Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis
Objective: Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammationof the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in thepathogenesis of RA. In addition, the anti-inflammatory and modulatory effec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-07-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_705038_2764dddc6a89a05fafa0de13c8924b8f.pdf |
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| author | Hanieh Mahi Zahra Yousefi Fatemeh Toufan Maryam Yarmohammadi Moslem Jafarisani Nahid Eskandari Mohammad- Hossein Shams Reza Jafari |
| author_facet | Hanieh Mahi Zahra Yousefi Fatemeh Toufan Maryam Yarmohammadi Moslem Jafarisani Nahid Eskandari Mohammad- Hossein Shams Reza Jafari |
| author_sort | Hanieh Mahi |
| collection | DOAJ |
| description | Objective: Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammationof the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in thepathogenesis of RA. In addition, the anti-inflammatory and modulatory effects of oleuropein (OLEU) on the expressionpattern of microRNAs (miRNAs) have been shown in different diseases. Therefore, this study aimed to evaluate boththe sensitivity and specificity of miRNA-146a and determine the potential effects of OLEU on the expression levels ofmiRNA-146a and tumour necrosis factor-alpha (TNF-α) in RA patients.Materials and Methods: The participants in this experimental study were divided into 2 groups: RA (n=45) and healthycontrols (n=30). The isolated peripheral blood mononuclear cells (PBMCs) were treated with different concentrationsof OLEU; and the level of TNF-α expression, anti-citrullinated protein, and miRNA-146a were determined usingenzyme-linked immunoassay and real-time polymerase chain reaction, respectively. In addition, the receiver operatingcharacteristic (ROC) curve analysis evaluated the sensitivity and specificity of miRNA-146a in RA patients.Results: Results revealed a positive correlation between the levels of miRNA-146a expression with the serum levels ofC-reactive protein (CRP) and rheumatoid factor (RF) in RA patients. In addition, OLEU treatment decreased the levelsof TNF-α and miRNA-146a expression in treated PBMCs samples compared with untreated cells. The ROC curveanalysis showed an 85% sensitivity and 100% specificity of miRNA-146a in RA patients.Conclusion: Therefore, miRNA-146a can be used as a useful biomarker for RA diagnosis, particularly for earlydetection. In addition, OLEU could suppress inflammation in RA patients through the regulation of miRNA-146a. |
| first_indexed | 2024-03-12T17:10:25Z |
| format | Article |
| id | doaj.art-8e876c42787043368b92c0ec48c3505d |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-03-12T17:10:25Z |
| publishDate | 2023-07-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-8e876c42787043368b92c0ec48c3505d2023-08-06T10:58:44ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-07-0125750551210.22074/cellj.2023.1989941.1237705038Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid ArthritisHanieh Mahi0Zahra Yousefi1Fatemeh Toufan2Maryam Yarmohammadi3Moslem Jafarisani4Nahid Eskandari5Mohammad- Hossein Shams6Reza Jafari7Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranSchools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, IranStudent Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranStudent Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, IranSchools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, IranDepartment of Immunology, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Immunology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, IranSchools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, IranObjective: Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammationof the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in thepathogenesis of RA. In addition, the anti-inflammatory and modulatory effects of oleuropein (OLEU) on the expressionpattern of microRNAs (miRNAs) have been shown in different diseases. Therefore, this study aimed to evaluate boththe sensitivity and specificity of miRNA-146a and determine the potential effects of OLEU on the expression levels ofmiRNA-146a and tumour necrosis factor-alpha (TNF-α) in RA patients.Materials and Methods: The participants in this experimental study were divided into 2 groups: RA (n=45) and healthycontrols (n=30). The isolated peripheral blood mononuclear cells (PBMCs) were treated with different concentrationsof OLEU; and the level of TNF-α expression, anti-citrullinated protein, and miRNA-146a were determined usingenzyme-linked immunoassay and real-time polymerase chain reaction, respectively. In addition, the receiver operatingcharacteristic (ROC) curve analysis evaluated the sensitivity and specificity of miRNA-146a in RA patients.Results: Results revealed a positive correlation between the levels of miRNA-146a expression with the serum levels ofC-reactive protein (CRP) and rheumatoid factor (RF) in RA patients. In addition, OLEU treatment decreased the levelsof TNF-α and miRNA-146a expression in treated PBMCs samples compared with untreated cells. The ROC curveanalysis showed an 85% sensitivity and 100% specificity of miRNA-146a in RA patients.Conclusion: Therefore, miRNA-146a can be used as a useful biomarker for RA diagnosis, particularly for earlydetection. In addition, OLEU could suppress inflammation in RA patients through the regulation of miRNA-146a.https://www.celljournal.org/article_705038_2764dddc6a89a05fafa0de13c8924b8f.pdfanti-citrullinated proteinmirna-146aoleuropeinrheumatoid arthritistumour necrosis factor-alpha |
| spellingShingle | Hanieh Mahi Zahra Yousefi Fatemeh Toufan Maryam Yarmohammadi Moslem Jafarisani Nahid Eskandari Mohammad- Hossein Shams Reza Jafari Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis Cell Journal anti-citrullinated protein mirna-146a oleuropein rheumatoid arthritis tumour necrosis factor-alpha |
| title | Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis |
| title_full | Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis |
| title_fullStr | Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis |
| title_full_unstemmed | Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis |
| title_short | Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis |
| title_sort | oleuropein as an effective suppressor of inflammation and microrna 146a expression in patients with rheumatoid arthritis |
| topic | anti-citrullinated protein mirna-146a oleuropein rheumatoid arthritis tumour necrosis factor-alpha |
| url | https://www.celljournal.org/article_705038_2764dddc6a89a05fafa0de13c8924b8f.pdf |
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