Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize
Proteins post-translational modification (PTMs) is necessary in the whole life process of organisms. Among them, lysine 2-hydroxyisobutyrylation (Khib) plays an important role in protein synthesis, transcriptional regulation, and cell metabolism. Khib is a newly identified PTM in several plant speci...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1000039/full |
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| author | Kang Zhang Kang Zhang Hongzhe Cao Hongzhe Cao Yuxin Ma Helong Si Jinping Zang Hua Bai Lu Yu Xi Pang Fan Zhou Jihong Xing Jihong Xing Jingao Dong Jingao Dong |
| author_facet | Kang Zhang Kang Zhang Hongzhe Cao Hongzhe Cao Yuxin Ma Helong Si Jinping Zang Hua Bai Lu Yu Xi Pang Fan Zhou Jihong Xing Jihong Xing Jingao Dong Jingao Dong |
| author_sort | Kang Zhang |
| collection | DOAJ |
| description | Proteins post-translational modification (PTMs) is necessary in the whole life process of organisms. Among them, lysine 2-hydroxyisobutyrylation (Khib) plays an important role in protein synthesis, transcriptional regulation, and cell metabolism. Khib is a newly identified PTM in several plant species. However, the function of Khib in maize was unclear. In this study, western blotting results showed that Khib modification level increased significantly after Fusarium graminearum infection, and 2,066 Khib modified sites on 728 proteins were identified in maize, among which 24 Khib sites occurred on core histones. Subcellular localization results showed that these Khib modified proteins were localized in cytoplasm, chloroplast, and nucleus. Then, comparative proteomic analysis of the defense response to F. graminearum infection showed that Khib modification participated in plant resistance to pathogen infection by regulating glycolysis, TCA cycle, protein synthesis, peroxisome, and secondary metabolic processes, such as benzoxazinoid biosynthesis, phenylpropanoid biosynthesis, jasmonic acid synthesis, and tyrosine and tryptophan biosynthesis. In addition, we also demonstrated that lysine 2-hydroxyisobutyrylation sites on histones were involved in the gene expression of pathogenesis-related proteins. Our results provide a new perspective for the study of plant disease resistance, and had directive significance of maize disease resistance for molecular breeding. |
| first_indexed | 2024-04-11T21:08:55Z |
| format | Article |
| id | doaj.art-8e87287582a94eb1991fddb831209f6d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T21:08:55Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-8e87287582a94eb1991fddb831209f6d2022-12-22T04:03:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.10000391000039Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maizeKang Zhang0Kang Zhang1Hongzhe Cao2Hongzhe Cao3Yuxin Ma4Helong Si5Jinping Zang6Hua Bai7Lu Yu8Xi Pang9Fan Zhou10Jihong Xing11Jihong Xing12Jingao Dong13Jingao Dong14State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaState Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding, Hebei, ChinaHebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding, Hebei, ChinaProteins post-translational modification (PTMs) is necessary in the whole life process of organisms. Among them, lysine 2-hydroxyisobutyrylation (Khib) plays an important role in protein synthesis, transcriptional regulation, and cell metabolism. Khib is a newly identified PTM in several plant species. However, the function of Khib in maize was unclear. In this study, western blotting results showed that Khib modification level increased significantly after Fusarium graminearum infection, and 2,066 Khib modified sites on 728 proteins were identified in maize, among which 24 Khib sites occurred on core histones. Subcellular localization results showed that these Khib modified proteins were localized in cytoplasm, chloroplast, and nucleus. Then, comparative proteomic analysis of the defense response to F. graminearum infection showed that Khib modification participated in plant resistance to pathogen infection by regulating glycolysis, TCA cycle, protein synthesis, peroxisome, and secondary metabolic processes, such as benzoxazinoid biosynthesis, phenylpropanoid biosynthesis, jasmonic acid synthesis, and tyrosine and tryptophan biosynthesis. In addition, we also demonstrated that lysine 2-hydroxyisobutyrylation sites on histones were involved in the gene expression of pathogenesis-related proteins. Our results provide a new perspective for the study of plant disease resistance, and had directive significance of maize disease resistance for molecular breeding.https://www.frontiersin.org/articles/10.3389/fpls.2022.1000039/fullmaizeproteomiclysine 2-hydroxyisobutyrylationhistone modificationdisease resistance |
| spellingShingle | Kang Zhang Kang Zhang Hongzhe Cao Hongzhe Cao Yuxin Ma Helong Si Jinping Zang Hua Bai Lu Yu Xi Pang Fan Zhou Jihong Xing Jihong Xing Jingao Dong Jingao Dong Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize Frontiers in Plant Science maize proteomic lysine 2-hydroxyisobutyrylation histone modification disease resistance |
| title | Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize |
| title_full | Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize |
| title_fullStr | Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize |
| title_full_unstemmed | Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize |
| title_short | Global analysis of lysine 2-hydroxyisobutyrylation during Fusarium graminearum infection in maize |
| title_sort | global analysis of lysine 2 hydroxyisobutyrylation during fusarium graminearum infection in maize |
| topic | maize proteomic lysine 2-hydroxyisobutyrylation histone modification disease resistance |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1000039/full |
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