengPeerJ Inc.PeerJ2167-83592016-09-014e251110.7717/peerj.2511Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitalsChristiana Jesumirhewe0Peter Oladejo Ogunlowo1Mitsan Olley2Burkhard Springer3Franz Allerberger4Werner Ruppitsch5Department of Pharmaceutical Microbiology, Igbinedion University Okada, Okada, Edo state, NigeriaDepartment of Pharmaceutical Microbiology, Igbinedion University Okada, Okada, Edo state, NigeriaDepartment of Medical Microbiology, Igbinedion Univesity Teaching Hospital, Okada, Edo state, NigeriaAustrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene, Vienna, AustriaAustrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene, Vienna, AustriaAustrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene, Vienna, AustriaBackground Enterobacteriaceae are ubiquitously present in nature and can be found in the intestinal tract of humans and animals as commensal flora. Multidrug-resistant Enterobacteriaceae are increasingly reported and are a threat to public health implicating a need for accurate identification of the isolates to species level. In developing countries, identification of bacteria basically depends on conventional methods: culture and phenotypic methods that hamper the accurate identification of bacteria. In this study, matrix-assisted desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) technique was compared to conventional identification techniques. Materials and Methods In total, 147 Enterobacteriaceae isolates were collected from March to May 2015 from three medical microbiology laboratories of hospitals in Edo state, Nigeria, after being tested according to the individual laboratories standard operating procedures. All isolates were stored at −20°C until tested centrally by MALDI-TOF MS. Results One hundred and forty five (98.6%) isolates had a MALDI Biotyper best score > or =2.0, indicating a secure genus and probable species identification; and 2(1.36%) isolates had a best score <2.0 indicating probable genus identification. Isolates with best scores of > or =2.0 comprised nine genera and 10 species, respectively. A total of 57.2% and 33.1% of isolates identified had agreement between MALDI-TOF MS and conventional techniques for identification at genus and species level, respectively, when analyzing bacteria with MALDI Biotyper best scores > or =2.0. Conclusion The results of our study show that the applied conventional identification techniques for Enterobacteriaceae in the investigated Nigerian hospitals are not very accurate. Use of state-of-the-art identification technologies for microorganisms is necessary to guarantee comparability of bacteriological results.https://peerj.com/articles/2511.pdfEnterobacteriaceaeBacterial identificationMALDI-TOF MS |
| spellingShingle | Christiana Jesumirhewe Peter Oladejo Ogunlowo Mitsan Olley Burkhard Springer Franz Allerberger Werner Ruppitsch Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals PeerJ Enterobacteriaceae Bacterial identification MALDI-TOF MS |
| title | Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals |
| title_full | Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals |
| title_fullStr | Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals |
| title_full_unstemmed | Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals |
| title_short | Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals |
| title_sort | accuracy of conventional identification methods used for enterobacteriaceae isolates in three nigerian hospitals |
| topic | Enterobacteriaceae Bacterial identification MALDI-TOF MS |
| url | https://peerj.com/articles/2511.pdf |
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